1. Cherry leaf roll disease Sample Collection & Analysis

2. Types of samples to collect Budsticks Collect from the end of January until bud break About 8” at the base of the 1-year-old wood One stick from each scaffold or symptomatic limb

3. Types of samples to collect Flower & leaf buds Bud swell to flower bud popcorn stage Six buds in total Collect from all major limbs and branches

4. Types of samples to collect Leaves Collect 2 weeks after full bloom until the end of June Collect the first leaves that emerge in spring (oldest) Collect six leaves in total from each tree Collect from all major scaffold limbs including any symptomatic limbs

5. Collect samples from all major limbs Virus distribution is erratic, particularly with recent infections Insure that part of each sample is collected from symptomatic shoots or from each major limb

6. Ship samples in ziplock bags Seal samples in ziplock bags (don’t add extra moisture) Label bag (tree, row, grower, GPS) Include contact information Keep out of direct sun Keep cool (40F), but don’t freeze Overnight delivery to WSU ELISA Lab (early to mid-week)

7. Shipping Information ELISA Laboratory Washington State University-IAREC 24106 N. Bunn Rd. Prosser, WA 99350 Contact: Tina Vasile ELISA Lab Manager 509-786-9382 tina_vasile@wsu.edu Ken Eastwell Project Manager 509-786-9385 keastwell@wsu.edu Ship overnight delivery via courier to: Switchboard: 509-786-9382 Facsimile: 509-786-9370