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Use of dialysis to separate small and large molecules.

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Presentation on theme: "Use of dialysis to separate small and large molecules."— Presentation transcript:

1 Use of dialysis to separate small and large molecules

2 Gel filtration chromatography

3 Molecular mass determination by gel filtration chromatography

4 Some commonly used gel filtration materials

5 Ion-exchange chromatography with stepwise elution

6 Device for generating a linear concentration gradient

7 Molecular formulas of cellulose-based ion exchangers

8 Some biochemically useful ion exchangers

9 Affinity chromatography

10 Covalent linking of ligand to agarose

11 Derivatization of epoxy-activated agarose

12 Purification of staphylococcal nuclease by affinity chromatography on bisphosphothymidine-linked agarose

13 Experimental arrangement for paper chromatography

14 Two-dimensional paper chromatography

15 Paper electrophoresis

16 Apparatus for slab gel electrophoresis

17 Diagram of a disc electrophoresis apparatus

18 Polymerization of acrylamide and N,N-methylenebisacrylamide to form a cross-linked polyacryl-amide gel

19 SDS-PAGE

20 Logarithmic relationship between the molecular mass of a protein and its relative electrophoretic mobility in SDS-PAGE

21 General formula of the ampholytes used in isoelectric focusing

22 Isoelectric points of several common proteins

23 Purification of rat liver glucokinase

24 END

25 An enzyme-linked immunosorbent assay (ELISA)

26 Solubilities of several proteins in ammonium sulfate solutions

27 Solubility of caboxy-hemoglobin at is isoelectric point as a function of ionic strength and ion type

28 Solubility of  -lactoglobin as a function of pH at several NaCl concentrations

29 Protein crystals

30 Agarose gel electrophoretogram of a double helical DNA

31 Pulsed-field gel electrophoresis (PFGE) of a set of different sized bacteriophage DNAs

32 Detection of proteins by immunoblotting

33 Two-dimensional (2D) gel electrophoresis

34 Sedimentation coefficients in Svedbergs (S) for some biological materials

35 A selection of preparative ultracentrifuge rotors

36 Zonal ultracentrifugation

37 Equilibrium density distribution of a CsCl solution in an ultracentrifuge spinning at 39,460 rpm

38 Isopycnic ultracentrifugation

39 Physical constants of some proteins


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