1. Cellular Physiology of Secretion and Absorption Johnson Chap. 34-35 Jack L. Leonard 2004

2. Johnson Chap. 34-35 FluidVolume(ml/day)pH Saliva1000-12006.0-7.0 Stomach (gastric secretion) 2000-30001.0-3.5 Pancreas (exocrine secretion) 1200-20008.0-8.3 Bile secretion 600-7007.8 Brunner’s gland secretion 508.0-8.9 Succus entericus (intestinal juice) (intestinal juice)2000-30007.8-8.0 Large intestine 607.5-8.0 Daily Secretion of Digestive Fluids

3. Johnson Chap. 34-35 Na Na P K K K Na Outside Inside Apical membrane Basolateral membrane Basic Secretory Mechanisms (Gut lumen) (interstitial space) Redrawn from Acta Physiologica Scand. 42:298, 1958

4. Johnson Chap. 34-35 Apical Membrane Johnson Chap. 34-35 (Gut lumen) (interstitial space) POutsideInside Apical Basolateral Na + K+K+K+K+ Cl - Channels Na + glucose Na + coupled organic Cl - HCO 3 - Antiporter Na + K+K+K+K+ 2Cl - Symporter Apical Surface

5. Johnson Chap. 34-35 Basolateral Membrane Johnson Chap. 34-35 Na + H+H+H+H+Antiporter K+K+K+K+ 2Cl - Symporter K+K+K+K+ ActiveTransporter Na + ATP (Gut lumen) OutsideApical Basolateral P (interstitial space) Inside Basolateral Surface

6. Johnson Chap. 34-35 5101520 10 20 30 40 Na + (mM) (Intracellular) (Intracellular) Transport Rate Na Pump Transport Rate

7. Johnson Chap. 34-35 Gut lumen interstitial space Cl - Na + K+K+K+K+ Cl - Na + K+K+K+K+ K+K+K+K+ Salt Secretion interstitial space Salt Absorption Na + K+K+K+K+ K+K+K+K+ Cl - Na +

8. Johnson Chap. 34-35 12 3 40 20 40 60 80 100 Ion concentration (mM) Flow (ml/min) Na HCO 3 Cl K 20 40 60 80 100 120 140 0 Ion concentration (mM) NaCl HCO 3 K Saliva Plasma Salivary Duct Cell

9. Secretion of Na +, K +, and Cl - Salivary Acinar Cell Johnson Chap. 34-35 Na Na Na + K+K+K+K+ Cl - K+K+K+K+ ATP Na + K+K+K+K+ Cl - K+K+K+K+ Lumen Serosal surface stimuli

10. Johnson Chap. 34-35 Na + K + Cl - CO 2 K+K+K+K+ ATP Na + Cl - K+K+K+K+ Lumen Serosal surface Na + H+H+H+H+ CO 2 H 2 CO 3 HCO 3 - Na + K + Cl - HCO 3 - Salivary Duct Cell

11. Johnson Chap. 34-35 12 3 40 20 40 60 80 100 Ion concentration (mM) Flow (ml/min) Na HCO 3 Cl K 20 40 60 80 100 120 140 0 Ion concentration (mM) NaCl HCO 3 K Saliva Plasma Salivary Duct Cell

12. Johnson Chap. 34-35 100 300 500 0 Secretion rate (ml/hr) 0 160 120 80 40 Ion Concentration (mM) 7.8 7.4 8.2 pH 300 260 340 Osmolality Osm pH Na + HCO 3 - Cl - K+K+K+K+ Pancreatic Secretion

13. Johnson Chap. 34-35 Intestinal Absorbing Cell Na + K+K+K+K+ Cl - Cl Cl K+K+K+K+ATP Na + K+K+K+K+ Lumen Serosal surface glucose Na + Cl - HCO 3 - K+K+K+K+ ATP Na +

14. Intestinal Secretory Cell Johnson Chap. 34-35 Na Na Na + K+K+K+K+ Cl - K+K+K+K+ATP Na + Cl - K+K+K+K+ Lumen Serosal surface stimuli K+K+K+K+ ATP Na +