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Legame cometitivo al DNA Mascheramento della superficie di attivazione Interazione con Fattori generali della trascrizione Reclutamento di complessi.

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Presentation on theme: "Legame cometitivo al DNA Mascheramento della superficie di attivazione Interazione con Fattori generali della trascrizione Reclutamento di complessi."— Presentation transcript:

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4 Legame cometitivo al DNA Mascheramento della superficie di attivazione Interazione con Fattori generali della trascrizione Reclutamento di complessi di rimodellamento della cromatina repressivi Reclutamento di istone deacetilasi

5 HELICASE/ATPase domain

6 BROMO domain binds acetylated lysines on histone tails HSA domain protein interactions actin/ARP transcription factors ATPase domain ROLE of SNF2/BRM domains SNF2 ATPase activity change nucleosome position increased regulatory protein access! change nucleosome conformation eject histone octamer displace H2A/H2B dimer Roles of SWI2/SNF2 Role in activation or repression of genes Inducible gene expression: transcription initiation transcription elongation Splicing Repair

7 Roles of ISWI nucleosome array formation chromatin assembly, replication heterochromatin formation transcriptional regulation some PolII, PolI SANT/HAND domain contacts histone tails Slide domain linker DNA contact, measures distance equal spacing of nucleosomes ATPase domain ROLE of ISWI domains

8 CHD ATPases CHD1: role in chromatin assembly; open chromatin in pluripotent cells NuRD complex also contains Me-DNA binding protein (MBD2) complex connects deacetylation, chromatin remodeling and DNA methylation; repressive function CHROMO and PHD domains bind methylated lysines on histone tails modulate activity of remodelers ATPase domain ROLE of CHD ATPase domains

9 INO80 chromatin assembly DNA repair interacts with phosphorylated H2A.X (gammaH2A.X) transcription SWR1 H2A exchange with H2A.Z Boundary to heterochromatin spreading transcriptionally poised promoters (together with H3.3)

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14 WINAC-WICH WSTF Williams syndrome transcription factor

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23 helix–turn–helix motif (HTH) tight chromatin binding

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25 stepwise assembly model for transcription initiation.


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