1. Viruses Living or Not ???????

2. Viruses Virus – Latin for “_Poison__” Virus – Latin for “_Poison__” TMV - 1930 TMV - 1930

3. Characteristics of Viruses Science of Viruses – Virology! Scientist that studies viruses is called a virologist! Among the smallest biological particles (not cells) that are capable of causing harm in living organisms. Among the smallest biological particles (not cells) that are capable of causing harm in living organisms. Virus particles are called virions Virus particles are called virions 5000 influenza viruses can fit inside the head of a pin! 5000 influenza viruses can fit inside the head of a pin!

4. Studying the “T4-Bacteriophage Also known as: “eaters of bacteria” have a head and tail region – (serve as the principle experimental object in studying viruses) Also known as: “eaters of bacteria” have a head and tail region – (serve as the principle experimental object in studying viruses) Check out: http://www.cellsalive.com/ph age.htm http://www.cellsalive.com/ph age.htm

5. Not Considered Living A virus is not a bacteria, fungus, protist, plant or animal. A virus is not a bacteria, fungus, protist, plant or animal. They do not carry out cellular functions. They do not carry out cellular functions. They do not grow or undergo division They do not grow or undergo division A virus can not replicate on their own – they need to infect a HOST and use the hosts organelles and enzymes to do this! A virus can not replicate on their own – they need to infect a HOST and use the hosts organelles and enzymes to do this!

6. What are Viruses Made of? Viruses are composed of nucleic acid, proteins, and sometimes, lipids. They are not very complex! Viruses are composed of nucleic acid, proteins, and sometimes, lipids. They are not very complex! The Nucleic acid core, which can be either DNA or RNA, encodes the genetic information to make virus copies. The Nucleic acid core, which can be either DNA or RNA, encodes the genetic information to make virus copies. The nucleic acid is surrounded by a protective protein coat, called a capsid. These are called “naked” viruses. The nucleic acid is surrounded by a protective protein coat, called a capsid. These are called “naked” viruses. An outer membranous layer, called an envelope, made of lipid and protein, surrounds the capsid in some viruses…..these ones are called “enveloped viruses” An outer membranous layer, called an envelope, made of lipid and protein, surrounds the capsid in some viruses…..these ones are called “enveloped viruses” The capsid accounts for 95% of the total virus, and gives the virus a particular shape The capsid accounts for 95% of the total virus, and gives the virus a particular shape

7. Virus Structure Nucleic acid Envelope Capsid

8. How are Viruses Classified? Genetic Material Genetic Material DNA or DNA or RNA RNA But not both But not both Capsid Structure Capsid Structure  Helical (rod shaped)  Polyhedral (spherical)  Complex Presence or absence of a membranous envelope surrounding the capsid Presence or absence of a membranous envelope surrounding the capsid Helical Marburg virus Polyhedral SV40 virus Complex bacteriophage

9. VIRUSES & CELLS CharacteristicVIRUSCELL Structure DNA or RNA core, capsid Reproduction Only within a host Genetic Code DNA or RNA Growth/DvpmtNO EnergyNO Response to Environment No Change over time Yes Mem. e.Organelles - no Psotn yes yes yes yes.o

10. History of Viruses Invasions, wars, and immigration can introduce viral diseases to different populations Invasions, wars, and immigration can introduce viral diseases to different populations Some viral infections have had major global effects: Some viral infections have had major global effects: Influenza type A is believed to be responsible for global flu outbreaks in 1918, 1957 and 1968 Influenza type A is believed to be responsible for global flu outbreaks in 1918, 1957 and 1968 16th century Spanish explorers brought small pox, measles and influenza viruses to the New World 16th century Spanish explorers brought small pox, measles and influenza viruses to the New World

11. Viruses - Disease Viral infections generally behave in 2 ways: 1. Viruses reproduce and cause disease immediately (lytic or active) 2. Viruses integrate into the host cell (lysogenic or dormant)

12. Viral Replication – Lytic Cycle Infection See clip on desktop!!

13. LYSOGENIC CYCLE See clip on desktop!!!

14. Viral Replication – Lysogenic Cycle Infection The host cell makes copies of the virus indefinitely The host cell makes copies of the virus indefinitely The virus integrates its DNA into the DNA of the host cell, and the viral genetic information replicates along with the host cell’s DNA. The virus integrates its DNA into the DNA of the host cell, and the viral genetic information replicates along with the host cell’s DNA. Unlike lytic viruses, lysogenic viruses DO NOT lyse the host cell right away. (it can remain inactive for a period of time) Unlike lytic viruses, lysogenic viruses DO NOT lyse the host cell right away. (it can remain inactive for a period of time) The viral DNA embedded in the host’s DNA is called a prophage and it can remain in the host cell for many… generations before becoming activated The viral DNA embedded in the host’s DNA is called a prophage and it can remain in the host cell for many… generations before becoming activated

15. What organisms and Host cells do Viruses Infect? INFECTION BY A VIRUS: Viruses infect bacteria, plants, animals and other living organisms in order to reproduce. Viruses infect bacteria, plants, animals and other living organisms in order to reproduce. Narrow host range. Ex: smallpox is only capable of infecting humans and no other species. Narrow host range. Ex: smallpox is only capable of infecting humans and no other species. Viruses are cell specific. Ex: viruses that cause respiratory disease usually infect cells only in the respiratory tract. Viruses are cell specific. Ex: viruses that cause respiratory disease usually infect cells only in the respiratory tract. Determined by a lock-and-key fit between the virus and the receptor (proteins) on the surface of a host cell. Determined by a lock-and-key fit between the virus and the receptor (proteins) on the surface of a host cell.

