2. Photosynthesis Chapter 10

4. Photosynthesis

5. (a) Plants (b) Multicellular alga (c) Unicellular eukaryotes (d) Cyanobacteria (e)Purple sulfur bacteria 40 μm 1 μm 10 μm

6. Experimental history Jan Baptista van Helmont Plants made their own food Joseph Priestly Plants “restored” the air

7. Experimental history Jan Ingenhousz Sun’s energy split CO 2 Carbon & Oxygen Oxygen was released into air Carbon combined with water Make carbohydrates

8. Experimental history Fredrick Forest Blackman 1. Initial “light” reactions are independent of temperature 2. Second set of “dark” reactions are independent of light Dependent on CO 2 concentrations & temperature Enzymes involved in light-independent reactions

9. Experimental history C.B. van Neil Looked at light in photosynthesis Studied photosynthesis in Bacteria

10. C.B. van Neil CO 2 + 2H 2 S  (CH 2 O) + H 2 O + 2S CO 2 + 2H 2 A  (CH 2 O) + H 2 O + A 2 CO 2 + 2H 2 O  (CH 2 O) + H 2 O + O 2

11. C.B. van Neil O 2 produce from plant photosynthesis comes from splitting water Not carbon dioxide Carbon Fixation: Uses electrons & H + from splitting water Reduces carbon dioxide into organic molecules (simple sugars). Light-independent reaction

12. CO 2 + 2H 2 O  (CH 2 O) + H 2 O + O 2

13. Photosynthesis Organisms capture energy from sunlight Build food molecules Rich in chemical energy 6CO 2 + 12H 2 O ⇨ C 6 H 12 O 6 + 6H 2 O + 6O 2

14. Photosynthesis Captures only 1% of sun’s energy Provides energy for life Source of energy when life began

15. Photosynthesis Photon: Packets of energy UV light photons have greater energy than visible light UV light has shorter wavelengths

16. Photosynthesis Visible light Purple shorter wavelengths More energetic photons Red longer wavelengths Less energetic photons

17. Spectrum

18. Visible light Gamma rays X-rays UVInfrared Micro- waves Radio waves 380450 500550600650700750nm Shorter wavelength Higher energy Lower energy Longer wavelength 10 − 5 3 nm 1 nm 3 nm10 6 nm10 9 (10nm) 1 m 10 3 m

19. Absorption Spectrums Photon of energy strikes a molecule Absorbed by the molecule or lost as heat Depends on energy in photon (wavelength) Depends on atom’s available energy levels Specific for each molecule

20. Leaf structure Stoma (Stomata) opening on leaf Exchange of gases. Chloroplasts Mesophyll layer of leaf

21. Chloroplasts Thylakoids: Internal membranes of chloroplasts Grana: Stacks of thylakoids Chlorophyll: Green pigment Captures light for photosynthesis Membranes of thylakoids

22. Chloroplasts Stroma: Semi-liquid substance Surrounds thylakoids Contain enzymes Make organic molecules from carbon dioxide

23. Chloroplasts

24. Fig. 10-3b 1 µm Thylakoid space Chloroplast Granum Intermembrane space Inner membrane Outer membrane Stroma Thylakoid

25. Figure 10.4 Stroma Granum Thylakoid space Outer membrane Intermembrane space Inner membrane 20 μm Stomata Chloroplast Mesophyll cell 1 μm Mesophyll Chloroplasts Vein Leaf cross section CO 2 O2O2

26. Pigments Molecules Absorb energy in visible range Chlorophylls & Carotenoids Chlorophyll a & b Absorb photons in the blue-violet & red light

27. Pigments Chlorophyll a main pigment of photosynthesis Converts light energy to chemical energy Chlorophyll b & carotenoids are accessory pigments Capture light energy at different wavelengths

28. Pigments

29. Chlorophyll b Carotenoids Chlorophyll a

30. Chlorophyll structure Located in thylakoid membranes A porphyrin ring with a Mg in center Hydrocarbon tail Photons are absorbed by the ring Absorbs photons very effectively Excites electrons in the ring

