Presentation on theme: "The Cell Nucleus and the Control of Gene Expression"— Presentation transcript:
1 The Cell Nucleus and the Control of Gene Expression CHAPTER 12The Cell Nucleus and the Control of Gene Expression
2 IntroductionAll cells in a multicellular organism contain the same complement of genes.Cells express their genetic information selectively.Gene expression is controlled by regulatory machinery in the cell nucleus.
4 12.1 The Nucleus of a Eukaryotic Cell (1) The contents of the nucleus are enclosed by the nuclear envelope.A typical nondividing nucleus includes:Chromosomes as extended fibers of chromatin.Nucleoli for rRNA synthesis.Nucleoplasm as the fluid where solutes are dissolved.The nuclear matrix, which is the protein-containing fibrillar network.
5 The Nucleus of a Eukaryotic Cell (2) The Nuclear EnvelopeThe nuclear envelope is a structure that divides the nucleus from its cytoplasm.It consists of two membranes separated by a nuclear space.The two membranes are fuses at sites forming a nuclear pore.The inner surface of the nuclear envelope is lined by the nuclear lamina.
7 The Nucleus of a Eukaryotic Cell (3) The nuclear laminaSupport the nuclear envelope.It is composed of lamins.The integrity of the nuclear lamina is regulated by phosphorylation and dephosphorylation.
10 The Nucleus of a Eukaryotic Cell (4) The Structure of the Nuclear Pore Complex and its Role in Nucleocytoplasmic ExchangeProteins and RNA are transported in and out of the nucleus.Nuclear pores contain the nuclear pore complex (NPC) that appears to fill the pore like a stopper.NPC is composed of ~30 proteins called nucleoporins.
15 The Nucleus of a Eukaryotic Cell (5) Proteins synthesized in the cytoplasm are targeted for the nucleus by the nuclear localization signal (NLS).Proteins with an NLS stretch bind to an NLS receptor (importin).Conformation of the NPC changes as the protein passes through.RNAs move through the NPCs as RNPs and carry NES (nuclear export signals) to pass through.
16 Importing proteins from the cytoplasm into the nucleus
17 Importing proteins from the cytoplasm into the nucleus (continued)
18 The Nucleus of a Eukaryotic Cell (6) Chromosomes and ChromatinPackaging the GenomeChromosomes consist of chromatin fibers, composed of DNA and associated proteins.Each chromosome contains a single, continuous DNA molecule.
19 The Nucleus of a Eukaryotic Cell (7) Nucleosomes: The Lowest Level of Chromosome OrganizationThe protein component of chromosomes include histones, a group of highly conserved proteins.Histones have a high content of basic amino acids.
20 The Nucleus of a Eukaryotic Cell (8) DNA and histones are organized into repeating subunits called nucleosomes.Each nucleosome includes a core particle of supercoiled DNA and histone H1 serving as a linker.DNA is wrapped around the core complex.The histone core complex consists of two molecules each of H2A, H2B, H3, and H4 forming an octamer.
24 The Nucleus of a Eukaryotic Cell (9) Histone modification is one mechanism to alter the character of nucleosomes.DNA and histones are held together by noncovalent bonds.
25 The Nucleus of a Eukaryotic Cell (10) Higher Levels of Chromatin StructureA 30-nm filament is another level of chromatin packaging, maintained by histone H1.Chromatin filaments are organized into large supercoiled loops.The presence of loops in chromatin can be seen:In mitotic chromosomes form which histones have been extracted.In meiotic lampbrush chromosomes from amphibian oocytes.
29 The Nucleus of a Eukaryotic Cell (11) Heterochromatin and EuchromatinEuchromatin returns to a dispersed state after mitosis.Heterochromatin is condensed during interphase.Constitutive heterochromatin remains condensed all the time.Facultative heterochromatin is inactivated during certain phases of the organism’s life.
30 The Nucleus of a Eukaryotic Cell (12) Constitutive heterochromatin :Found mostly around centromeres and telomeres.Consists of highly repeated sequences and few genes.Facultative heterochromatin:Is found in one of the X chromosomes as a Barr body through X inactivation.X inactivation is a random process, making adult females genetic mosaics.
32 The Nucleus of a Eukaryotic Cell (13) The Histone Code and Formation of HeterochromatinThe histone code hypothesis states that the activity of a chromatin region depends on the degree of chemical modification of histone tails.Histone tail modifications influence chromatin in two ways:Serve as docking sites to recruit nonhistone proteins.Alter the way in which histones of neighboring nucleosomes interact with one another.
34 The Nucleus of a Eukaryotic Cell (14) Heterochromatin has many methylated H3 histones, which stabilize the compact nature of the chromatin.Small RNAs and specific enzymes play a role in histone methylation.
35 Example of proteins that bind selectively to modified histones
36 Correlation between transcriptional activity and histone acetylation
37 Model showing possible events during formation of heterochromatin
38 The Nucleus of a Eukaryotic Cell (15) The Structure of a Mitotic ChromosomeChromatin of a mitotic cell exists in its most highly condensed state.Staining mitotic chromosomes can provide useful information.A karyotype is a preparation of homologous pairs ordered according to size.The pattern on a karyotype may be used to screen chromosomal abnormalities.
41 The Nucleus of a Eukaryotic Cell (16) TelomeresThe end of each chromosome is called a telomere and is distinguished by a set of repeated sequences.New repeats are added by a telomerase, a reverse transcriptase that synthesizes DNA from a DNA template.
43 The Nucleus of a Eukaryotic Cell (17) Telomeres (continued)Telomeres are required for the complete replication of the chromosome because they protect the ends from being degraded.Telomerase activity is thought to have major effects on cell life.