16. HIV, for example, only will enter cells that have a surface protein molecule called CD4. These molecules are found only on white blood cells. Thus, HIV will only infect white blood cells and not lung cells or other cell types. Sometimes, a virus can mutate and change its host range. This appears to be happening with the avian flu virus (influenza A/H5N1) currently circulating. At first, the flu virus could infect only birds, but changes in the virus genome have allowed it to infect humans. So now, its host range has expanded. The change in host range of a virus can be one factor in the emergence of a new disease. Host Range

17. What Diseases are Caused by Viruses? Infectious diseases Infectious diseases RNA viruses (retroviruses)DNA viruses AIDSRespiratory Disease Influenza (flu) Smallpox Common coldChickenpox PolioMononucleosis MeaslesCold Sores Tumor Viruses – cause Cancer ex: HPV Tumor Viruses – cause Cancer ex: HPV

18. How Can Viral Diseases Be Prevented and Treated? Good hygiene Good hygiene Avoid contact with contaminated food, water, fecal material or body fluids. Avoid contact with contaminated food, water, fecal material or body fluids. Wash hands frequently. Wash hands frequently. Vaccines Vaccines Stimulate natural defenses with in the body. Stimulate natural defenses with in the body. Contain a component of or a weakened or killed virus particles. Contain a component of or a weakened or killed virus particles. Are developed for many once common illnesses such as smallpox, polio, mumps, chicken pox. Are developed for many once common illnesses such as smallpox, polio, mumps, chicken pox. Not available for all viruses. Not available for all viruses. Anti-viral drugs (but not antibiotics) Anti-viral drugs (but not antibiotics) Available for only a few viruses. Available for only a few viruses. Inhibit some virus development and/or relieve symptoms. Inhibit some virus development and/or relieve symptoms.

19. How Do New Viruses Emerge? Through mutation and evolution Through mutation and evolution By spreading from one species to a new host species By spreading from one species to a new host species By spreading from an isolated location to more widespread locations By spreading from an isolated location to more widespread locations Through the detection of previously unrecognized viruses Through the detection of previously unrecognized viruses

20. HSV1 vs HSV2

23. Human Papilloma Virus – 14yo boy

24. HPV virus – genital warts

26. Polio Virus Six countries (Afghanistan, Egypt, India, Niger, Nigeria, and Pakistan) still have polio circulating

28. VIRUS MINI QUIZ 1. THE PRIMARY FUNCTION OF ALL VIRUSES IS TO REPLICATE!!

29. MINI QUIZ… 2. WHAT IS THE TYPICAL/COMMON VIRUS THAT STUDENTS STUDY WHEN LEARNING ABOUT VIRUSES? T4 Bacteriophage

30. MINI QUIZ… 3. IN ORDER FOR A VIRUS TO REPLICATE IT NEEDS TO BE IN A HOST CELL

31. MINI QUIZ… 4. VIRAL REPRODUCTION THAT KILLS THE HOST CELL IS CALLED THE Lytic CYCLE!

32. MINI QUIZ… 5. WHEN VIRAL DNA IS EMBEDDED INTO A HOST CELL’S DNA IT FORMS A PROPHAGE

33. MINI QUIZ… 6. ANOTHER NAME FOR INFLUENZA IS FLU VIRUS

34. MINI QUIZ… 7. 3 WAYS VIRUSES CAN BE TREATED/PREVENTED ARE…. 1. Hygiene 2. Vaccines 3. AntiVirals

35. MINI QUIZ… 8. SCIENTISTS THAT STUDY VIRUSES ARE CALLED VIROLOGISTS

36. MINI QUIZ… 9. 3 WAYS THAT VIRUSES CAN BE TRANSMITTED ARE…. -- droplets in the air (e.g. cold, flu, tuberculosis) -- via food or water (e.g. Salmonella, food poisoning) -- via mosquitoes, ticks (e.g. malaria, West Nile virus, Lyme disease) -- physical contact (e.g. pinkeye, herpes, chickenpox, sexually transmitted diseases)

37. MINI QUIZ… 10. A VIRUS THAT HAS ONLY A CAPSID SURROUNDING IT’S GENETIC INFORMATION AND NO ENVELOPE IS CALLED A NAKED VIRUS.