Presentation on theme: "Biological Integration of Spatial and Temporal Localization of genes in C. Elegans Cesar A. Hidalgo R. CCNR University of Notre Dame & CCSB, DFCI, Harvard."— Presentation transcript:
Biological Integration of Spatial and Temporal Localization of genes in C. Elegans Cesar A. Hidalgo R. CCNR University of Notre Dame & CCSB, DFCI, Harvard University
mRNA A brief review of genetics Gene Promoter Transcription Factor Polymerase Upstream Downstream Genes are not always on
The Combination of Genes that are ON and OFF determines the phenotype
Systems Biology RNAi MicroArray GFP-Localization PPI Genetic Interactions Regulatory Networks -How are these connected? -When are they expressed? (Condition) -When are they expressed? (Time) -Where are they expressed? -How do they turn on? (Regulatory Networks) How does the organism work…?
Who is connected with whom..? Protein-Protein Interactions
How do they turn on? Regulatory interactions…? (Positive) Gene1 Gene2 Polymerase
How do they turn on? Regulatory interactions…? (Negative) Gene1 Gene2 Polymerase
What happens when we remove one..? RNAi MicroArray GFP-Localization PPI Genetic Interactions Regulatory Networks Knock outs Gene1 Gene2 Gene3 Any difference….?
What happens when we remove two..? Genetic Interactions..? Gene1Gene2 Gene3
Under which conditions are they expressed? Microarray
Where are they expressed? GFP Gene Promoter Transcription Factor Polymerase Upstream Downstream Gene Encoding GFP
Where is the field at? Collecting data and developing methods..? PPI: Cite… Rual et. Al. Ito. El al. Uetz. et. Al. Steltz et. Al. RNAi: Microarray. S. Kim. et. Al. Coverage is still very low compared to what is expected to be out there….
Where is it going..? PPI Co-Expression Localization
The Localizome A high throughput way to determine spatio-temporal expression patterns in c. elegans.
COPAS Biosort-Profiler Union Biometrica Fluorescence The Localizome (The Data) Profile Individuals
Summary Reliable localization data can be produced in a high throughput with the profiler technology. This data can be used potentially to identify the sub-networks involved in different organs and in different developmental stages. So far we see that the overlap between interacting partners tends to be partial.