1. Ethical issues and the Three Rs Penny Hawkins Research Animals Department, RSPCA, UK phawkins@rspca.org.uk p Helping animals through welfare science

2. Developing processes of ethical review Promoting fuller implementation of the 3Rs: Replacement, Reduction & Refinement

3. Ethical framework in ASPA ASPA establishes an ethical framework  assess and weigh harms and benefits  make decisions on justification and necessity Different perspectives on harms and benefits  definitions of harms ... and potential causes of harm

4. The entire lifetime experience Breeding protocols Transport Laboratory housing Husbandry, care and environment Handling and restraint Identification Phenotyping Scientific procedures Adverse after- effects Euthanasia One person’s “best practice” can be another person’s baseline standard

5. Benefits of medical research Diseases cause suffering in humans  acute or chronic pain  distress due to mental disorders  physical degeneration  anxiety, distress, depression

6. Justification Alleviation of human suffering Opinion polls show 70 % support animal research for medical progress There are caveats associated with both of these There is still a serious ethical dilemma associated with creating animal models of disease

7. Reasons for reducing suffering Animal welfare benefits Better science Economic benefits Reassuring the beneficiaries of the research (patients and funders) Legal requirements

8. Approaches to refinement Ensuring that adverse effects are fully recognised and taken into account Thinking about animals’ basic needs Defining an effective welfare assessment scheme; regularly and critically reviewing this

9. What is it like to be …. a golden retriever with Duchenne? an MDX mouse? a marmoset with Parkinson’s? a rat with schizophrenia? a geriatric mouse? a diabetic rat with peripheral neuropathy?

10. Predicting adverse effects EyesSome patients have a squint, subnormal binocular vision, or are long sighted. No treatment other than glasses in some cases is required. Unlikely to be a problem to mice. EarsHyperacusis (startled by and sensitive to loud noises). Can become distressed if continuous e.g. washing machine. Mice could have this phenotype. Care taken not to distress them by loud noises. Isolate them from areas where noisy machinery is employed. MouthTeeth of abnormal shape and widely spaced, mainly seen in milk teeth. Do not present problems in eating. They can eat hard food, e.g. apples, easily. Sometimes difficult to wean babies onto solids, but eventually they eat normally. This is not due to their teeth. Unlikely to be a problem to mice but feeding habits of the WS mice will be monitored and mashed food will be employed if necessary. Regular monitoring of teeth and clipping of excess growth has proved necessary. Williams Syndrome: humans and mice

11. Animals’ basic needs Safe, predictable surroundings where they can control their environment and take refuge Stable groups for social animals Sufficient space for normal behaviours A suitably stimulating environment that encourages species-specific behaviours (which also requires space)

12. Noise stress Noise from human activity induces behavioural and physiological stress responses in rodents  morphology of intestinal mucosa  porphyrin staining  changes in behavioural test paradigms Working practices can make a significant difference, e.g. 10 to 15 dB Castelhano-Carlos & Baumans (2009) Laboratory Animals 43: 311-327 Voipio et al. (2006) Laboratory Animals 40: 400-409

13. Enrichment effects Analgesic self-administration study  C57BL mice  singly vs. groups of 3  litter vs. litter, nesting material & gnaw sticks Social housing and enrichment both reduced the need for post-op pain relief Pham et al. (2010) Physiology & Behavior doi:10.1016/j.physbeh.2010.01.038

14. Welfare assessment Aim is to be consistent and objective  www.mousewelfareterms.org A “team” approach  including input from animal technologists and care staff, researchers, veterinarians Objective welfare indicators Appropriate recording systems, e.g. “score sheets”

15. Issues with welfare assessment Many species are adapted to conceal signs of suffering Nocturnal rodents usually observed when they should be asleep Lack of awareness of what to look for and where to look

16. Ongoing research... “Flank twitch” post laparotomy  www.ahwla.org.uk Rodents with no apparent cancer pain self-administer analgesics Many observers concentrate on animals’ faces

17. Principles of effective WA Understanding of good welfare, species- specific behaviour and the “normal” animal  e.g. mice with analgesia post-surgery make well structured nests and defaecate outside them; mice without analgesia do not Arras et al. (2007) BMC Vet. Res. 3: 16 www.biomedcentral.com

18. Keeping pace with new developments All establishments need a formal mechanism for retrieving and assessing information  Ethical Review Process (ERP)  refinement/project based groups  animal care and use committees Part of the commitment to reducing harms, improving welfare and improving science — and reassuring patients

19. Thank you! www.rspca.org.uk/researchanimals