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Biosensor M. Fatih Abasıyanık.

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1 Biosensor M. Fatih Abasıyanık

2 Biosensors are actively used in science
Self-Monitoring of Blood Glucose with Glucose Sensor has a market (8.8 Billion Dollars in 2008) Biosensor :

3 Aim: Understand the parts of biosensors How they work
To be able to discriminate biosensors according to their quality To be able to design new biosensors To be able to discuss the papers about biosensors To be able to improve biosensors To be able to classify results of sensors.

4 Contents Introduction to Sensors Transduction Elements
Sensing Elements Performance Factors Electrochemical Sensors and Biosensors Photometric Applications Mass-Sensitive and Thermal Sensors Specific Applications


6 Sensor? A device or organ
To detects certain external stimuli and responds in a distinctive manner

7 Basic sensors An acid turns blue litmus paper red and a base turns red litmus paper blue

8 pH meter

9 Types Physical sensors: measure Chemical sensors: measure
Distance Mas Temperature Pressure Chemical sensors: measure Chemical substrances by chemical or physical response Biosensors: measure Chemical substrances by using a biological sensing element. All of them  connected to a transducer visible response occurs

10 Chemical Sensors and biosensors detect chemicals called analyte.
If analyte is a biomolecule, the device can be called biosensor Recognation element

11 Types of transducer Electrochemical biosensor: mostly used Optical
Potentiometric: measure potential Voltammetric : an increasing (decreasing) potential is applied to the cell until Ox. (re.) of the substrance to be analysed occure and a sharp rise (fall) in the current to give a peak. Conductometric FET based Optical Piezo electric Thermal

12 İmmobilization

13 Performance factors Selectivity Sensitivity Accuracy Response time
Recovery time Lifetime


15 Biosensor: Biorecognation Unit (Sensing Element; biologic detection unit)

16 Sensing Elements It is concerned with various ways in which a sensor can recognize an analyte. specific for that analyte alone, Selective ;responding to the required analyte more than to other species. Types of Sensing Elements İonic (chemical sensors) Molecular (chemical sensor and biosensor) Biological (biosensor)

17 A- Ionic Recognation: Ion-Selective Electrodes
Possible to be used in biosensors. Example: urea  ammonia They are based on the principle of the emf of a concentration cell. A potentiometric device in which the change in emf is proportional to the logarithm of the analyte concentration. The selectivity by the membrane separating the analyte solution from the internal reference solution. 0.5 0.0

18 The level of interference is measured by the selectivity coefficient
Interferences Sensors respond to one ion more than to others, although there is often a small response to unwanted ions. This is known as interference. For example, a fluoride ISE responds to hydroxide at one tenth of the response level (to fluoride) for equal concentrations of the ions. The level of interference is measured by the selectivity coefficient kij=the selectivity coefficient. 0.1 0.5 0.0 ISAB (ionic-strength adjustment buffer): [OH] declined to 10-9M pOH:9 . it is lower than detection limit for OH

19 A- Ionic Recognation: Conducting Devices
ionsconductor for electreicty quantity measurement resistance (1/conductivity) conductance depends on…..; degree of ionization (storng or weak) charge of ion number of ions mobility of ions (related to size, smallfastermore conductive) conductivity not used alone ion chromography (extensivily)

20 A- Ionic Recognation: Modified electrodes
selectivityimportant electrodes not selective alone to gain a selectivitymodify them two ways modify themselves modify its surface coated with polymers best exampleCPE(Carbon paste electrode) suitable electrodegraphite powder with Nugola stiff paste   mix with a modified component (electroactive;such as ferrocene) or a complexing agent extracting electroactive analyte into the surface of the paste

21 A- Ionic Recognation: Modified electrodes
modify electrode surface by coating with different types of polymers Mainly 3 types conducting polymers ion-exchange polymers redox polymers a) Conducting polymers most studied ones: polyacetylene, polypyrrole, polyaniline and polythiophene easy to polymerize: electrochemically oxidizing the substrade on the electrode. solvents and counter ions effect on the polymer

22 B- Molecular Recognition: 1. Chemical Recognition Agents
Thermodynamiclyrxn to be controlled M= analyte L=Recognition Agent M-L complex, or M or L respond to a particular transducer (optical, electrochemical etc) Optical response: an absorption or fluorescence change to the analyte PVC (polyvintylchloride) successfully used for such biosensor (ion- selective electrodes also) Potentiometic e. (used)

23 Some Application with such recognitin agents
Analyte: Iron (II) Recognation Agent: 2,2’-bipyridyl in a poly (vinyl pyridine) (PVP) membrane. Method: iron(II) oxidized by linear-sweep voltametry. Complexation rxn (M-L) accumulation of iron İndicators can be used to analyze the analytes. Optodes: Optical biosensors (colorimeter or spectrophotometer)…. Used to detect the colour change

