1. Microscopy Observing microorganisms

2. Light microscopy – any microscope that uses visible light

3. Magnification vs. Resolution Magnification-Something that has been magnified; an enlarged representation, image, or model. Resolution- ability for lens to distinguish fine detail and structure

4. Total Magnification = “Magnification of specific objective lens” x “ocular lens magnification” Objective x ocular = magnification 41040 1010100 4010400 100101000

5. Resolution Ability of the lens to distinguish two points a specified distance apart Ex: If the microscope has a resolving power of 0.4 nm, it can distinguish two points if they are least 0.4 nm apart Light has long wavelength and cannot resolve structures smaller than about 0.2 micrometer

6. *To achieve high magnification with good resolution, the objective lens must be small *Specimen must contrast with substance in which they are suspended (medium) *Reflective index (light bending ability) must be changed. We do this with staining *Light rays will be refracted with the two materials and will bend * Oil immersion has same effect as increasing objective lens