1. Test 2 Review Chem 3550 10/13/06

2. Chapter 5 - Enzyme Kinetics Meaning of K M Effect of inhibitors on reaction parameters(K m, V max ) Competitive, non-competitive and uncompetitive inhibition and kinetics

3. Chapter 10 - DNA & RNA Structures Know molecular structure of bases Be familiar with nomenclature Be able to construct a DNA/RNA polymer Know factors that stabilize double helix Know different types of DNA (A, B, Z) Understand hyperchromic effect Know different types of RNA Understand exo- and endonucleases Understand packaging of DNA in the chromosome

4. Chapter 11 - DNA Replication & Transcription Know mechanism of DNA replication and transcription and enzymes involved Understand cause of mutations and know repair mechanisms Know RNA synthesis mechanism Know post-transcriptional modifications for tRNA and mRNA processing Understand DNA sequencing

5. Chapter 12 - Protein Synthesis Know components of ribosomal machinery Know how aminoacyl-tRNA forms Know steps of protein synthesis Know energy requirements of process Know how to stop the process Know how DNA-binding proteins function Understand the common motifs of regulatory proteins