1. Team Pegasus MSF SatElysium Critical Design Review Jordan Burns, Brenden Hogan, Miranda Link, Cody Spiker, Chris Dehoyos, Hemal Semwal October 4 th, 2011

2. Mission Overview Mission Statement: “To test the effects of high altitude flight upon a closed contatiner containing bacterium and recording their response to discover if harsh high altitude effects bacteria l reproduction and survival.” Objective: Test and record the effects of a near space environment on several cultures of Streptococcus mutans. Purpose: Determine how bacteria cultures respond to the lack of pressure, extreme temperatures, and radiation present in near-space. Why: Test the validity the of the recorded results of the 1967 flight of Surveyor III. According to their results, we think that we will find that the bacteria not only survive, but actually thrive in the harsh environment of space.

3. Requirements Flow-Down Level 0 LevelRequirementDescriptionOrigin 0 AConstruct a BalloonSat that shall survive an ascent to 30 km above the surface of Earth and the following decent while maintaining complete functionality. Mission Statement BThe weight of SatElysium shall not exceed 850 grams, nor a budget of $370. CSatElysium shall safely transport 6 samples of streptococcus mutans during its flight, studying the effects of temperature and radiation in the stratosphere on the bacteria. DThe streptococcus mutans samples shall be recovered and analyzed post-flight. ESatElysium shall carry a camera payload to document footage of the flight of the exterior of the satellite.

4. LevelRequirementDescriptionOrigin 1 A.1 Build a cubical structure that measures 21 cm in height, width, and length out of foam core, hot glue, and aluminum tape. The structure shall contain a rod that shall attach the satellite to the flight string for the duration of the flight. Level 0 B.1 The weights of all components shall be monitored during build and the entire satellite shall be weighed prior to launch. B.2 Miranda Link shall maintain an updated budget and keep all team members informed of its status. C.1 Three separate environments shall be created on board the satellite: one that is insulated and heated, one that has no heater, and one that has no heater and is expose to radiation. C.2 Samples shall be secured to the structure of the satellite with velcro. C.3 Samples shall contain the bacterium streptococcus mutan. C.4 Temperature and radiation data shall be collected at regular intervals by a system on board D.1 Obtain access to a microscope that is of sufficient power to analyze our microbes D.2 Analyze microbes before and after flight as well as conducting a variety of ground control test. E.1 Appropriate space for the system in design phases as well as a way for the camera to see out of the satellite. E.2 Install on board the satellite and program with proper instructions. Requirements Flow-Down Level 1

5. Design 21 cm cube 2 levels Bacteria - 6 different samples, 3 locations with varying conditions Radiation Temperature Heated and isolated Ground control (4) samples Experiment structure Petri-dish orientation – tilted for space Motorized door to expose one sample to the outside environment Petri-dish support structure Analysis of Bacteria (before and after flight) Colony Count (avg.) Color Spores

6. Design Microcontroller Pressure Temperature Light Hobo Sensors Heater (2) For technical components and (1) bacteria sample

7. Functional Block Diagrams

8. Design Parts: Solder Foam Core HOBO Arduino Pro Digital Camera Heating System Divided Petri Dishes 9 volt Batteries Aluminum Tape Humidity & Temp. Sensor Styrofoam Live Strand of Streptococcus Mutans AGAR Motor Light Sensor- NOT ORDERED Pressure Sensor – NOT ORDERED

9. Schedule 9/12/11: Divide tasks and submit individual sections by 9/13/11 9/14/11: Team meeting/Take ITLL Tour to get access 9/15/11: Finalize Proposal 9/16/11: Submit Proposal 9/19/11: Team meeting for Design Presentation 9/20/11: Conceptual Design Review Presentation 9/22/11: Team meeting to decide parts order forms. 9/27/11: Order satellite hardware 9/28/11-10/7/11: Build and test prototypes. Grow first set of bacteria for ground control. 10/8/11-10/13/11: Complete testing and design modifications. 10/16/11-10/23/11: Construct BalloonSat. 10/23/11: Satellite completion 10/25/11: Pre-Launch Inspection 10/27/11: In class mission simulation 11/01/11: Launch readiness review 11/04/11: Final BalloonSat Weigh in and turn in 11/05/11: Launch and Recovery 11/06/11: Meet to review data 11/14/11: Review final report 11/21/11: Complete final report 11/29/11: Final Team Presentations and Report 12/03/11: Design Expo

10. Test Plan Our testing will occur in two separate phases. The first of these phases will take place during the weekend of October 7-9. Structural integrity tests Whip test Drop test Roll test The second set of tests will occur after we have all of our materials gathered and ready to perform the necessary experiments. The Incubation and Vacuum Chamber The Cooler test will be the last thing need the entire completed BalloonSat ready to run a full-time data collection trial with all components of the working spacecraft in order. The software/hardware testing will occur repeatedly throughout our entire building process as things will need to be adjusted every so often.

