1. Roles of metabolites in muscle fatigue OBJECTIVES Methodology:Methodology: Experimental approachExperimental approach TemperatureTemperature Are the changes in metabolites responsible for the decrease in force during fatigue at the level of the sarcomereAre the changes in metabolites responsible for the decrease in force during fatigue at the level of the sarcomere

2. INTRODUCTION

3. ATP PRODUCTION – METABOLIC PATHWAYS ATP HYDROLYSIS CREATINE PHOSPHATE PURINE CYCLE ADENYLATE KINASE ATP + H 2 O  ADP + Pi + H + + WORK PCr + ADP  ATP + Cr 2 ADP  ATP + AMP AMP + H 2 O + H +  IMP + NH 4 + IMP + ASPARTATE + GTP  ADENYLSUCCINATE + GDP +Pi ADENYLSUCCINATE  AMP + FUMARATE

4. ATP PRODUCTION – METABOLIC PATHWAYS GLYCOGENOLYSIS AND GLYCOLYSIS KREB’S CYCLE & OXYDATIVE PHOSPHORYLATION GLUCOSE + 2 ADP + 2 Pi  2 ATP + 2 PYRUVATE GLUCOSE + 6 O 2 + 36 ADP + 36 Pi  36 ATP + 6 CO 2 NO O 2 2 LACTATE O 2  -OXIDATION FATTY ACID  ACYL-CoA  KREB’S CYCLE & OXYDATIVE PHOSPHORYLATION GLUCOSYL + 3 ADP + 3 Pi  3 ATP + 3 PYRUVATE

5. FATIGUE: FAST & GLYCOLYTIC TWITCH MUSCLE

6. FATIGUE: SLOW & OXIDATIVE TWITCH MUSCLE

7. ATP EFFECT ON MAXIMUM SHORTENING VELOCITY

8. ATP EFFECT ON MAXIMUM FORCE

9. DISCUSSION

14. CONTROL EARLY FATIGUE FATIGUE Mg 2+ 111 MgATP6.184.74.7 ADP total 0.050.70.7 AMP total 0.00020.0620.062 P i total 0.8817.3817.38 PCr14.161.421.42 Creatine11.4621.1447.3 pH7.007.006.65 ALL CONCENTRATION IN mM

16. Δ DURING FATIGUE F MAX Ca 50 H+H+H+H+↑↑↓↓↓↓ PiPiPiPi↑↑↓↓↓↓ ATP↓↑↓ ADP↑↑↑ AMP↑↑↑ IMP↑↑↑ PCr↓↓↑↑ Creatine↑↑↓↑ Lactate↑↑→→

22. 0% CO 2 WATER 15% CO 2 30% CO 2