1. BIO132 Lab 2 Exercise 29A/29 Blood
Human Blood Smear
40X objective (400X)
Leukocyte
Erythrocyte
Platelet

2. Neutrophils
40X objective (400X)

3. Eosinophils
40X objective (400X)

4. Basophils
40X objective (400X)

5. Lymphocytes
40X objective (400X)

6. Monocytes
40X objective (400X)

7. Human Blood Smear
40X objective (400X)
Leukocyte
Erythrocyte
Platelet

8. • Least dense component
Formed
elements
Plasma
• 55% of whole blood
• Least dense component
Buffy coat
• Leukocytes and platelets
• <1% of whole blood
Erythrocytes
Withdraw blood
and place in tube. 1
Centrifuge the
blood sample. 2
• 45% of whole blood
(hematocrit)
• Most dense component
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9. X 100 Hematocrit = % blood occupied by cells Height of cells in mm
Plasma
Buffy Coat
(WBCs + Platelets)
Erythrocytes
Air
Clay
Height of cells in mm
Height of total in mm
X 100
Expressed as %
Typical = 45%
Total
Cells

10. Antigens present on Erythrocytes:
Purified antibodies used to detect antigens:
Antibody + Antigen = agglutination reaction (clumping)

12. Lymphatic Leukemia
Normal Blood
40X objective (400X)

13. Normal Blood
Sickle Cell Anemia
40X objective (400X)