11. Testing Phase I: Structural Testing Drop/ Roll Test Roll down flight of stairs Drop from a height of 15 meters Whip Test Spinning the Balloonsat by the tether running through it. Making sure that the balloonsat can withstand the forces acting upon it during the flight environment. Different starting orientations to ensure proper testing

12. Testing cont’d Phase II: Scientific Materials Testing Incubation Test Temperature, light, and pressure controlled environment to have a sample. Grown bacteria cultures throughout the semester to analyze a “natural” growth pattern. Vacuum Chamber Test Hand in Hand with the incubation test, merely used as a ground test to show the ground effects of the bacteria with a “zero pressure” environment Software / Hardware Testing Recursive tests involving the wiring and technical setup of the camera and temperature sensor arrangement to allow for different capturing intervals of the camera and data collections by the individual sensors that we have. Cooler Test Final test of our satellite. Will ensure that all the technical components will last for the duration of an entire flight sequence at temperatures exceeding -70­°F.

13. Expected Results We expect to observe bacteria response, bacterium count, health, reproduction, death and other anomalies Compare resulting bacterium tests of both the baseline as well as the space bacteria. use a microscope Specifically look at spore count We expect to find that the bacteria will be resilient enough to survive in the harsh environment. Since our satellite will have three separate environments for testing, there is a real chance of seeing a change between each environment.

14. Budget ITEMSUPPLIERPRICE Weight (g) QuantityTotal US flagGateway ClassProvided to us<1 g1$0 SolderGateway ClassProvided to us<1 g1$0 Foam CoreGateway ClassProvided to us<100 g3$0 Hobo Data LoggerGateway ClassProvided to us30 g1$0 Digital CameraGateway ClassProvided to us130 g1$0 Heating systemGateway ClassProvided to us100 g1$0 Aluminum TapeGateway ClassProvided to us<3 g 1$0 9 volt batteryGateway ClassProvided to us150 g7$0 Arduino UnoSparkFun Electronics$29.95<5 g 1$29.95 Arduino Ambient Light Density Cds Photoresistor Sensor Biozoner$4.50 + $15 S&H40 g 1$19.50 Pressure Sensor SparkFun Electronics$15.96 +$2 S&H1 g 1$17.96 Humidity & Temp. Sensor SparkFun Electronics$9.95 + $2 S&H<20 g 1$11.95 StyrofoamMcGuckin’s Hardware$3.24 <30g 1$3.24 Live Strand of Streptococcus mutans Ward Science$9.95 + $7.80S&H1.5 g 1$17.75 AGARScientific Strategies $40.80 + $22.17S&H 1$62.97 Stepper Motor Anaheim Automation $14.3822.7 g 3$43.14 Petri Dish (20) Carolina Biological Supply Company $8.50 +$17.95 S&H<20 g 1$26.45 Dry IceSafeway<$15.00 1$15.00 Total Weight = 699.6 g Total Price = $249.92 incl. S&H

15. Team Organization Jordan Burns Project Manager -responsible for all management and scheduling -thermal engineer 9118 Andrews Hall, Boulder, CO 80130 (719) 337-5357 Jordan.Burns@Colorado.edu Brenden Hogan -Lead Electrical Engineer -responsible for circuits and mechanisms 9023 Crosman Hall, Boulder, CO 80310 (303) 483-1161 brenden.hogan@colorado.edu Miranda Nicole Link -Design and Budget Management -records of all team expenses, within budget and out of pocket. 590 Merlin St., Lafayette, CO 80026 (970) 372-8873 Miranda.link@Colorado.edu Hemal Sewal -Lead Structural Engineer -responsible for overseeing the construction of the satellite 9038 AdenHall, Boulder, CO, 80309 (719)-339-7570 Hemal.Sewal@Colorado.edu Cody Spiker -Science Manager -responsible for all control tests on the bacteria and the growth of bacteria cultures pre-launch 9023 Crosman Hall, Boulder, CO 80310 (970) 589-5689 Cody.Spiker@colorado.edu Christopher Dehoyos -Video Director and Testing Manager -responsible for designing all tests on the BalloonSat -oversee extra credit video 9130 Darley North Hall, Boulder, CO 80310 (210) 573-8448 Christopher.dehoyos@Colorado.ed u

16. Worries 1.That our petri-dishes may not properly fit in SatElysium. 2.That our insulation system will not properly separate the separate sections into their individual environments that need to be attained. 3.Attaining the proper structural integrity that will allow us to successfully have two separate levels in our SatElysium.