1. Micro/Nano Technology Center Orientation and Safety Training University of Louisville

2. This Orientation Is meant to be an introduction to the manual Is going to teach you basic rules and regulations, preventative safety measures, and how to respond to safety incidences. Will show you where to get more information about topics covered in this presentation

3. The Manual Has information about general procedures, rules for working in the cleanroom and safety procedures in the cleanroom. Should answer most of your questions. If you can’t find something or have a question let us know!

4. Responsibilities As a cleanroom user, you must follow all of the rules listed in the manual. If you fail to follow these rules, consequences will happen – including the potential loss of cleanroom access.

5. GENERAL RULES AND REGULATIONS

6. Cleanroom Access  To enter the cleanroom you need to complete: General Lab Safety Training (DEHS) Hazardous Waste Safety Training (DEHS) Cleanroom Access Training followed by a Safety Quiz you MUST pass Users must provide speedtype number and complete a process review with a staff member. Process Specific Training (Provided by Cleanroom Staff) ○ You must train for specific processes and machines – instructions on how to set up training sessions are on page 8 in the manual.

7. General Rules and Restrictions  All rules must be observed at all times by all persons qualified to work in the cleanroom.  Remember that not following these rules can result in the loss of cleanroom privileges.  Complete text of rules are in the manual on pages 9 and 10.

8. Cleanroom Safety  Only qualified individuals may enter the cleanroom.  All equipment usage must be logged.  In order to use equipment you must be a certified user.  Review Material Safety Data Sheets (MSDS) for any chemicals you may use.  Check any item used (especially gowns and PPE) for contamination before use.  Do not bring in nonessential items.  NEVER WORK ALONE!

9. Cleanroom Integrity  No food, drinks, smoking, chewing gum or pencils.  Only necessary items are allowed in the cleanroom.  Avoid sneezing, coughing, whistling and singing.  Do not enter the HPM room or Service Corridor.  Clean up after yourself.

10. Appropriate Attire  No makeup.  No contacts.  Full length pants must be worn at all times.  Only closed-toed shoes are allowed.  No muddy shoes.  Bunny suits, safety glasses, boots, hair nets, mustache/beard nets and gloves must be worn at all times.

11. Hours of Operation Open 24/7 Staff hours are 9AM-5PM, Monday through Friday (with the exception of UofL holidays). Remember, you can never work alone!

12. Laundry Wash gowns once every two weeks. Wash gowns when contaminated. To have gown washed, place in laundry hamper in gowning room and fill out log on wall. Make sure to note any contamination.

13. PREVENTATIVE SAFETY TRAINING

14. Emergency Equipment and Locations in the Cleanroom Section 10 of the manual (page 17) lists important safety equipment that you will need to be familiar with. This equipment will be referenced throughout the rest of the presentation and you will be required to label most of it on your quiz.

15. Personal Protective Equipment  For personal protection against chemical spills.  These are chemical resistant – not chemical proof.  Always wear when working with chemicals.  Always inspect Personal Protective Equipment before and after use. Check for holes, stains and other indicators of contamination.  Do not wear PPE outside of the wet bay.  Complete directions in Section 11 of manual (page 21)

16. How to Wear Personal Protective Equipment 1. Inspect Apron2. Tie Apron3. Use 10% IPA in Water on towel to clean Face Shield 4. Put on Face Shield 5. Adjust Face Shield 6. Put on Chemical Resistant Gloves over apron sleeves OR 7. Double Glove with Nitrile Gloves.

17. How to Wear Personal Protective Equipment 1. Check gloves, apron and face shield for contamination. If not easily cleaned, throw away. 2. Wash gloves 3. Dry Gloves using a towel 4. Remove and Hang Face Shield 5. Remove and Hang Apron

18. Material Safety Data Sheets  Also known as MSDS’s.  You must thoroughly review a chemical’s MSDS before using that chemical.  Copies of MSDS’s for all chemicals can be found in: The Gowning Room The Loading Dock The Back Corridor Online

19. Rules for Working with Chemicals (pages 28-29)  You must read the MSDS before working with a chemical.  Know if the chemicals you use must be used in glass or plastic beakers.  Always wear appropriate protective equipment.  Never wear PPE outside of the wet bench area.  Always stand when working at wet benches.  Only work with chemicals in their designated areas.

20. Rules for Working with Chemicals  Assume any liquid is potentially dangerous.  Only use dedicated tanks/baths as they are designated.  Uncap only one bottle at a time. Pour chemicals slowly.  Keep gloves dry and clean.  When using hot plates: Only heat Pyrex beakers Constantly monitor temperature

21. Rules for Working with Chemicals When mixing chemicals: Pour acids into water. Do not mix acids and solvents. Do not mix halogenated solvents with non- halogenated solvents. Label all mixtures. Do not leave chemicals unattended. Do not pour chemicals back into storage bottle.

22. Rules for Working with Chemicals Always put the cap back on securely. Define new bottles, including Hazardous Waste bottles. Clean up your work area. Place empty bottles on the bottom shelf of the chemical cabinet it came from. Make sure all PPE is clean and dry before returning it to storage areas.

