1. Syllabus CBE3014 – Genetic Engineering (유전공학)
Lecture: Mon 13:30 – 14:45
Fri 12:00 – 13:15
Lecture Room: AS301
Instructor: Hyuncheol Kim
R417,
Teaching Assistant: Ji-Hwang Lee
R417,
Grading Policy: Absolute Evaluation
Mid-Term Exam %
Final Exam %
Assignment %

2. Syllabus
Textbook:
Terry A. Brown, Gene Clonging & DNA Analysis (An Introduction), 6th Edition, Wiley-Blackwell Publishing, 2010

3. Chapter 1. Why Gene Cloning and DNA Analysis are Important

4. What is the genetic engineering?
Genetic engineering is a scientific development that involves the artificial manipulation of an organism’s genes by using techniques such as molecular cloning and transformation in order to alter their nature and structure. Many of these transformations are achieved by manipulation of an organism’s DNA, which effectively is the code inscribed in every cell to determine how it will function.

5. Definition of Genetic engineering
Scientific alteration of the structure of genetic material in a living organism.
It involves the production and use of recombinant DNA and has been employed
to create bacteria that synthesize insulin and other human proteins.

6. The Applications of Genetic Engineering

7. The Applications of Genetic Engineering
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Genetech (source : E. coli)

8. Genetic Engineering in Medicine

9. Genetic Engineering in Agriculture
Engineering resistant corn. Following the insertion of a gene from the bacteria Bacillus thuringiensis, corn becomes resistant to corn borer infection. This allows farmers to use less insecticides.

10. Genetic Engineering in Energy
Metabolic Engineering

11. Genetic Engineering in Energy

12. The Applications of Genetic Engineering
Gene Delivery Vector
Gene Therapy

13. The Applications of Genetic Engineering
GM rice delivers antibodies against deadly rotavirus
- Researchers have added an antibody to fight rotavirus into the rice genome.
Researchers engineered the rice, called MucoRice-ARP1, by adding an antibody to fight rotavirus originally found in llamas in the rice genome.
Rotavirus is the leading cause of severe diarrhea in young children and infants, killing more than 520,000 people each year, according to the WHO. More than 85 per cent of those deaths occur in impoverished countries in Africa and Asia.
The rice could be used to complement vaccinations to protect children when they are at their most vulnerable to rotavirus, say the researchers.

14. ZMAPP: EXPERIMENTAL EBOLA TREATMENT PRODUCED IN GENETICALLY MODIFIED PLANTS

15. What is gene cloning ?

16. What is gene cloning ?
A fragment of DNA, containing the gene to be cloned, is inserted into a circular DNA molecule called a vector, to produce a recombinant DNA molecule. (Chapter 2, 3, 4)
The vector transports the gene into a host cell, which is usually a bacterium, although other types of living cell can be used. (Chapter 5, 7)
Within the host cell the vector multiplies, producing numerous identical copies, not only of itself but also of the gene that it carries. (Chapter 5)
When the host cell divides, copies of the recombinant DNA molecule are passed to the progeny and further vector replication takes place. (Chapter 5)
After a large number of cell divisions, a colony, or clone, of identical host cells is produced. Each cell in the clone contains one or more copies of the recombinant DNA molecules; the gene carried by the recombinant molecule is now said to be cloned. (Chapter 6, 8)

17. Vector in Molecular Biology
Plasmid Vector : an extra-chromosomal DNA molecule separate
from the choromosomal DNA which is capable of replicating
independently of the chromosomal DNA.
Cloning vector : a small piece of DNA into which a foreign DNA
fragment can be inserted.

18. Vector in Molecular Biology
Viral vectors : a tool commonly used by molecular biologists to
deliver genetic material into cells.
(e.g. Retrovitruses, Lentiviruses, Adenoviruses, Adeno-associated
viruses.)
Non-viral vectors :
- Naked DNA
- Oligonucleotides
- Lipoplexes and polyplexes
- Hybrid Methods
- Dendrimer
- etc.

19. PCR (Polymerase Chain Reaction)
Definition: A technique to amplify a single or few copies of a piece of DNA
across several orders of magnitude, generating millions or more copies of
a particular DNA sequence.
Procedure:
1. Denaturation of the template DNA
- 94oC
Annealing of the oligonucleotide
primers – 50 ~ 60oC
3. Synthesis of new DNA - 74oC

20. PCR
Summary Movie about PCR (Youtube)

21. Why gene cloning and PCR are so important
Both techniques can provide a pure sample of an individual gene, separated from all the other genes in the cell.

22. Obtaining a pure sample of a gene by cloning
Cloning allows
individual fragments
of DNA to be purified.
The problem
of selection

23. PCR can also be used to purify a gene
Gene isolation by PCR

24. The end of Chapter 1