1. Cell Biology Course Info and Introduction

2. What is Cell Biology? Investigation of Biological Systems –Biochemistry –Molecular Biology –Genetics/Molecular Genetics –Cell Biology Multidisciplinary Areas –Immunology –Developmental Biology –Oncology –Physiology

3. Cell Biology Study of Cellular Level Problems/Questions –Organelle structure function –Protein trafficking –Cell signaling –Cell adhesion –Cell cycle

4. Cell Biology A variety of methodologies used to study these types of questions –Molecular biological –Biochemical –Immunological –Genetic

5. Overview of This Course Introduction to Cell Structures Organelle Functions Review of Gene Expression Protein Processing & Trafficking Cell Signaling Cell Cycle

6. Categories of Cells Eukaryotic –membranous organelles nucleus, ER, Golgi, vesicles, mitochondria, plastids –cytoskeleton actin, myosin, tubulin Prokaryotic –circular chromosome

7. Cells – General Features (Eukaryotes)

8. Principles of Cell Function Genetic information –DNA in chromosome(s) –replicated during each cell division

9. Principles of Cell Function Flow of genetic information –Genetic information is transcribed into RNAs –mRNA directs the synthesis of proteins via Ribosomes (rRNA) and tRNA

10. Principles of Cell Function Self Assembly of macromolecules from smaller subunits

11. Origination of membranes – expansion of preexisting membranes following biosynthesis of necessary lipid molecules Principles of Cell Function

12. Intra- & Extracellular trafficking –Proteins and vesicles are targeted by specific signal sequences (amino acid sequences) to their appropriate subcellular locations

13. Principles of Cell Function Movement of cellular constituents –diffusion – proteins, small biomolecules, ions –pumps – ions, small biomolecules –motors – large macromolecular assemblies, organelles

14. Principles of Cell Function Cell signaling –allows cells to respond to extracellular environment –Signaling pathways Ligands – hormones, growth factors, cytokines, neurotransmitters Receptors Second messengers

15. Principles of Cell Function Control of processes by feedback mechanisms –Control propagation of signals –control biosynthesis and degradation of most substances –Cell division