1. The assessment of the effects of low- intensity laser radiation on cells of tumor strain of kidney cancer PA-1 Viktoria V. Skvortsova, Grigory E. Brill, Galina A. Afanasyeva, Alla B.Bucharskaya Saratov State Medical University, Russia

2. Purpose The purpose of the study was to investigate the effects of low-intensity red laser in vitro on cells of tumor strain of kidney cancer PA-1 and assess the tumor transplantability in rats after laser irradiation, macro- and microscopic

3. Materials and methods 12 white outbred male rats weighing 200 ± 20 g were used in the experiments. The tumor strain of kidney cancer PA-1 was obtained in Bank of tumor strains of Oncological Centre n.a. N.N. Blokhin.

4. Materials and methods Immediately after removal of tumors from donor rat, cancer tissue weighing 30 g was washed with physiological saline, added to 10 ml of Hanks solution and mechanically milled.

5. Materials and methods

7. Methods

8. Results Significant differences were marked on the 17th day of the experiment, when the average tumor volume was 10,2 ± 2,8 cm 3 in the experimental group, 2,26 ± 0,75 cm 3 - in the control group (p<0,02).

9. Dynamics of tumor growth

10. Results On the 19th day of experiment the average tumor volume was 13,9 ± 1,6 cm 3 in the experimental group, 4,03 ± 0,77 cm 3 - in control (p<0,001).

11. Results Finally, on the 21st day the size of transplanted tumors was 23,0 ± 3,8 cm 3 in the experimental group, 10,4 ± 2,2 cm 3 - in control (p<0.02).

12. Histologically, we did reveal some differences in samples of tumors between experimental and control groups.

13. We observed cells with degenerative changes, with increased eosinophilia of the cytoplasm, hyperchroma- tic and smaller nuclei, that occupy from 40 to 70% of the area. Lumen of blood vessels occur from single to 10 in sight.

14. Conclusion

15. Some questions without answers… How does biological activity of kidney cancer cells modify under the influence of laser irradiation? Does laser change genetic apparatus of cancer cells? How long and unambiguous is effect of exposure at these characteristics of the laser?

17. Thanks for your attention!!!