1. Bimonthly Meeting on Oct. 10, 2008 Metabolic Concentrations and Ratios of Brain Tissue Amarjeet Bhullar

2. We have compared metabolite ratios, calculated using different methods: Directly Generated by Functool [F1] Using Metabolites Concentration [F2] Gaussian Function Overlapped on metabolite peak with Baseline correction Gaussian Function Overlapped on metabolite peak with Baseline+Noise correction

3. Baseline Correction Baseline corrects separately each individual spectral point in spectrum By calculating and subtracting a Spline through each points for smoothing. If smoothing width is non zero, takes its average value, which is very small =zero In most of our spectrum baseline exists at zero

4. Baseline+Noise Correction Metaboliteppm rangeImage range Choline3.16-3.3267-77 Creatine2.94-3.1080-90 NAA1.94-2.10147-158 Lipid and lactate1.50-0.90187-221 Noise=Average value of after removing of four peaks

5. Chemical Information on Voxel # 16, 19, 20

6. Chemical Information on Voxel # 24, 25, 26

7. Chemical Information on Voxel # 27, 30, 31

8. Metabolite Ratios Cho/Cre

9. Conclusion : There is no significant difference between our calculation and Functool. Suggestions are welcome