1. Pacific Northwest Ballast Water Research Facility Andrea Copping Marine Research Operations Pacific Northwest National Laboratory Sequim, WA December 2007

2. Today….Today…. PNW Ballast Water RDTE Location at PNNL Marine Sciences Lab Virtual tour of PNNL Sequim Labs Explain PNNL to you

3. RDTE Facility Pacific Northwest Ballast Water Treatment Facility Pacific Northwest National Laboratory, Sequim WA Sponsored by National Oceanic and Atmospheric Administration and United States Fish and Wildlife Service

4. Northwest RDTE at PNNL Sequim $1.25M Award by NOAA to build RDTE Design and construction: October ‘07-January ‘09 Only commercial test bed on west coast; one of 2 in country NRL Key West doing development work Capabilities for testing at Sequim (ecotox, species ecology, chemistry, etc.)

5. Rationale for Developing RDTE Ballast Water Facility at Sequim High quality abundant seawater and freshwater Reliable permitted wastewater treatment system Adequate land to add facilities Established facilities management and O&M Secure facility Scientific and engineering staff, experience in protocol development, testing, ballast water testing

6. Marine Research Operations at Sequim  1965 - Battelle purchased Bugge Clam Cannery site  1973 - Beach wet laboratory & offices  1982 - Uplands analytical chemistry labs and offices  1992 - Twenty new offices at beach  2004 - New 44 office building completed  2006 - New Biotechnology Laboratory 197319651982 Today Tomorrow

7. Protecting people & the environment Coastal assessment & restoration Marine biotechnology/ ecotoxicology Coastal Security PNNL’s MRO Focused in 3 Areas Developing new products & energy sources Securing our ports, coastlines & waterways

8. Virtual Tour of the MRO

9. Sea Water Intake  Two independent intakes Class AA seawater from Sequim Bay  Four separate distribution systems Seawater and freshwater to testing facilities  Ambient seawater (9°C to 11°C) at a rate of 250 gpm  Seawater supplied to labs at 4°C to 38°C  Up to 20 gpm freshwater from uncontaminated groundwater from 11°C to 30°C

10. Waste Water Treatment Facility  40,000 gal ground holding tank comprised of four separate cells  Removal of particulates - sedimentation and filtration  Disinfection of biologic agents - breakdown of organics using ozonation  Removal of organics and disinfection byproducts via granular activated carbon adsorption Treated Wastewater Discharge – permitted through the NPDES process.

11. Aquatic Wet Laboratory Facilities Two wet laboratory facilities ~5300 sq ft Marine, estuarine, and freshwater bioassay testing Over 40 species commonly tested Tiered approach to environmental characterization Natural resource damage assessment (NRDA) Remedial investigation / feasibility studies Risk assessment Good laboratory practice (GLP) testing

12. Analytical and Biotech Support  BSL 1 and BSL 2 Laboratories  Flow Cytometer & Microscope (FlowCAM) continuously monitors of plankton abundance  Pulsed Amplitude Modulation (PAM) chlorophyll fluorometer  Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) detection of large, intact biomolecules  Digital imaging microscope

13. Marine / Estuarine Chemistry Understanding the source and fate of chemicals in aquatic systems Standard Water Quality Parameters Metals Ultra-Low (part-per-trillion) Detection Limits in Seawater Arsenic Speciation & Methylmercury in Water, Sediment, and Tissue Organics Fuel Fingerprinting Semipermeable Membrane Devices Endocrine Disrupting Chemicals Methods Development

14. Sequim Marine Research Operations Operating since 1966 Located on 140 acres of uplands and tidelands at mouth of Sequim Bay, Strait of Juan de Fuca, 90 miles from ocean 11,000 sq ft of office space 6000 sq. ft of analytical and general purpose laboratories 1500 sq ft biotechnology laboratory 7700 sq. ft. of wet/bioassay and support laboratory space Uplands facility ten analytical laboratories and 35 offices Beach area is comprised of four principal buildings office building with 44 offices, a bioassay laboratory building two climate-controlled bioassay/culturing labs two isolation rooms and supporting analytical labs biotechnology laboratory seawater pumping and wastewater treatment facilities

15. HOW WILL TREATMENT TECHNOLOGIES BE EVALUATED?

16. Project Example  EPA’s Endocrine Disruptor Screening Program  $34 M research program  Development of tools for screening & testing chemicals that have potential endocrine disruptor effects  No standardized tests for evaluating these compounds exist  Final product: EPA-certified protocol Protocol /Method Development, Evaluation, Standardization and Validation

17. Expert Staff to meet the needs of the RDTE The MRO has a total of 85 scientists and engineers in: biology chemistry physical oceanography biological oceanography ecological sciences environmental sciences ecotoxicology fisheries marine invertebrate carcinologist coastal engineering chemical engineering civil engineering marine engineering electrical engineering Team Partners

18. PNNL in the Northwest Seattle Sequim Richland Portland, OR Battelle operates PNNL for the US Department of Energy

19. Battelle’s purposes stem from the 1925 will of steel industrialist Gordon Battelle Non-profit charitable trust – began operations in 1929 75 years of scientific research and development Creative activities of a scientific nature Application and licensing of inventions, discoveries, and developments Major business is R&D, laboratory operations, and commercial ventures $2.9 billion in annual R&D 19,000 employees worldwide

20. Continuous Operation of PNNL Since 1965 Battelle won the contract in 1964 and began operating PNL in 1965 Original mission focused on nuclear technology FY05 PNNL Business Facts Approximately $726 million in business volume 4,200 staff $288 million in purchases for goods and services

21. Battelle Now Manages or Co-Manages 5 National Labs National Renewable Energy Laboratory (with MRI) Brookhaven National Laboratory (with SUNY– Stony Brook) Oak Ridge National Laboratory (with the University of Tennessee) More than $2 billion 15,000 staff Idaho National Laboratory Pacific Northwest National Laboratory Richland and Sequim, Washington

22. Thank you for opportunity to be here We would like to invite you to visit us at Sequim MRO Andrea Copping andrea.copping@pnl.gov