31. Chlorophyll structure

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33. Carotenoids Two carbon rings attached by a carbon chain Not as efficient as the Chlorophylls Beta carotene (helps eyes) Found in carrots and yellow veggies

34. Photosystem Cluster of photosynthetic pigments Membrane of thylakoids (surface) Each pigment captures light energy Photosystem then gathers energy Energy makes ATP & NADPH

35. Photosystems Chlorophyll a molecules Accessory pigments (chlorophyll b & carotenoids) Associated proteins

36. Photosystems Consists of 2 components 1. Antenna (light gathering) complex 2. Reaction center

37. Photosystem 1. Antenna complex Gathers photons from sun Web of Chlorophyll a molecules Held by proteins in membrane Accessory pigments carotenoids Energy is passed along the pigments to reaction center

38. Photosystems 2. Reaction centers 2 special chlorophyll a molecules Accept the energy Chlorophyll a than passes the energized electron to an acceptor Acceptor is reduced (quinone)

39. Photosystem

40. Fig. 10-12 THYLAKOID SPACE (INTERIOR OF THYLAKOID) STROMA e–e– Pigment molecules Photon Transfer of energy Special pair of chlorophyll a molecules Thylakoid membrane Photosystem Primary electron acceptor Reaction-center complex Light-harvesting complexes

41. (a) How a photosystem harvests light(b) Structure of a photosystem Chlorophyll STROMA THYLA- KOID SPACE Protein subunits Thylakoid membrane Pigment molecules Primary electron acceptor Reaction- center complex STROMA Photosystem Light- harvesting complexes Photon Transfer of energy Special pair of chloro- phyll a molecules THYLAKOID SPACE (INTERIOR OF THYLAKOID) Thylakoid membrane e−e−

42. 2 photosystems Photosystem I (older) Absorbs energy at 700 nm wavelength Generates NADPH Photosystem II (newer) Absorbs energy at 680 nm wavelength Splits water (releases oxygen) Generates ATP 2 systems work together to absorb more energy

43. NADP + Nicotinamide Adenine Dinucleotide Phosphate Coenzyme Electron carrier Reduced during light-dependent reactions Used later to reduce carbon Carbon dioxide forms organic molecules Photosynthesis is a redox reaction

44. Photophosphorylation Addition of phosphate group to ADP Light energy

45. Photosynthesis Occurs in 3 stages 1. Capturing energy from sun 2. Energy makes ATP Reducing power in NADPH 3. ATP & NADPH Power synthesis of organic molecules

46. Photosynthesis Light dependent reactions First 2 steps of photosynthesis Presence of light Light-independent reactions Formation of organic molecules Calvin cycle Can occur +/- light

47. Photosynthesis 1. Chloroplasts 2. Light-dependent reactions Sun’s energy makes NADPH & ATP 3. Light-independent reactions ATP & NADPH CO 2 into organic molecules

48. Light Fig. 10-5-4 H2OH2O Chloroplast Light Reactions NADP + P ADP i + ATP NADPH O2O2 Calvin Cycle CO 2 [CH 2 O] (sugar)

50. Photosynthesis (Process) Light dependent reactions Linear electron flow Energy transfer Thylakoid membranes

51. Light dependent reactions Photosystem II (680 nm) Light is captured by pigments Excites an electron (unstable) Energy is transferred to reaction center (special chlorophyll) Passes excited electron to an acceptor molecule

52. Light dependent reactions PS II is oxidized Water splits (enzyme) Water donates an electron to chlorophyll Reduces PS II Oxygen (O 2 ) is released with 2 protons (H + )

53. Light dependent reactions Electron is transported to PS I (700 nm) Electron is passed along proteins in the membrane (ETC) Protons are transported across the membrane Protons flow back across the membrane & through ATP synthase Generate ATP

54. Light dependent reactions At the same time PS I received light energy Excites an electron Primary acceptor accepts the electron PS I is excited Electron from PS II is passed to PS I Reduces the PS I

55. Light dependent reactions PS I excited electron is passed to a second ETC Ferredoxin protein NADP + reductase catalyzes the transfer of the electron to NADP + Makes NADPH

