1. Slide 1Reaction Analytics Inc. ©2011 “iFlow” Flow Analytics System Reaction Analytics Inc. Makers of the iChemExploreriChemExplorer

2. Slide 2Reaction Analytics Inc. ©2011 Flow Chemistry Opens Doors Scale-up microwave chemistry –Similar temp (250 C) under pressure Manage hazardous reactions –Dilute products in “closed” volume Perhaps a path to “new” chemistry –“Click Chemistry” –Reaction under Critical Fluid conditions

3. Slide 3Reaction Analytics Inc. ©2011 Flow Chemistry Pitfalls Heterogeneous Chemistry Precipitates Addition and removal of catalysts Dilute reaction concentration Variations in Reactor Design to accommodate chemistry requirements And more you can think of…

4. Slide 4Reaction Analytics Inc. ©2011 How Flow Can Work Discrete – Reaction Segments Reaction Solvent as Mobile Phase Components mixed on sample tray Mixture injected to Solvent Flow Fluorescent Spacer between segments Continuous – reagents flow with the solvent directly through the reactor Spacer Rxn Segment Spacer Rxn Segment

5. Slide 5Reaction Analytics Inc. ©2011 Flow Chemistry What If’s …Workflow could support process screening AND continuous production …Samples with analytical results are available for every reaction …Hardware and software are robust and serviceable …the Flow Reactor is easily optimized to the chemistry

6. Slide 6Reaction Analytics Inc. ©2011 iChem for Batch iChemExplorer uses vials as reactors and the HPLC to automate sample prep, collection and analysis

7. Slide 7Reaction Analytics Inc. ©2011 FlowChem System Diagram Reactor Section With fittings To Sample Collection To Analysis By LC/MS HPLC Autosampler With ICEbox to Prepare Reaction Segments Reaction Solvent HPLC Pump

8. Slide 8Reaction Analytics Inc. ©2011 FlowChem System Reaction Solvent Pump Autosampler (1367) Column Compartment Detector Analytical Solvent Pump Column Compartment Detector Mass Spec Fraction Collector X Flow Reactor All Boxes in Bone are Agilent HPLC modules Reaction SideAnalytical Side iChemExplorer

9. Slide 9Reaction Analytics Inc. ©2011 Use of Agilent HPLC Modules Control iFlow system with methods and sequences in Agilent Chemstation v.03.01 or higher Mix and match modules BUT compatible firmware is required in all modules on CAN See Agilent Service for parts and modifications

10. Slide 10Reaction Analytics Inc. ©2011 Note on Flow Reactor Keep it Simple –Tube with HPLC fittings wound on a spindle –Spindle with tube on a temperature-controlled Hotplate iFlow can communicate with the IKA Hotplate to control temperature and record with analytical results Example of Accendo Reactor

11. Slide 11Reaction Analytics Inc. ©2011 iFlow Advantages iFlow automates prep of reaction segments HPLC pump delivers 600 bar (9000 psig) to clear obstructions iFlow publishes analytical results for immediate viewing and to Microsoft Excel for presentation and storage to e-lab notebook

12. Slide 12Reaction Analytics Inc. ©2011 Thanks for your attention Makers of the iChemExplorer, Reaction Analytics Inc. now offers the iFlow for optimization of flow chemistryiChemExplorer To learn more please contact us at… administrator@reactionanalytics.com