Evidence for dynamically organized modularity in the yeast protein- protein interaction network Han, et al. 2004.
Published byModified over 3 years ago
Presentation on theme: "Evidence for dynamically organized modularity in the yeast protein- protein interaction network Han, et al. 2004."— Presentation transcript:
Evidence for dynamically organized modularity in the yeast protein- protein interaction network Han, et al. 2004
Preprocessing of PPI data Data sources: Yeast two-hybrid data (5,249) Affinity purifications followed by MS (6,630) Computational predications (7,446) MIPS protein complexes (9,597) FYI (Filtered Yeast Interactome): 2,493 interactions, each observed in at least two different data sources 1,397 proteins A large connected component of 778 proteins
Network of the connected component of 778 proteins
ribosomal protein L23a.e TFIID and SAGA subunit 60S large subunit ribosomal protein L5.e 60S large subunit ribosomal protein L27a.e 60S large subunit ribosomal protein L8.e 60S large subunit ribosomal protein L3.e 60S large subunit ribosomal protein
Expression correlation Hubs of PPI network Nodes with degree k greater than 5 mRNA expression data set of 315 conditions Five categories of conditions Average PPCs between the hub and each of its respective partners Pearson correlation coefficient
Red curve: average PCCs of hubs Cyan curve: average PCCs of non-hubs Black curve: average PCCS of hubs in randomized networks
Party and date hubs The bimodal distribution suggests that hubs can be split into two distinct populations: One with relatively high average PCCs (108 party hubs) The other with relatively low average PCCs (91 date hubs)
Date hubsParty hubsDate %Party % Total91108100% Regulator451349%12% Adaptor15616%6% Mediator10011%0% Complex168718%81% Other323%2% unclear202%0% Categories according to YPD annotations
Partners of date hubs are significantly more diverse in spatial distribution than partners of party hubs, according to proteome-wide cellular localization data set.
Gradual removal of nodes The characteristic path length, defined as the average distance (shortest path length) between node pairs, reflects the overall network connectivity. Green line: random removal of nodes; Brown line: attack on all hubs; Blue line: attack on party hubs; Red line: attack on date hubs;
Removal of date hubsRemoval of party hubs The largest connected component of the FYI network