1. Parallel and Simultaneous Dispensing of Nanoliter Volumes for the Production of BioChips and Diagnostic Tests Bellows Dispensing Technology

2. Bottom valves are opened Dispenser is ready for dispensing Top valves are closed Bellows are filled and expanded Connections to individual reagent reservoirs Bottom valves Top valves Bellows Dispenser needles

3. bottom valves opened Lilliput Plate is positioned top valves are closed Bellows are filled and expanded Connections to individual reagent reservoirs

4. bottom valves opened Dispensing starts top valves are closed mechanical compression of the bellows Connections to individual reagent reservoirs

5. bottom valves opened trackdown of the Lilliput chip Connections to individual reagent reservoirs Dispensing ready top valves are closed mechanical compression of the bellows

6. bottom valves opened Removal of the Lilliput chip top valves are closed mechanical compression of the bellows removal of the Lilliput chip Connections to individual reagent reservoirs

7. Valve operation top valves are closed Connections to individual reagent reservoirs bottom valves are closing

8. Valve operation top valves are closed Connections to individual reagent reservoirs

9. bottom valves are closing Valve operation top valves are closed Connections to individual reagent reservoirs

10. bottom valves are closing Valve operation top valves are closed Connections to individual reagent reservoirs

11. bottom valves closed Valve operation top valves are opening Connections to individual reagent reservoirs

12. bottom valves closed Valve operation top valves are opening Connections to individual reagent reservoirs

13. bottom valves closed Valve operation top valves are opening Connections to individual reagent reservoirs

14. bottom valves closed Bellows ready for refill top valves are open Connections to individual reagent reservoirs

15. bottom valves closed Bellows ready for refill top valves opened Connections to individual reagent reservoirs

16. bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand

17. bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand

18. bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand

19. bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand

20. bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand

21. bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand

22. bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand

23. top valves are closing bottom valves are opening Valve operation Connections to individual reagent reservoirs

24. Valve operation Connections to individual reagent reservoirs top valves are closing bottom valves are opening

25. bottom valves opened Dispenser ready for operation top valves closed Connections to individual reagent reservoirs

26. Specifications Accuracy:VC < 2% Volumes:200nl up to 2000nl [2µl – 100µl] Bellows size:3µl up to 2ml Channels:1 to 384 in parallel Reagents:1 to 192 different solutions simultaneously Operation Modes:- constant dispensing from reservoirs - aspirate and dispense Outlet:- stainless steel needles (teflon or paralene coated) - pipette tips Movement Control:Stepper motor. Resolution 14,9 steps to squeeze 1 nl References:- manufacturing of 384 well plates - manufacturing of Lilliput Chip - manufacturing of 96 well Micro Titer Plates

27. Results of 30 doses per each channel: 123456789101112 A 497496490518504 515494500495515499ave. / nl 3.72.31.22.13.71.42.31.01.1 2.52.4STD / nl B 491489511497490489496503493517497506ave. / nl 1.81.11.73.30.91.60.81.31.01.31.21.4STD / nl C 497510495502498497501504501505514509ave. / nl 2.70.92.65.02.32.72.42.20.93.63.41.5STD / nl D 486493502491511497 504516501519512ave. / nl 4.80.73.40.93.11.82.60.92.21.01.61.3STD / nl E 504485492511482517492508501505519508ave. / nl 5.34.70.71.73.41.11.70.92.70.72.82.4STD / nl F 495509519514485496489493503502505487ave. / nl 0.81.14.11.51.22.51.52.32.95.01.41.3STD / nl G 493488482493489478495484494497498493ave. / nl 3.05.41.91.11.32.21.31.04.10.81.04.0STD / nl H 473476474475480472496476478474484481ave. / nl 1.91.81.01.61.54.71.21.41.61.73.31.6STD / nl Validation Data