1. Arranged by: 1.Nur Laely Mubarokah 2.Lita Purnamasari 3.Andhis Exsa Seftilian 4.Anindita safitri 5.Rizqi Nur Amalia

2. Urban and farm sewage flow into coastal areas and estuaries. These waste waters, which are not always well depurated, contain many bacteria, some of which are fecal and pathogenic for man. After being discharged into the sea, some of these sewage bacteria are diluted and carried seaward.

3. estuarine system is extremely complicated and dynamic.

4. Plummer et al.(1987) clearly indicate that three groups of bacteria occur in estuarine waters : (1) free-living bacteria (2) bacteria attached (3) bacteria able to settle

5. The abundance and occurrence of a special group of bacteria, capable of solubilizing or mobilizing insoluble phosphates were studied in estuarine environment, especially in sediments ( A. Mukherjee, 2011)

6. The estuarine part of the River Ganges, is one of the most dynamic, complex and vulnerable bioclimatic zones in a typical geographical location in the coastal region of Bay of Bengal. Samples were collected from 15 stations along there And all of the stations have many microbes which living there

7. Serial dilution of the samples were made with sterile water of appropriate salinities and plated in sterile petridishes containing Pikovskaya’s Agar

8. Present investigation reveals that phosphate solubilizing bacteria and phosphatase activity exists in estuarine.

9. Vibrio cholerae, Salmonella and Escherichia coli (Enterobacteriaceae) also indicated on estuary, it based on Colwell eksperiment on 1983.

10. the estuary have large populations of sulfide-type magnetic bacteria such as Aquaspirillum serpens, A. dispar and A. itersoni (Dr. Richard P. Blakemore, 1990)

11. Phylogenetic analysis of 16S rRNA genes using denaturing gradient gel electrophoresis (DGGE) The technique for monitoring microbial diversity

12. The goals are: (1)Understand the geographic pattern of sediment bacterial community in different layers along estuary; (2) Identify the major bacterial phylogenetic groups and their diversity based on culture independent; and (3) Assess the impacts of environmental changes such as nutrient variations on the microbial community structure using multivariate analysis.