1. Protein Synthesis DNA is transcribed into Messenger RNA. Messenger RNA is translated into Protein

2. Goals Compare the structure of RNA with that of DNA Summarize the process of transcription Relate the role of codons to the sequence of amino acids after translation

3. Are Genes and Enzymes Related? In 1909,Archibald Garrod “suggested” that genes dictate phenotypes through enzymes.Garrod's One Gene-One Enzyme Hypothesis (click the link)Garrod's One Gene-One Enzyme Hypothesis In 1936 Beadle & Ephrussi supported Archibald’s ideas with research concerning fruit fly eye color. http://www.genetics.org/cgi/reprint/22/1/ 76.pdf http://www.genetics.org/cgi/reprint/22/1/ 76.pdf

4. One Gene – One Enzyme 1941 George Beadle and Edward Tatum experimented with the bread mold, Neurospora crasa. They showed that a single gene mutation could result in a single enzyme deficiency.Genome News Network - Timeline: 1941. (Click the Link to Learn More.)Genome News Network - Timeline: 1941

5. Current Hypothesis One gene directs the synthesis of a single polypeptide. ONE GENE = ONE POLYPEPTIDE

6. Central Dogma

7. Transcription

8. Transcription RNA molecules-Nucleic acids made of linked nucleotides Different How? Single stranded, not double Ribose instead of Deoxyribose Contain Uracil instead of Thymine

9. Transcription Transcription-When the instructions for making a protein are transferred from a gene to an RNA molecule Translation-When instructions on an RNA molecule are read and coded as proteins

11. The Stages of Transcription 1)RNA polymerase binds to the promoter and unwinds the DNA and begins RNA synthesis. 2)RNA polymerase adds RNA nucleotides to the template strand of DNA in a 5’ to 3’ direction. 3)RNA polymerase transcribes the terminator sequence which signals the process to end. The RNA is then released.

12. Transcription The DNA molecule unzips One side of the DNA acts as a template for making RNA At the end, the RNA is unzipped from the DNA Portions called introns are taken out. Portions called exons are spliced together

13. http://www.youtube.com/watch?v=ztPkv 7wc3yU&feature=related&safety_mode =true&persist_safety_mode=1&safe=act ivehttp://www.youtube.com/watch?v=ztPkv 7wc3yU&feature=related&safety_mode =true&persist_safety_mode=1&safe=act ive

14. Transcription to translation RNA is transcribed on the DNA in the nucleus mRNA is translated in the cytoplasm

15. Ribosome The ribosome is made of two subparts The ribosome becomes attached to the mRNA After a tRNA has given up its amino acid it breaks off and goes back into the cytoplasm to find another amino acid

16. Messenger RNA The long form of RNA is called messenger RNA (mRNA) The mRNA leaves the nucleus and goes into the cytoplasm The mRNA lies along an endoplasmic reticulum

17. mRNA mRNA has thousands of nitrogenous bases The bases are divided into 3 base words called codons The mRNA becomes attached to a ribosome The ribosome moves along the mRNA and allows tRNA to join onto the codons

18. Translation Process by which the proteins in our body are coded for

19. Translation Genetic Code-codes that give us the proteins in our body, this is based on three letters How many three letter words can be made from the letters A, R, E, and T

21. A tRNA molecule consists of a strand of about 80 nucleotides that folds back on itself to form a three-dimensional structure. It includes a loop containing the anticodon and an attachment site at the 3’ end for an amino acid.

22. Transfer RNA (tRNA) tRNA molecules find a specific amino acid within the cytoplasm tRNA molecules have an anticodon on their ends. tRNA takes its amino acid to a specific codon on the mRNA

23. Each ribosome has a binding site for mRNA and three binding sites for tRNA molecules. The P site holds the tRNA carrying the growing polypeptide chain. The A site carries the tRNA with the next amino acid. Discharged tRNAs leave the ribosome at the E site.

24. Initiation brings together mRNA, a tRNA with the first amino acid, and the two ribosomal subunits. First, a small ribosomal subunit binds with mRNA and a special initiator tRNA, which carries methionine and attaches to the start codon. Initiation factors bring in the large subunit such that the initiator tRNA occupies the P site.

25. The three steps of elongation continue codon by codon to add amino acids until the polypeptide chain is completed.

26. Termination occurs when one of the three stop codons reaches the A site.

27. Translation Steps….#1 mRNA leaves the nucleus and enters cytoplasm Methionine is always the first amino acid Start codon is AUG tRNA molecule binds methionine to AUG codon

28. Step #2 Codon in A site of ribosome is ready to receive the next tRNA tRNA with the complementary anticodon arrives and binds to the codon with the right amino acid

29. Step #3 A and P site are holding tRNA molecules Enzymes bind the adjacent amino acids together

30. Step #4 tRNA goes to the E site, where it detaches to go get another amino acid

31. Step #5 tRNA in the A site moves over and fills the empty P site Because the A site is open another tRNA brings an amino acid and bonds to the growing protein

32. Step #6 tRNA goes to E site and exits

33. Step #7 Steps 2 through 6 are repeated until a stop codon is reached Stop codons-UAG, UAA, UGA Newly made protein is released into the cell

34. http://www.youtube.com/watch?v=- zb6r1MMTkc&NR=1&safety_mode=true &persist_safety_mode=1&safe=active

35. http://www.youtube.com/watch?v=41_N e5mS2ls&safety_mode=true&persist_sa fety_mode=1&safe=active

36. Gene Regulation and Structure Goals Describe how the lac operon is turned on or off Summarize the role of transcription factors in regulation eukaryotic gene expression Describe how eukaryotic genes are organized

37. Regulation Both prokaryotic and eukaryotic cells are able to control which genes are expressed and which are not

38. Regulation Operator-The piece of DNA that overlaps the promoter site and serves as the on-off switch

39. Regulation In Bacteria Genes that code for enzymes involved in the same function, their promoter site and operator is called the OPERON Lac Operon-controls the metabolism of lactose

40. Regulation Repressor-a protein that binds to an operator and physically blocks RNA polymerase from binding to a promoter site