1. Metagenomics Rob Edwards MCS

2. The Soudan Mine, Minnesota Red Stuff Oxidized Black Stuff Reduced

3. Red and Black Samples Are Different Cloned and 454 sequenced 16S are indistinguishable Black stuff Red Cloned Red

4. There are different amounts of substrates in each environment Black Stuff Red Stuff

5. There are different amounts of metabolism in each environment

6. Nitrification differentiates the samples Edwards (2006)

7. The SEED metagenomics server

8. Metabolic potential in environments

9. Phylogenomics

10. Comparing Metagenomes to Genomes (or other metagenomes!)

11. Lots of sequences all pyrosequencing

12. Metabolic potential in environments

13. Sulfur CDA 60.2% CDA 21.7% Respiration Capsule Motility Membrane transport Stress Signaling Phosphorus RNA Mine Saltern Marine Microbialites Coral Fish Animals Freshwater From sequences to environments Dinsdale et al. unpublished