1. Lab 2: Organelles, Proteins, and SDS-PAGE
Dr. Sarah VanVickle-Chavez
BIO 2960 Laboratory
Spring 2013

2. Overview
Today we will load a gel and compare your gel to the sample gel. Each person will load one lane on the gels in the center of each bench.
While gels are running, we will do the PyMOL computer lab as a group demo. You should have Lab Report 1a complete before we leave today.
You can use your books and notes, but there is a brief computer tutorial on the NSLC website to help you with Lab Report 1b.
Next week, there are three reports due at the beginning of class
1a = proteins (PyMOL demo in class)
1b = cell organelles (use your book/notes for this one)
2 = SDS-PAGE

3. 1. Protein Electrophoresis
SDS-PAGE stands for sodium dodecyl sulfate polyacrylamide gel electrophoresis

4. SDS vs Native Gel
How do you “see” your proteins?

5. PyMOL
Remember: Amino acids have different properties based on the R-group
GAVLIMPFW = nonpolar
STNQYC = uncharged, polar
KRHDE = charged, polar

6. Alpha helix

7. Beta Sheet