1. Nutritional Requirements of Tissue culture 1.Salts. 2. Carbohydrate. 3. Amino acids. 4. Vitamins. Basic Principles of Tissue culture 1.Environment ( Temperature,hydrogen ion concentration, essential metabolites, matrix ). 2. Prevention of contamination, Removal of harmful Metabolites. Applications: 1.Embryoloy ( IVF, Stem cells & Cloning). 2.Virology ( Vaccines). 3.Genetic ( Human genome, karyotype of diseases, etc.

2. Tissue culture Kinds of techniques: 1.Culture in clot of cover slip. 2.Culture on a watch glass. 3.Culture on petri dishes. 4.Culture on ordinary test tubes, Erlenmeyer flask, round bottomed flasks.

4. Steps of IVF In Vitro Fertilization (IVF) provides an alternative method of achieving a pregnancy for couples who have tried and have been unsuccessful with other fertility treatment. steps are as follows: 1.Retrieval of the mature egg. 2.Sperm collection, fertilization, and embryo development. 3.Transfer of the developing embryo(s) into the uterus. 4.Implantation and growth of the embryo(s).

5. Information for all prospective IVF patients : All female partners entering the program be tested for antibodies against Rubella. Alcohol, and cigarette smoking should be eliminated during the IVF cycle. Sitting in hot tubs and saunas is not recommended during the IVF cycle. Do not begin any new exercise, sport, or intense athletic training during the IVF cycle. All women attempting conception should be taking a specific prenatal vitamin. No other supplements should be taken unless specifically prescribed.

6. IVF Application of tissue Culture