1. การทดสอบฤทธิ์ต้านปฏิกิริยา ออกซิเดชันด้วยวิธี DPPH Radical Scavenging Assay SORACHAI SAE-LIM Department of Chemistry Mahidol wittayanusorn School

2. Evalution of Antioxidation Activity The antioxidation activities of the crude extracts and pure compounds

3. The DPPH Radical Scavenging Assay AH = antioxidant compound DPPH = 1,1-diphenyl-2-picrylhydrazyl

4. The antioxidation activities of the crude extracts Evaluation of Antioxidation Activity Sample (30  l) 0.05 mM DPPH (3 mL) Spectrophotometer Absorbance at 517 nm The DPPH radical scavenging assay

5. Screening on the free radical scavenging activity of crude extracts DPPH Dichloromethane extract Acetone extract Methanolic extract

6. Free radical scavenging activity of the pure compounds 40

7. Inhibitory concentration (IC 50 ) of the crude extracts

8. The results in terms of IC 50 showed that MF 10, MF 11 and MF 14 showed strong radical scavenging activity. The activity of MF 10 and MF 14 possibly exhibited by the catechnolic moiety, ortho dihydroxyl groups (3-OH and 4-OH of B-ring in MF 10 and 6-OH and 7-OH of A-ring in MF 14) (Sata, et al., 1992). MF 10 MF 14 The mechanism of trapping radical 43

9. The mechanism of trapping radical by MF 10 (Shahidi, et al., 1992).

10. The mechanism of trapping radical by Ascrobic acid

11. Alberto, J., Selles, N., Herman, T. and Castro, T. 2002. "Isolation and Quantitative Analysis of Phenolic Antioxidants, Free Sugars and Polyols from Mango (Mangifera indica) Stem Bark Aqueous Decoction Used in Cuba as a Nutritional Supplement", Journal of Agricultural and Food Chemistry. 50, 762-766. Anjaneyulu, V., Babu, I.S. and Connolly, J.D. 1994. "29-Hydroxymangiferonic acid from Mangifera indica", Phytochemistry. 35, 1301-1303. Anjaneyulu, V., Babu, J.S. and Krishna, M.M. 1992. "Triterpenoids and Steroids from Mangifera indica", Acta Cienia Indica Chemistry. 18, 173-176. Anjaneyulu, V., Babu, I.S., Krishna, M.M. and Connolly, J.D. 1993. "3-Oxo-20(S),24 (R), Epoxy-dammarane-25b,26-diol from Mangifera indica", Phytochemistry. 32, 469-471. REFERENCES

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