1. Vrn1_T4 (AS_ITA_21_HQ910536) genomic sequence vs. corresponding fragment from T. aestivum Vrn-A1: F primer Exon 3Intron 3 Exon 4 Intron 4 Supplement 4: alignments for putative Vrn1 clone used for RFLP mapping (November, 2011):

2. Intron 4Exon 5 Intron 5 R primer Intron 5 Exon 6 79% identity

3. AS_ITA_21_HQ910536 spliced sequence* vs. A. strigosa Fruitful-like cDNA and spliced T. aestivum Vrn-A1 sequence: * Two sequencing errors corrected to match oat Vrn1 consensus sequence

4. AS_ITA_21_HQ910536 translated sequence vs. Z. mays TF15 protein sequence and translated A. strigosa Fruitful-like and T. aestivum Vrn-A1 sequences: K-box motif