1. S Y P H I L I S

2. Causative Organism – Treponema pallidum Characteristics of Treponema pallidum
Spirochaetes or spiral organisms, they are motile, slender.
Do not grow in artificial medium (some treponema are part of the normal oral flora e.g. T.denticulc)
Cannot be seen by light microscopy because they are very thin (0.15 μm), long μm.
Note: Does not stain with gram stain

3. Sensitive to penicillin
Causative Organism – Treponema pallidum Characteristics of Treponema pallidum (Continued)
Can be seen by
Dark field microscopy, by
Phase contract technique
Can be stained by
Silver impregnation
Fluorescent antibody technique
Sensitive to penicillin
They can be propagated by inoculation in rabbits in testes and anterior chamber of eye.

5. Non-venereal disease by direct contact A sexually transmitted disease
Causative Organism – Treponema pallidum Characteristics of Treponema pallidum (Continued)
Treponema
Pathogenic
Non-pathogenic
Oral commensals
Pathogenic
Non-venereal disease by direct contact
T. Denticula T.macrodentium T.microdentium
T.pallidum
Syphilus
T.Pertenue T. carateum T.pallidum
A sexually transmitted disease
Yaws
Pinta
Bejal

6. Mode of Transmission Direct sexual contact (90 – 96%)
Blood transfusion
Via placenta from infected pregnant mother faetus causes congenital syphilis.
Contact accidental contact E.g. Medical personnel.
Source of T. pallidum: Primary and secondary syphilis lesions.

7. Treponema pallidum is the causative organism of syphilis.
Syphilis can be Acquired
Congenital
Clinical Features of Syphilis / Symptoms and signs of Acquired syphil
Syphilis is a sexually transmitted disease / a venereal disease
Incubation Period: 10 – 90 days (average – 21 days)

8. 3 Stage of Syphilis:
1- Primary syphilis: Primary chancre develops after 2-10 weeks a well defined indurated painless ulcer mainly on the genitalia (90%) and extra-genital on Lips (5-10%). In female, chancre occurs in the cervix. The chancre is painless and exudate is formed in the centre. This fluid is highly infectious and examination by dark field microscopes shows Spirochaetes. There is regional lymphadenopathy.
Primary chancre heals spontaneously without treatment within 3-8 weeks.
Primary syphilis is highly infectious.
Serological tests for Syphilis are positive in 80% cases.

12. 2- Secondary Syphilis: After 6-8 weeks of primary chancre:
Desseminated secondary stage develops. Muco-cutaneous lesion occurs e.g. Skin rash, mucasal ulcers, condylomata on genitalia, Lymphadenopathy, fever headache malaise, alopecia.
Secondary syphilis is highly infectious
Snal – truck mucosal ulcers in the mouth Hepatitis, glomerulonephritis, periostitis, iridocyclitis, choroidoretinitis, arthritis.
Serological tests for syphilis becomes almost uniformly positive.
Secondary Stage may follows by the following:
a) Cured spontaneously
b) Early latent
c) Late Latent
d) Tertiary stage

17. Latent Stage:
After the secondary syphilis symptoms subsides, the disease enters a latent stage.
After about 2 years, the syphilis is NOT normally infectious, except from mother to the foetus.

18. 3- Tertiary Syphilis:
After 2-20 yrs, tertiary stage develops produces
Gummatous Lesions in perforation of the palate (Roof of the mouth) which interferes with speech
Skin
Bone
Joints Charcoat’s joints
Cardiovascular System
E.g. (a) Aortic aneurism
(b) Aortic valve incompetence

19. Tertiary stage is not infectious.
Central Nervous System: Neurosyphilis
E.g. a) Tabes dorsalis
b) General paralysis of insane
c) Meningovascular sympilis
Tertiary stage is not infectious.

