1. Lecture 34 11/30/05

2. Distribution coefficient

3. What is the distribution constant for a Base (B) with a K = 3.0 and a pK a = 9. (phase 1 = aqueous, phase 2 =organic) For an amine with the previous characteristics, what is the formal concentration remaining in the aqueous phase if 50 mL of 0.01M aqueous amine is extracted with 100 mL solvent at pH 10? At pH 8?

4. What is the distribution constant for an Acid (HA) with a K = 3.0 and a pK a = 3. (phase 1 = aqueous, phase 2 =organic)

5. Chromatography Packed vs. open tubular

6. Types of Chromatography Normal-phase vs. reverse phase

7. Types of Chromatography

8. Copper Speciation

9. Chelex-100 Ion Exchange Resin

11. Types of Chromatography