1. ENVIRONMENTAL CONTROL FOR A CHIP SCALE LABORATORY Team: Robert Bouda - Leader Ashok Rajan – Communication Liaison Sean Belieu – Webmaster Sahan Fernando Tianyu Wang Zhaokui Wang Faculty Advisor & Client: Dr. Liang Dong

2. System Description  Problem Need Statement: A greenhouse is costly, space intensive, and we are looking to solve it.

3. Functional Requirements Solenoid Control System 1. A system using Verilog or VHDL capable of receiving and decoding a serial digital signal Sensor Array 1. A control element that sends data in a serial digital format to a different system designed to regulate environmental conditions Channel and Chamber fabrication 1. A flow channels capable of delivering liquid to individual chambers at volumes of 1µm3. 2. 7 air flow channels designed to depress and restrict flow through liquid channels using air pressure. Risks Channel thickness

4. Solenoid Valves

5. Solenoid Valves The connections with the chip are done using thin tubing and syringes:

6. Solenoid Valves Clippard(12-ET-2M-6) Extremely basic solenoids 25-pin interface Multisim – Verilog Existing technology: Fluidigm Genetic Analysis

7. Work plan – Subgroup 1 This semester: Design the control logic of solenoid valves to: a) Control the liquid flow into the growth chambers b) Select a specific growth chamber out of 128 growth chambers that are fabricated on an interface. Next semester: Intergrate sensor unit and fabricated channels with Solenoid control

8. Sensor Unit  The sensor detects temperature/ humidity and outputs the data via serial DATA to a micro- controller.  This data will be outputted via the micro-controller in binary form to the solenoid control system.

9. Work plan – Subgroup 2  This semester: A sensor unit that will successfully read environmental Temperature/Humidity and output data to the control system  Current: Actual circuit design Coding the Micro-controller  Next Semester: Intergrate sensor unit with Solenoid control

10. There are 128 micro-greenhouses totally. And there are 128 channel connect to each of them. 7 bits are used to make them work individually.(2^7=128) (As shown above) Microfluidics Channels

11. Fabrication process Firstly, we fabricate our molds on glass slide by using IBA. Then Once the slides are ready, we used as molds for PDMS, when the control and flow layers is prepared separately we bond them together crossing to each other.

12. Two layers device The designed chip has two layers: flow level(Top), where the cells will ultimately grow. control layer(Under), used to close and open certain valves by putting under pressure the thin membrane between the control and the flow layers.

13. Work plan – Subgroup 3  This semester: Learning how to fabricate. Learn fabrication with the IBA( isobornyl acrylate) process Learn PDMS(Polydimethylsiloxane) fabrication process Make a two layers devices and perform tests on it to ensure quality of devices.  Next Semester: Ensure interface that was created serves as a good mediator between the other two sub projects.

14. Projected Costs MaterialProduct (Not Final)Cost ( Estimation) Solenoid Valves (Qty-12) Clippard 12 ET – 2M – 6 $340.00 Electronic Manifold (Qty-1) Clippard EMC – 12 – 06 – 20 $260.00 Temeprature & Humidity sensor (Qty-1) Sensiron SHT11 $40.00 Tubing Adaptors (Qty-12)- $30.00 Tubing Cole Palmer EW-06418-02 Donated by Client IBA Fabrication Glass-Donated by Client MCU for Sensor and Solenoids-Currently Available Lab Equipment Total$670

15. Final Deliverables  The expected end product will consist of three sub-systems that will be combined to form the complete chip-scale greenhouse system End Product Temperature and Humidity Sensing Unit Solenoid Control System Liquid flow channels

16. Questions?