1. Gene annotation: Nitrogen transporter families and N metabolism Annick Brun, Eva Lucic, Marc Buée, Barbara Montanini, Damien Blaudez, Francis Martin et Michel Chalot. INRA-Nancy, 5 avril 2006 Unité Mixte de Recherche 1136 INRA/Université Henri Poincaré Interactions/Arbres Micro-Organismes IFR "Génomique, Ecophysiologie et Ecologie fonctionnelle" BP 239 - 54506 Vandoeuvre-lès-Nancy Cedex, France

2. Marmeisse, R., et al. (2005) New Phytologist 163 (3), 481-498.

4. Laccaria bicolor proteases Approximately 130 proteases in L. bicolor Caspase: involved in apoptosis Fungalysin: membrane attachments, remodeling of cytoskeletal, apoptosis Calpain: probably contributes to destruction of host tissues in infection (plant and animal infections)

5. Ascomycota cluster (Trichoderma, Aspergillus oryzae, A. nidulans) Basidiomycota cluster 2 (Coprinus, Laccaria, Phanerochaete, Ustilago) Potentially involved in the degradation of the OM Basidiomycota (Hymenomycetes) cluster 1 (Coprinus, Laccaria, Phanerochaete, Coccidioides) Basidiomycota cluster 3 (Coprinus, Laccaria, Phanerochaete, Ustilago)

6. OPT

8. Phanerochaete chrysosporium Coprinus cinereus Laccaria bicolor Cryptococcus neoformans Ustilago maydis Tetra / pentapeptide and glutathione transport

9. Marmeisse, R., et al. (2005) New Phytologist 163 (3), 481-498.

11. L-type Amino Acid Transporter (LAT) Yeast Amino Acid Transporter (YAT) Amino Acid/Choline Transporter (ACT) Amino Acid/Auxin Permease (AAAP)

13. 10 gene models

15. AMT expression by RT-PCR Amino acids content Glutamine content AMT3.1 AMT1 AMT2.1 AMT13.2 AMT13.4 T0 -N48 +N24 Carpo A B

16. x2 - x4

20. Amino Acid Metabolism 494 Alanine and aspartate metabolism 25 Arginine and proline metabolism 34 Cysteine metabolism 9 Glutamate metabolism 32 Glycine, serine and threonine metabolism 46 Histidine metabolism 30 Lysine biosynthesis 22 Lysine degradation 46 Methionine metabolism 17 Phenylalanine metabolism 24 Phenylalanine, tyrosine and tryptophan biosynthesis 18 Tryptophan metabolism92 Tyrosine metabolism36 Urea cycle and metabolism of amino groups20 Valine, leucine and isoleucine biosynthesis12 Valine, leucine and isoleucine degradation31 KEGG Metabolic Pathways Energy Metabolism 130 ATP synthesis 17 Carbon fixation 19 Methane metabolism 3 Nitrogen metabolism 32 Oxidative phosphorylation 37 Photosynthesis 1 Reductive carboxylate cycle (CO2 fixation) 14 Sulfur m etabolism 7

21. GDH à NADPH 1,1,1 Glutamate synthase NADPH 1,1,1 GS (1,1,1) Asparaginase 3,0,1 GDH à NAD 2,1,1 Asparagine synthase 1,1,1 Aspartate Amino Transférase (2,3,3) Alanine Amino Transférase (1,1,1) Laccaria laccata Phanerochaete chrysosporium Cryptococcus neoformans 2-nitropropane dioxygenase (1,1,1) Cystathionine gamma-lyase 4,1,5 Phenylalanine ammonia-lyase 2,1,2 D-amino-acid dehydrogenase1,2,1 Aminomethyltransferase 2,1,1 Carbamoyl-phosphate synthase 1,0,1 Carbonate dehydratase 9,3,0 Nitrate reductase1,1,1 Nitrite reductase 1,1,1

22. Cycle de lurée 1 1 2 2 Arginase (1,1,1) Argininosuccinate lyase (2,1,1) Argininosuccinate synthase (2,1,1) Ornithine carbamoyle transferase (2,1,1) Carbamoyl- phosphate synthase (1,1,1) Aminoacylase (1,1) Acetylornithine aminotransferase (2,2,2) Glutamate N- acetyltranferase (1,1,1) Amino-acid N- acetyltransferase (2,2,2) Glutamate 5- kinase (1,1,1) Ornithine decarboxyase (1,1,1) Urease (1,1,1) Guanidinoacetate N- methyltransferase (1,1,1) Glutamate-5- semialdehyde dehydrogenase (1,1,1) Ornithine-oxo-acid aminotransferase (1,1) Pyrroline-5-carboxylate reductase (1,1) Protein ARG5,6 (1,1) Spermidine Synthase (1,1,1) Chimeric Spermidine Synthase/ Saccharopine Dehydrogenase (1,2,1) Laccaria laccata Phanerochaete chrysosporium Cryptococcus neoformans

24. Read, D. J. & Perez-Moreno, J.(2003) New Phytologist 157 (3), 475-492.