23. RESPONDING TO SAFETY INCIDENTS

24. Responding to Safety Incidents The following slides detail basic steps to follow in case of emergency An emergency poster is placed in the gowning room for quick reference. Two important phone numbers: 911 – Emergency Services 502-589-8222 – Poison Control

25. Fire Alarm  You Hear: The Fire Alarm  You See: Flashing White Lights  You DO: Evacuate the Building Immediately – Meet in Lutz Hall Lobby  Warn others as you exit  Do not attempt to de-gown  Do not try to finish an experiment or figure out why an alarm is sounding

26. HAZMAT Alarm  You Hear: The HAZMAT Alarm  You See: Flashing Blue Lights  You DO: Evacuate the Building Immediately – Meet in Lutz Hall Lobby  Warn others as you exit  Do not attempt to de-gown  Do not try to finish an experiment or figure out why the alarm is sounding

27. Carbon Dioxide Alarm  You Hear: The Carbon Dioxide Alarm  You See: Flashing White Lights from 1 or more benches  You DO: Evacuate Building Immediately – Meet in Lutz Hall Lobby  Warn others as you exit  Do not attempt to de-gown  Do not try to finish an experiment or figure out why the alarm is sounding.

28. Fire and HAZMAT Alarm  You Hear: The Fire Alarm AND the HAZMAT Alarm  You See: Flashing White and Blue Lights  You DO: Evacuate the Building Immediately – Meet in Lutz Hall Lobby  Warn others as you exit  Do not attempt to de-gown  Do not try to finish an experiment or figure out why the alarm is sounding

29. Minor Chemical Spill on a Person  Minor chemical spills are not life threatening, are not a health hazard and do not pose long-term health risks or risks of disfigurement.  Call Poison Control and Staff.  Place affected skin under running water for 15 minutes.  Remove any contaminated clothes or jewelry.  If ER trip is needed, call 911 and bring MSDS with you.

30. Major Chemical Spill on a Person  Major spills are immediately life threatening, a health hazard or pose long-term health risks or risk of disfigurement.  Call 911 and demand an ambulance.  Get under a running safety shower.  Remove all contaminated garments and jewelry.  Bring MSDS with you to the ER.

31. Flammable Solids on Skin Brush off as much as possible. Treat as small spill on skin. Check the MSDS before using any chemical – some flammable solids react with water!

32. Chemical Splash in Eyes Have a buddy call 911. If you are wearing contacts – take them out! Flush eyes in eyewash fountain for 15 minutes. Go to ER and bring MSDS.

33. Safety Buddies If you see someone who is in distress, you automatically become their safety buddy. Call 911. Be sure you are wearing proper protective apparel before helping the victim. Take MSDS to the ER.

34. First Aid for Other (Minor) Incidents  Chemical Inhalation Check area for safety. Close open containers. Move victim to safety. Call 911. Resuscitate with rescue breathing if necessary.  Chemical Ingestion Go to ER or call Poison Control.

35. First Aid for Other (Minor) Incidents  Thermal Burn Immerse burned area in cold water. Cover with sterile dressing. Call 911 if severe.  Bleeding Place clean pad and pressure on the wound. If excessive get medical attention.  Clothing Fire Douse victim with safety shower or smother with fire blanket.  CALL 911!

36. Hydrofluoric Acid (HF)  HF is a very dangerous chemical.  If contact occurs: Immediately rinse affected skin with water. Remove all jewelry or clothing exposed to HF. Have a buddy CALL 911. After washing for 5 minutes, do not dry the skin. Cover entire affected area with calcium gluconate gel (Calgonate). Continuously apply fresh gel while on the way to the ER and while waiting.

37. HF in Eyes Have a buddy call 911 and request an ambulance Flush eyes at eyewash station for 5 minutes After 5 minutes, switch to calcium gluconate (Calgonate) eyewash Continue flushing until told to stop in ER Get MSDS

38. Chemical Spills  Simple Spill: Does not spread rapidly Is not toxic Does not endanger people or property (except by direct contact) Does not endanger environment outside of building Less than ½ a gallon of liquid  Large Spill Spreads rapidly Toxic Endangers people or property Endangers outside environment More than ½ a gallon of liquid Any amount of HF

39. Simple Spill Check self for contamination. Check bench and floor for contamination. Label the spill. Contact cleanroom staff for supervision of cleanup.

40. Large Spill Check self for contamination. Push HAZMAT alarm. Evacuate the cleanroom. Call 911.

41. Mercury Spills If a Mercury Lamp explodes: Activate the HAZMAT alarm and evacuate the cleanroom. Warn others as you exit. Do not attempt to de-gown. Do not try to finish an experiment. Call 911 and alert them if anyone is injured.

42. More Information  We encourage you to read through the manual again at your own pace.  Also, check out our website for more information: http://www.louisville.edu/research/cleanroom http://www.louisville.edu/research/cleanroom You can access MSDS’s, SOP’s, copies of the safety quiz and safety manual, request equipment training and new chemicals and schedule use of equipment.

43. So… When do I get my access? Take the cleanroom tour. Complete the cleanroom layout part of the safety quiz. Read your manual on your own. Complete your safety quiz and return it to 232. Make sure to provide your advisor’s speedtype on your quiz! Go through a process review with a staff member.