1. I.Microscopes A. Magnification – to increase the size of an image of an object B. Resolution – how clear the image appears…the more magnified, the more blurry the image (light bends) C. Types of Microscopes 1. Light microscope – light shines through the object a. simple – one lens b. compound – two lenses (what we have) 2. Electron microscope – bounces a beam of electrons off of an object coated in gold. Only see surfaces of objects Honors Biology Chapter 4

2. Pictures of Electron Microscope at Miami

3. Cat Flea

4. D. Parts and Function of a compound light microscope 1. Base – supports microscope 2. Light – shines through the object 3. Stage – where the slide sits when viewing 4. Eyepiece lens – lens closest to eye (mag.10X) 5. Arm – connects the eyepiece to the base 6. Objective lens – lens closest to object a. 4X b. 10X c. 40X d. 100X 7. Diaphragm – adjusts the amount of light shining through the object (disk vs. iris) 8. Nosepiece – rotates the objective lenses 9. Coarse adjustment – brings object into the field of view 10. Fine adjustment – focuses object, increases resolution

5. Eyepiece Arm Coarse Adjustment Fine Adjustment Nosepiece Objective lens Stage Diaphragm Light Base