1. Effect to temperature on urease
Lab report
Effect to temperature on urease
Member : Pim Pat Gail First Max
mandarin 10-2

2. In this experiment help us to investigate the enzyme urease by using the temperature to observed the substrate concentrations affect enzyme activity.

3. Procedure 1
-2 drops of the critic acid with indicator change their color to light pink.
-2 drops of baking soda with the indicator made the color change from purple to blue.
-2 drops of dH20 made the indicator change their color to become lighter.

4. 1. Why is it important to have the three reference tubes. 2
1. Why is it important to have the three reference tubes? 2.Can you predict what color of the solution will be if the urease enzyme convert urea to ammonia?

5. Test tube Color change 2 drops of critic acid with an indicator
2 drops of baking soda with an indicator
Color change
Purple to light Pink
Purple to Blue
Color get lighter

6. Procedure2
>>Urea + Indicator, the color stays the same and not change.
>>Enzyme + Indicator, the color didn't change
>>Urea + Enzyme + Indicator, change from purple to blue.

7. 1. What are the purpose of procedure 2. 2
1.What are the purpose of procedure 2? 2.Why do we need to add an enzyme into a test tube without urea even though we can predict that o enzyme activity will occur?

8. Urea + Enzyme + Indicator
Test Tube
Urea + Indicator
Enzyme + Indicator
Urea + Enzyme + Indicator
Color change
No change
Purple to Blue

9. Procedure 3
>>2 ml of Soya suspension before adding Urea and Indicator
Test tube in ice bath: Remain normal
Test tube at room temperature(25°C): Remain normal
Test tube in water bath (70°C): enzyme denatured
Test tube at 50°C temperature (45°C): Remain normal
Test tube in boiling water: Enzyme denatured

10. Enzyme denatured (Remain the same)
Test Tube
Ice bath
Room temperature
Water bath
50 temperature
Boiling water
Temperature°C 25 70 45
100
Color change
Remain normal
enzyme denatured
Enzyme denatured (Remain the same)

11. 1.How does the temperature affect the urease activity? How do you know ? 2.From your result, what temperature would you use and avoid if you have to conduct an experiment using urease enzyme and why? 3.Descrive the appearance of the denatured urease enzyme in the test tube?

12. Result after adding Urea and Indicator
When return into the test tube:
Test tube in ice bath: Stay the same (purple)
Test tube at room temperature(25°C): Does not denatured and change from purple to green
Test tube in water bath (70°C): Not all denatured (We can see that active site can process).
Test tube at 50°C temperature (45°C): Enzyme not denatured, change from purple to green
Test tube in boiling water: Enzyme denatured, stay the same

13. Observed the color change after 5 minutes
Test tube in ice bath: Turned from purple to light blue. We figured out that enzyme working when the temperature get warmer.
Test tube at room temperature(25°C): Does not denatured and change from green to dark green
Test tube in water bath (70°C ): Not all denatured, turned to green (We can see that active site can process).
Test tube at 50°C temperature (45°C): Enzyme not denatured, change from green to dark green
Test tube in boiling water: Enzyme denatured, stay purple.

14. Conclusion
-We have learn how much temperature will kill enzyme by putting 4 test tube in 4 different temperatures.
-It have some like when put enzyme at room temperature the bigger was open stove first then it make the temperature go very high we need to be careful before doing experiment
-The prediction that we make before was enzyme will be survive in every temperature but it doesn't when we make experiment it can't be survive at 100 degree Celsius and at 0 degree Celsius it was died but not all of them.