2. Medical Enzymology By Amr S. Moustafa, M.D.; Ph.D. Assistant Prof. & Consultant, Medical Biochemistry Dept. College of Medicine, KSU amrsm@hotmail.com

3. Enzymes: Recommended Names  Substrate + -ase e.g., Sucrase, Lactase  Action: Lactate dehydrogenase  Others: Pepsin, Trypsin

4. Enzymes: Systematic Names  Lactate:NAD + Oxidoreductase  Complete description of chemical reaction  Six major classes of enzymes

5. Enzymes: 6 Major Classes

6. Aminotransferases

8. Hydrolases

9. Isomerases

10. Ligases

11. Substrate Concentration: Hyperbolic Vs Sigmoid Curve

12. Michaelis-Menten Equation  E + S ES E + P  v ° = K m : Michaelis constant = (k-1 + k2) / k1 k1 K-1 k2 V max [S] K m + [S]

13. Michaelis-Menten Kinetics

14. Zero and First Order Reactions

15. Lineweaver-Burk Plot = + 1 v°v° KmKm V max [S] 1 V max