24 Molecular Size: If the analyte is smaller than the others in the sample, size is selective and molecular sieves are used as recognition agent. Examples: The antibiotic, valinomycin is a neutral ionophore Making complex with potassium ions selectively K fits into its cavity Valionmycin used as a recognition reagent to detect K

25 B- Molecular Recognition: 2. Biological Recognition Agents
Biological systemsthe major selective elements They must attach themselves to one particular substrate, but not to others. Enzymes Antibodies Nucleic acids Receptors Affinity biosensors: Sensors with antibodies of receptors as Recognition Elements

26 1. Enzymes Free (purified) Enzmyes Tissue Materials Micro-organisms
Organelles (Mitochondria)

27 Enzymes A. Purified Enzyme
Many enzymesoxidation-reduction (electrochemically) S:substrate ; E: Enzyme; ES:enzyme-substrate complex; P:product

28 Michaelis-Menten Equation
enzymes Michaelis-Menten Equation

29 enzymes

30 Double-reciprocal plot
enzymes Double-reciprocal plot

31 Advantages and Disadvantages of using Enzymes in sensors
Highly selective Catalytically active improve sensitivity Fairly fast-acting One of the most known biological components Expensive A loss activity when immobilized on a transducer Tending to lose activity after a relatively short period time

32 Tissue multiplicity of enzymes
B. Tissue Materials: Tissue multiplicity of enzymes Thus, not selective as purified enzymes But, longer life protected (in its environ.) Response slow (diffusion) Cheaper Example: Banana tissue sensor: [Eggins (1997)] Banana biosensor

33 İts derivatives: flavanols found in beers and wine
enzymes For dopamine, a catcholamine found in brain containing a complex of polyphenolases which catalyse the oxidation of polyphenolic compounds İts derivatives: flavanols found in beers and wine Polyphenol oxidase catalyse the rxn. İt is found in banana

34 Advantages: Disadvantages: Enzyme in natural environment
enzymes Advantages: Enzyme in natural environment Activity stable Less expensive They sometimes work when purified enzymes fail Disadvantages: Loss selectivity Diffusion problems

35 C. Microorganisms: They play important role in
enzymes C. Microorganisms: They play important role in Brewing Pharmaceutical synthesis Food manufacture Waste-water treatment Energy production MO immobilized to transducer directly MO can ….. Assimilate organic compounds Produce electroactive metabolites

36 Advantages: Disadvantages: Cheaper
enzymes Advantages: Cheaper Less sensitive to inhibiton by solutes, more tolerant to pH & temperature Longer lifetimes Disadvantages: Longer response time Longer recovery activity times Many enzymes and less selectivity

37 D. Mitochondria (organelles)
enzymes D. Mitochondria (organelles) Sub-cellular multi-enzyme particelscatalyze an analyte

38 Versitile (large spectrum) Possible to develope for any antigen
antibodies 2. Antibodies: Versitile (large spectrum) Possible to develope for any antigen İts function: to bind an invading antigen and remove it from harm

39 Heavy chain hinges Fab: antigen-binding fragment light chain Fc: crystalizable F Gerald edelman & Rondey porter

40 Labelling Ab Mass change etc also can be seen Radioisotopes Enzymes
antibodies Labelling Ab Radioisotopes Enzymes Red cells (RBC) Fluorescent probes Chemiluminescent probes Metal tags Mass change etc also can be seen

41 Advantages: Disadvantages. High selectivity Ultra sensitive
antibodies Advantages: High selectivity Ultra sensitive Bind very powerfull TNT also can be detected by antibodies R.D. Shankaran, K.V. Gobi, K. Matsumoto, T. Imato, K. Toko and N. Miura, Highly sensitive surface plasmon resonance immunosensor for parts-per-trillion level detection of 2,4,6- trinitrophenol, Sens. Actuators B: Chem. 100 (2004), pp. 425– 430. Disadvantages. No catalytical

42 DNA assays can involve the addition of labelled DNA to the system
nucleic acids 3. Nucleic Acids: like antibodies DNA probesused to detect genetic diseases, cancers and viral infections DNA assays can involve the addition of labelled DNA to the system Radioactive Photometric Enzymic Electroactive

43 Examples to NA biosensor
nucleic acids Examples to NA biosensor

44 The response may be either …
4. Receptors: A receptor is a structure in a cell, which can trigger an amplified physiological response when it is bound to a particular ligand (an agonist). An agonist a chemical binding to a receptor and triggers a response by the cell. An agonist often mimics the action of a naturally occurring substance. An agonist produces an action. An antagonist blocks an action of an agonist. The response may be either … ion-channel opening, production of second messenger systems, or activation of enzymes.

