1. Protein arrays LEAPS technology An array of 110 different antibodies incubated with various levels of the fluorescently labelled cognate antigens in a serum background. (Courtesy Dr Brian Haab, The Van Andel Research Institute, Grand Rapids, MI, USA)

2. Introduction  Detect proteins  Monitor their expression levels  Investigate protein interactions and functions

3. Production of a Protein Array Coated surface Robotic protein application Arrayed proteins Incubation with fluorescent labelled sample Scan

4. Defining characteristics Protein arrays are solid-phase ligand binding assay systems using immobilised proteins on surfaces which include glass, membranes, microtiter wells, mass spectrometer plates, and beads or other particles. highly parallel (multiplexed) and often miniaturised (microarrays, protein chips).

5.  Rapid  Automatable  Capable of high sensitivity  Economical on reagents  Giving an abundance of data for a single experiment. Advantages

6. Areas of application 1. Diagnostics: detection of antigens and antibodies in blood samples; profiling of sera to discover new disease markers; environment and food monitoring. 2. Proteomics: protein expression profiling; organ and disease specific arrays. 3. Isolation of individual members from display libraries for further expression or manipulation: selection of antibodies and protein scaffolds from phage or ribosome display libraries for use in capture arrays. 4. Protein functional analysis: protein-protein interactions; ligand-binding properties of receptors; enzyme activities; antibody cross reactivity and specificity, epitope mapping.

7. Protein sources  Cell-based expression systems for recombinant proteins  Purification from natural sources  Production in vitro by cell-free translation systems  Synthetic methods for peptides Many of these methods can be automated for high throughput production. For capture arrays and protein function analysis, it is important that proteins should be correctly folded and functional; this is not always the case, e.g. where recombinant proteins are extracted from bacteria under denaturing conditions. Nevertheless, arrays of denatured proteins are useful in screening antibodies for cross-reactivity, identifying autoantibodies and selecting ligand binding proteins.

8. Formats and surfaces Miniaturisation of familiar immunoassay methods such as ELISA and dot blotting. Commonly used physical supports include glass slides, silicon, microwells, nitrocellulose or PVDF membranes, and magnetic and other microbeads.

9. CompanyArchitectures Gyros CD centrifugation devices based on developments in microfluidics Biotrovemicrochannels in a plate Zyomyx3D posts on a silicon surface Bio-Radcolour coding for microbeads Quantum Dotssemiconductor nanocrystals Smartbeadsbarcoding for beads Nanoplex Technologies multimetal microrods BioArray Solutions beads assembled into planar arrays on semiconductor chips

10. Protein immobilisation considerations  Variables in immobilisation of proteins include: The coupling reagent The nature of the surface being coupled to  The properties of a good protein array support surface are: Should be chemically stable before and after the coupling procedures, Allow good spot morphology Display minimal nonspecific binding Not contribute a background in detection systems Be compatible with different detection systems.

11. The immobilisation method used should be: Reproducible Applicable to proteins of different properties (size, hydrophilic, hydrophobic) Amenable to high throughput and automation Compatible with retention of fully functional protein activity Protein immobilisation considerations

13. CompanyDetection From DNA arraydifferent fluorophores (e.g. Cy-3, Cy-5) PerkinElmer Lifesciences tyramide signal amplification (TSA) Zeptosensplanar waveguide technology Bio-Radphycoerythrin as label [Luminex] Molecular Stagingrolling circle DNA amplification HTS Biosystems, Intrinsic Bioprobes surface plasmon resonance Ciphergen, Intrinsic Bioprobes mass spectrometry Genicon Sciences BioForce Laboratories resonance light scattering atomic force microscopy

18. Large-scale protein arrays Incubation of the slide with anti-RGSHis antibody reveals the recombinant human proteins Detected with a Cy3-labeled secondary antibody. Protein array containing 192 purified human proteins on glass.

19. Challenges and Bottlenecks