1. Overview of Plant Stem Cell Products in Cosmetics Assoc. Prof. Dr. Ubonthip Nimmannit “Plant Stem Cell in Cosmetics” 25 August 2010

2. What are stem cells? Unprogrammed cell Differentiate into cells with specific function Advantage –Regenerative medicine Stimulate healing Extended healthy life span Reddy lab, UC Riverside Arabidopsis stem cells

3. Stem cells Self-renewal Generate differentiated cells Sablowski,TRENDS in Cell Biology, Vol.14,2004 General features of a stem-cell niche.

4. Animal and Human Stem Cells Embryonic stem cells –Pluripotent differentiation potential Adult stem cells –To maintain and repair tissue The same DNA and the same gene –Signal inside the cell –epigenetic signals tag DNA –Surrounding histone protein: regulate gene

5. Plant stem cells Shoot apical meristem Root apical meristem –Epigenetic –Totipotent http://www.microscopyu.com/staticgallery/smz1500/generalizedplantcellstem.html

6. Meristems specialized proliferation tissue – the growing tip of roots and shoots –generate new cells Dinneny and Benfey, Cell 132, 2008

7. Root meristem Quiescent center cells (4 cells) Single layer of stem cells Daughter cell (2 cells) –Cell renewal –Formation of mature tissue Dinneny and Benfey, Cell 132, 2008

8. Positioning the Root Stem Cell Niche Dinneny and Benfey, Cell 132, 2008

9. Shoot meristem Organizing center –Several layers of cells –Plant growth hormone Promote cell cycle activity Promote growth Shoot identity Dinneny and Benfey, Cell 132, 2008

10. Shoot apical meristem Branch Flower Leaves

11. Plant shoot apical meristem (SAM) and root apical meristem (RAM) SC niches. The organizing-centre cells provide signals for maintenance of the overlying SCs, which are surrounded by a TA population of cells in the meristem peripheral zone (PZ). In the RAM, the quiescent centre (QC) is the source of SC maintenance signals. Singh and Bhalla, TRENDS in Plant Science, Vol.11, 2006

12. The role of stem cells in the skin Epidermis – stratified epithelium – interfollicular epidermis Hair follicles Sebaceous glands

13. The hair bulge contains multipotent stem cells that contribute to the lineages of the epidermis, the sebaceous gland and the hair follicle Schmid et al., SÖFW-Journal | 134 | 5-2008

14. Epidermal stem cell generates transit amplifying cells that differentiate to form the stratified layers Schmid et al., SÖFW-Journal | 134 | 5-2008

15. Explant Cell Colorless mass callus Individual/ small cell clusters in liquid culture

16. Plant stem cell and their propagation in culture Sterile condition Standardized condition

17. Plant stem cell products Centella asiatica cell culture 20% Echinacea angustifolia cell culture 20% Buddleja davidii cell culture 20% Leontopodium alpinum cell culture 20% Malus domestica fruit cell culture extract

18. Claims Stimulate human stem cell growth Maintains the self renewal capacity of stem cells Increases longevity of human skin stem cells Delays senescence of essential cells Combat chronological aging Preserve the youthful look and the vitality of skin neutralize, often reversing, the effects of aging on the skin protect stem cells from death due to UV overexposure protects human skin stem cells from stress, thus decreasing wrinkles and producing younger, fresher- looking skin.

19. Plant stem cell products

20. STEM CELL ACTIVATOR with 20% Plant Stem Cells It’s all about PERCENTAGE It’s all about SYNERGY PhytoCellTec Malus Domestica (Swiss Apple) - 20% Plant Stem Cells +Phytosteron - Wild Yam and Soy Bean Sterols

21. Plant stem cell cream Lancome Paris Absolue Precious Cells Advanced Regenerating and Reconstructing Cream SPF15 Amore Pacific IOPE Plant Stem Cell Skin Renewal Eye Cream Price: $150Price: $60

22. Plant stem cell cream emerginC protocell bio-active stem cell face cream. Price: $90 Serious Skin Care Replicate Renew Plant Stem Cell Double Power Concentrate Price: $46.50

23. Plant stem cell serum Helena Rubinstein - Prodigy Powercell Elions stem cell serum Price: $120 Price: $25 La Mer The Regenerating Serum Price: $250

24. Plant stem cell : Anticellulite Collistar Intensive Anticellulite Serum Price: $60 Adonia LegTone Serum Price: $120

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