1. NAHLN- A State and Federal Partnership to Safeguard Animal Health NAHLN Update Barbara Martin and Elizabeth Lautner U.S. Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services National Veterinary Services Laboratories Secretary’s Advisory Committee on Animal Health July 22, 2011 July 2011 1

2. NAHLN- A State and Federal Partnership to Safeguard Animal Health  Previously covered NAHLN background and capabilities  Update on 2011 Activities  Budget Impacts 2 July 2011 Presentation Overview

3. NAHLN- A State and Federal Partnership to Safeguard Animal Health July 2011  Brief History of NAHLN  Mission and Purposes of NAHLN  Partnership  Founding principles  Structure and laboratory designations  Current capabilities and their application The National Animal Health Laboratory Network 3

4. NAHLN- A State and Federal Partnership to Safeguard Animal Health July 2011  NAHLN Methods Technical Working Group  Standardized, rapid diagnostic techniques AI, END, BSE, CWD, FMD, CSF, PRV, SIV, Scrapie, VS  Secure communications and reporting system via NAHLN IT System  Train the trainer program  Quality standards and proficiency testing program  Laboratory review and approval process Current Capabilities 4

5. NAHLN- A State and Federal Partnership to Safeguard Animal Health Update on 2011 Activities July 2011 5

6. NAHLN- A State and Federal Partnership to Safeguard Animal Health 2011 Quality Management System Trainings  QMS training Developed in partnership with AAVLD Accreditation Committee Modified from August 2010 initial delivery Interactive learning environment AAVLD, ISO 17025/ OIE standard o Document control, records, internal auditing, management review, corrective actions, root cause analysis Wet lab – application of skills  Development of QMS distance learning modules  Initial module developed  National and international feedback  Collaborative training with PPQ’s National Plant Disease Network– April 2011 26 participants  Collaborative training with International Services– August 2011 10 participants from NAHLN laboratories 16 expected participants from 5 foreign countries (Kazakhstan, Ukraine, Russia, Kenya, and Tanzania) July 2011 6

7. NAHLN- A State and Federal Partnership to Safeguard Animal Health Diagnostic Development NVSL/FADDL and NAHLN  Completed Negative Cohorts for FMD, ASF, Rinderpest– April 2011 11 NAHLN labs participated ~ 6000 FMD, 1100 ASF and 1350 RP samples tested Assay validation, result sharing and communication protocols  Current projects: Collaboration between with NVSL, DHS, FAZD, NAHLN labs: o RT-PCR for FMD in milk o Optimization of RNA extraction o Negative cohort o FMD Penside test o Negative cohort Collaboration between NVSL, DHS and NAHLN labs: Deployment of FMD Serology to NAHLN labs Negative cohort Negative cohorts for Lumpy Skin Disease and Contagious Bovine Pleuopneumonia 7 July 2011

8. NAHLN- A State and Federal Partnership to Safeguard Animal Health Secure Communication and Reporting System Laboratory Capacity Estimation Program  Collaboration between NAHLN/FAZD/AAVLD  Software tool for evaluating/monitoring NAHLN capacity Provide daily testing and surge capacities for individual and overall NAHLN diagnostic testing Prioritize resources Manage a large number of diagnostic tests simultaneously  Currently in pilot phase in 11 NAHLN labs  Upcoming NAHLN-wide release mid-August 2011  Applicability for other networks 8 July 2011

9. NAHLN- A State and Federal Partnership to Safeguard Animal Health Secure Communications and Reporting System NAHLN Portal  Modules in development Secure mechanism to electronically comment on and release SOPs Laboratory directory including information on physical space, personnel and equipment Mechanism to monitor assay performance Mechanism to submit and receive PT results Mechanism to train others in the validation process Work Group and collaboration space to post documents and schedule meetings and calls Mechanism to schedule meetings and training  Currently in pilot phase  User acceptance testing by NAHLN labs in Fall 2011 9 July2011

10. NAHLN- A State and Federal Partnership to Safeguard Animal Health Scenario Testing FMD Tabletop Exercise Series 10  Inspired by the 2007 HPAI Exercise Series  5 part series VS Policy-level workshop 16 table top exercises NVSL table top exercises Follow-up policy exercise Overall report to be released Fall 2011  CONOPS – DHS - FAZD July 2011

11. NAHLN- A State and Federal Partnership to Safeguard Animal Health NAHLN Coordinating Council 11 July2011  Established 2009 with State and Federal representation  Regular meetings  Face to face as possible and monthly conference calls  Currently reviewing possible options for enhanced laboratory network structures as well as the NAHLN strategic plan  Will obtain input from state, Federal and industry stakeholders prior to finalization

12. NAHLN- A State and Federal Partnership to Safeguard Animal Health NAHLN Budget July 2011 12

13. NAHLN- A State and Federal Partnership to Safeguard Animal Health NAHLN Budget FY10 NIFA  $4.4M/year through Food and Ag Defense Initiative  Funding supports NAHLN lab infrastructure 12 Core and 16 Member labs Personnel – technical, QA, and IT as well as equipment APHIS  $6.5 M/year through AHMS and Vet Diagnostics  Funding supports laboratory review process, personnel, IT infrastructure, training, surveillance, reference materials, proficiency testing, maintenance of equipment, and infrastructure support of QMS and IT systems in 12 Member labs 13 July2011

14. NAHLN- A State and Federal Partnership to Safeguard Animal Health NAHLN Budget FY 11  NIFA funding 39% cut to FADI Spread evenly across labs  APHIS funding $6.5 M 14 July2011

15. NAHLN- A State and Federal Partnership to Safeguard Animal Health NAHLN Budget FY 12  NIFA funding House of Representatives zeroed out FADI AAVLD, AVMA, and AAVMC worked together Amendment to restore $4.4 M to Integrated Programs Senate to address agriculture appropriations  APHIS funding CWD program funding Wild bird avian influenza surveillance reduction 15 July2011

16. NAHLN- A State and Federal Partnership to Safeguard Animal Health July 2011 Conclusions  Partnerships are key to the success of NAHLN  Numerous NAHLN accomplishments in 2011  Upcoming activities for 2012 to continue to increase preparedness and functionality of network 16

17. NAHLN- A State and Federal Partnership to Safeguard Animal Health Questions/Discussion? July 2011 17

18. NAHLN- A State and Federal Partnership to Safeguard Animal Health July 2011 NAHLN Information  Brochure and Information Cards  Posters  Website:http://www.aphis.usda.gov/animal_health/nahlnhttp://www.aphis.usda.gov/animal_health/nahln  Contact information: E-mail nahln@aphis.usda.gov or phone 515-337-7731nahln@aphis.usda.gov 18