21. Congenital Syphilis: most distressing and dangerous form of Syphilis.
Early Congenital syphilis:
a) Skin: rash maculopapular rash
b) Mucosal Lesion: Mucocutaneous lesions
c) Hepatospleenomegaly
d) Lymphadenopathy
Late Congenital Syphilis:
a) Hutchinson’s teeth (Notching of the incisor teeth)
b) Mulberry molars, Moon’s molars
c) Sadle nose
d) Sabre shin (tibia)
d) Interstitial keralitis, blindness

22. f) Deafness
g) Bone sclerosis, Arthritis
h) Juvenile general paralysis of insane
i) Damage of Mental development and other neurological
symptoms.
j) Stillbirths

26. Laboratory of Syphilis
1- Dark ground microscopy to demonstrate, Spirochaetes T. pallidum in fluid or exudate from lesions of primary and secondary syphilis.
a) Primary Syphilis exudate from chancre
b) Secondary syphilis mucous path exudate taken for dark ground microscopy.
Direct immunofluorescent microscopy can be used.

29. Laboratory of Syphilis (Continued)
2- Serological tests for Syphilis for all stages
A. Non-specific Tests
B. Specific Tests
(for non-treponemal)
(for non-treponemal or reagin antibody)
(Ag used is Treponemal antigen)
(Ag used is cardiolipin)
1- VDRL (Venereal Disease
Research Laboratory)
1- FTA – ABS Test (Florescent
Treponemal Antibody Absorption)
2- TPHA (Treponema pallidum
Haemagglutination.
3- TPI (Treponema pallidum immobilization)
2- RPR (Rapid Plasma reagin)
3- WR (Wasserman Reaction)
Congenital Syphilis Baby’s blood IgM –FTA-ABS TEST

31. Serological Tests for Syphilis (With Interpretation)
Stage of disease VDRL TPHA FTA-ABS
No Past or Present Infec.
No Past /present Infection
Primary (Early)
(Active Syphilis).
(Active Syphilis).
Primary (Late) Or
(Active Syphilis) in secondary syphilis.
Secondary + Tertiary
Latent Or
Treated syphilis
(had infection before)

32. Serological Tests for Syphilis (With Interpretation) (Continued)
Stage of disease VDRL TPHA FTA-ABS
Biological false positive
(No Infection by T. pallidum)
Congenital syphilis*
* Early primary syphilis – FTA – ABS
Positive
* After successful treatment – VDRL Negative
Note: FTA – ABS after successful treatment remains positive for life
But FTA – ABS + TPHA
Remain positive
* V.D.R.L is used to see efficacy (effect) of treatment . After successful treatment V.D.R.L. becomes negative.

33. Serological Tests for Syphilis (With Interpretation) (Continued)
Treatment of syphilis :
* Penicillin is the drug of choice
* Penicillin for 15 days.
Primary:
Secondary and Tertiary syphilis :
Penicillin for 21 days usually followed by 10 injection at weekly intervals.
Note:
Spirochetes are spiral motile bacteria. Their motility is due to contractile axial fibers run along the bacterial cell.

34. Spirochetes (spiral bacteria)
Borrelia
Treponema
Leptospira
Borrelia recurrentis
Borrelia vincenti
Pathogenic genera of spirochaetes are : * Borrelia, * Leptaspira, * Treponema
Borrelia recurrentis
Source : Rodents
Disease :
Epidemic Louse borne relapsing fever
Endemic Louse borne relapsing fever
Treatment:
Tetracycline

35. Spirochetes (Spiral bacteria) (Continued)
Borrelia vincenti : Gram –ve irregular spiral bacteria
Culture : Strict anaerobic bacteria, difficult to culture.
* Serum enriched media used Anaerobic culture
Laboratory diagnosis : Mainly by Microscopic Examination of Gram stained smear only
Disease : Borrelia vincenti and Fusobacterium species
together produce:
Vincent’s angina (Pharynigitis) or
Acute necrotizing ulcerative gingivitis
Gingivo - Stomatitis
Sore Throat
Treatment :
Penicillin or Metronidazole
Oral hygiene

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