1. A new method to address cross-reactivity of antibodies
Zestern analysis
A new method to address cross-reactivity of antibodies

2. Cross-reactivity of antibodies
One antibody, one protein (antigen) is the basis of immunoblot analysis
One antibody, two or more proteins (antigens) is the reality.
Majority of antibodies for research purpose actually recognize more than one protein (Cross-reactivity)*.
*531 out of 22,000 antibodies are found without cross-reactivity
Schwenk et al. Mol. & Cell Proteomics 9: ,2010.
cross-reactivity of antibodies
reflected in Western blot analysis
Antibody w/o
Cross-reactivity
Antibody w/
Cross-reactivity

3. Traditional methods to address cross-reactivity of antibodies
Western blot analysis: Gel electrophoresis
Dot blot analysis
ELISA
Protein microarrays
The number of samples can be analyzed are
limited due to gel electrophoresis step
Extensive screening to identify
antibodies without cross-reactivity
The number of suitable antibodies are limited.
Insufficient coverage
high developmental cost

4. Introducing Zestern analysis

5. Basic of Zestern analysis
Excessive antigen
Excessive partial antigen
Synthesized epitope peptide
Synthesized epitope repeat Peptide
Antibody
Antigen
Nitrocellulose
membrane
Epitope

6. How Zestern analysis achieves specificity

7. Advantages over traditional immunoblot analysis
Eliminates gel electrophoresis step in Western
Blot analysis.
Increases number of antibodies available for Dot blot, ELISA and protein microarray analysis.
Increases the coverage of proteins for Dot blot, ELISA and protein microarray analyses.
Serves as foundation for high throughput protein analysis.
Provides basis for automation of immunoblot analysis.

8. Further Info.
Zestern Biotechnique LLC owns US patent for Zestern analysis.
PCT application has been filed by Zestern Biotechnique LLC.
For further inquiry, contact:
Dr. Jiandi Zhang
(001)