56. Pigment molecules Light P680 e–e– Primary acceptor 2 1 e–e– e–e– 2 H + O2O2 + 3 H2OH2O 1/21/2 4 Pq Pc Cytochrome complex Electron transport chain 5 ATP Photosystem I (PS I) Light Primary acceptor e–e– P700 6 Fd Electron transport chain NADP + reductase NADP + + H + NADPH 8 7 e–e– e–e– 6 Fig. 10-13-5 Photosystem II (PS II)

57. Fig. 10-UN1 CO 2 NADP + reductase Photosystem II H2OH2O O2O2 ATP Pc Cytochrome complex Primary acceptor Primary acceptor Photosystem I NADP + + H + Fd NADPH Electron transport chain Electron transport chain O2O2 H2OH2O Pq

58. Enhancement effect

60. Fig. 10-17 Light Fd Cytochrome complex ADP + i H+H+ ATP P synthase To Calvin Cycle STROMA (low H + concentration) Thylakoid membrane THYLAKOID SPACE (high H + concentration) STROMA (low H + concentration) Photosystem II Photosystem I 4 H + Pq Pc Light NADP + reductase NADP + + H + NADPH +2 H + H2OH2O O2O2 e–e– e–e– 1/21/2 1 2 3

61. Fig. 10-16 Key Mitochondrion Chloroplast CHLOROPLAST STRUCTURE MITOCHONDRION STRUCTURE Intermembrane space Inner membrane Electron transport chain H+H+ Diffusion Matrix Higher [H + ] Lower [H + ] Stroma ATP synthase ADP + P i H+H+ ATP Thylakoid space Thylakoid membrane

62. Photosystems Noncyclic photophosphorylation 2 systems work in series Produce NADPH & ATP Replaces electrons from splitting water System II (splits water)works first then I (NADPH)

63. Photosystems When more ATP is needed Plant changes direction Electron used to make NADPH in PS I is directed to make ATP

66. Calvin Cycle Named for Melvin Calvin Cyclic because it regenerates it’s starting material C 3 photosynthesis First organic compound has 3 carbons

67. Calvin cycle Combines CO 2 to make sugar Using energy from ATP Using reducing power from NADPH Occurs in stroma of chloroplast

68. Calvin Cycle Consists of three parts 1. Fixation of carbon dioxide 2. Reduction-forms G3P (glyceraldehyde 3-phosphate) 3. Regeneration of RuBP (ribulose 1, 5 bisphosphate)

69. Calvin Cycle 3 cycles 3 CO 2 molecules 1 molecule of G3P 6 NADPH 9 ATP

70. Fixation of carbon CO 2 combines with Ribulose 1, 5 bisphosphate (RuBP) Temporary 6 carbon intermediate Splits-forms 2- three carbon molecules 3-phosphoglycerate (PGA) Large enzyme that catalyses reaction (Rubisco) Ribulose bisphosphate carboxylase/oxygenase

71. Reduction Phosphate is added to 3- phosphoglycerate 1,3 Bisphosphoglycerate NADPH reduces the molecule Glyceraldehyde 3-phosphate (G3P)

72. Regeneration 5 molecules of G3P are rearranged to make 3 RuBP Uses 3 more ATP

73. Fig. 10-18-3 Ribulose bisphosphate (RuBP) 3-Phosphoglycerate Short-lived intermediate Phase 1: Carbon fixation (Entering one at a time) Rubisco Input CO 2 P 3 6 3 3 P P P P ATP 6 6 ADP P P 6 1,3-Bisphosphoglycerate 6 P P 6 6 6 NADP + NADPH i Phase 2: Reduction Glyceraldehyde-3-phosphate (G3P) 1 P Output G3P (a sugar) Glucose and other organic compounds Calvin Cycle 3 3 ADP ATP 5 P Phase 3: Regeneration of the CO 2 acceptor (RuBP) G3P

74. Fig. 10-UN2 Regeneration of CO 2 acceptor 1 G3P (3C) Reduction Carbon fixation 3 CO 2 Calvin Cycle 6  3C 5  3C 3  5C

75. Calvin Cycle 3 CO 2 enter cycle & combine with RuBP Generates 3 molecules more of RuBP & one G3P (glyceraldehyde 3- phosphate) G3P can be made into glucose & other sugars

76. Calvin Cycle Enzyme mediated 5 of these enzymes need light to be more efficient Net reaction 3CO 2 + 9 ATP + 6NADPH ⇨ G3P + 8P i + 9ADP + 6NADP+

77. G3P Converted to fructose 6-phosphate (reverse of glycolysis) Made into sucrose Happens in cytoplasm Intense photosynthesis G3P levels rise so much some is converted to starch

78. Fig. 10-21 Light Reactions: Photosystem II Electron transport chain Photosystem I Electron transport chain CO 2 NADP + ADP P i + RuBP 3-Phosphoglycerate Calvin Cycle G3P ATP NADPH Starch (storage) Sucrose (export) Chloroplast Light H2OH2O O2O2

79. Summary Light reactions Thylakoids Use Sun’s energy Make ATP & NADPH Split water make oxygen

80. Summary Dark reactions Stroma Use ATP & NADPH Make G3P Regenerate ADP, Inorganic P, and NADP

81. O2O2 CO 2 H2OH2O Sucrose (export) H2OH2O Light LIGHT REACTIONS: Photosystem II Electron transport chain Photosystem I Electron transport chain Chloroplast NADP  ADP  P i NADPH ATP RuBP G3P CALVIN CYCLE Starch (storage) 3-Phosphoglycerate Sucrose (export) O2O2 H2OH2O Mesophyll cell CO 2

82. Flow of Genetic Information in the Cell: DNA → RNA → Protein (Chapters 5–7) Movement Across Cell Membranes (Chapter 7) Energy Transformations in the Cell: Photosynthesis and Cellular Respiration (Chapters 8–10) DNA mRNA Nucleus Nuclear pore Protein Ribosome mRNA Protein in vesicle Rough endoplasmic reticulum (ER) Vesicle forming Golgi apparatus Protein Plasma membrane Cell wall Photosynthesis in chloroplast Organic molecules Transport pump Cellular respiration in mitochondrion ATP CO 2 H2OH2O H2OH2O O2O2 O2O2 5 4 3 2 1 7 8 Vacuole 9 10 11 6 MAKE CONNECTIONS The Working Cell

83. Photorespiration Rubisco oxidizes RuBP (starting molecules of Calvin cycle) Oxygen is incorporated into RuBP Undergoes reactions that release CO 2 CO 2 & O 2 compete for same sight on the enzyme Under conditions greater than the optimal 25 0 C this process occurs more readily

84. Photorespiration Hot Stoma in leaf close to avoid loosing water Carbon dioxide cannot come in Oxygen builds up inside Carbon dioxide is released G3P is not produced

85. C 4 Photosynthesis Process to avoid loosing carbon dioxide Plant fixes carbon dioxide into a 4 carbon molecule (oxaloacetate) PEP carboxylase (enzyme) Oxaloacetate is converted to malate Then taken to stroma for Calvin cycle Sugarcane and corn

86. Mesophyll cell Bundle- sheath cell Photo- synthetic cells of C 4 plant leaf Vein (vascular tissue) C 4 leaf anatomy Stoma The C 4 pathway Mesophyll cell PEP carboxylase Oxaloacetate (4C) Malate (4C) Pyruvate (3C) CO 2 ADP PEP (3C) ATP CO 2 Calvin Cycle Bundle- sheath cell Sugar Vascular tissue

87. CAM Process to prevent loss of CO 2 Plants in dry hot regions (cacti) Reverse what most plants do Open stoma at night Allows CO 2 to come in & water to leave Close them during the day.

88. CAM Carbon fix CO 2 at night into 4 carbon chains (organic acids) Use the Calvin cycle during the day.

89. Fig. 10-20 CO 2 Sugarcane Mesophyll cell CO 2 C4C4 Bundle- sheath cell Organic acids release CO 2 to Calvin cycle CO 2 incorporated into four-carbon organic acids (carbon fixation) Pineapple Night Day CAM Sugar Calvin Cycle Calvin Cycle Organic acid (a) Spatial separation of steps (b) Temporal separation of steps CO 2 1 2