45 Recepors tend to have an affinity for a range of structural related compounds.
Like others, also they are labelled Examples: Biosensor for Anaestherics

46 Transducers

47 Types of transducers Electrochemical Transducers
Potentiometric Voltammetry and Amperometric Conductivity Field Effective Transistors Photometric Sensors Others

48 Electrochemical Transducers


50 metal (such as silver) is placed in a solution containing ions
(such as silver ions), there is a charge separation across the boundary between the metal and the solution. an electron pressure, usually termed a porential. It cannot be measured directly, and requires two such electrode-electrolyte combinations. Each of these is called a half-cell. Such a combination is called an electrochemical cell

51 Half cells conntected by two ways
internally by means of an electrically conducting bridge or membrane. Externally by a potential measuring device, such as a digital voltmeter (DVM). Wire has a very high internal impedance, such that very little current will flow through it. The electrical circuit is now complete and the emf of the cell can be measured. This value is the difference between the electrode potentials of the two half-cells. Depends on a number of factors, (i) the nature of the electrodes, (ii) the nature and concentrations of the solutions in each half-cell, (iii) the liquid junction potential across the membrane (or salt bridge).


53 Other Reference Electrodes
SHE is a reference electrode (RE) to which other electrodes may be referred. While it is not difficult to set up an SHE in the laboratory, it is not very convenient for routine measurements as such an electrode involves flowing hydrogen gas, which is potentially explosive. Other secondary reference electrodes are therefore used Easy set up Non-polarizable Reproducable electrode potential Many varieties, 2 are in common

54 Silver chloride soluble
İt consists of a silver wire coated with silver chloride dipping into a solution of sodium chloride Calomel means mercurous chloride (Hg2Cl2). A mercury pool in contact with a paste made by mixing mercury chloride powder and saturated potassium chloride


56 ion-selective electrodes (ISE)
left test solution (a reference electrode dipped into). The ion-selective membrane is in the middle (dividing the test solution from the standard solution, with a fixed concentration of the ions being measured. A second reference electrode is dipped into standard solution is placed. The two reference electrodes are connected through a high impedance voltmeter - usually a digital voltmeter. The electrode system is then usually calibrated with standard solutions in one of a number of ways, as to be described..


58 An ISE is an ion-selective electrode, designed to respond to one particular ion more than others.
A potentiometric device: the potential of the electrode (measured against an appropriate reference electrode) is proportional to the logarithm of the activity (or concentration) of the ion being tested. Such a device usually responds rapidly, with a linear range of about 10-6 to 1 M for most ISEs. It operates on the principle of a concentration cell, in that it contains a selective membrane which develops a potential if there is a concentration difference across the membrane of the ion being tested.

59 Precautions for ISE İonic strengtconstant pH controlled
Add components to Minimize or eliminate interfering ions. ISA (ionic strength adjusters) or TISABs (total- ionic-strength adjustment buffers) present

60 Measurement and Calibration
Calibration Graphs and Direct Read Preapare a series of standards Read them Read the sample Make a plot (log[concentration] versus potential) Estimate

61 Measurement and Calibration 2. Standard Addition:
Prepare a standard whose concentreation is probabily 10 times higher than sample Read sample Add stanadrd to sample and read it Use the following formula to estimate the concentration

62 Measurement and Calibration
3. Gran plot: Similar to standard addition method Series of standard prepared Add them to sample and read



65 Electrochemical Transducers
2. Amperometric



68 2. Linear Sweep Voltammetry Voltammetry:information about an analyte is obtained by measuring the current as the potential is varied. 1) linearly varying potential between a working electrode and a reference electrode in 2) an electrochemical cell containing a high concentration of an indifferent electrolyte to make the solution conduct - called the supporting electrolyte and 3) an oxidizable or reducible species - the electroactive species. Current monitored Potential against currentploted =voltammogram This techniqulinear sweep voltammetry (LSV) LSV is a voltammetric method where the current at a working electrode is measured while the potential between the working electrode and a reference electrode is swept linearly in time. Auxillary (supporting) electrode Reference electrode working electrode

69 A :current very small A-B: becouse of impurities (background current) B: potenital approaches the reduction potential of the oxidized species. B-C: higher potential electrons from the electrode to the Ox at increasing rate The increase in reduction rate  cell current to increase inet (cell current) ic (cathodic-reduction- current) İa (anodic-oxidation current)

70 E ↑ic ↑ but ia ↓ Rise in voltammetric wave At C: [Ox] is limited
İt is depleted by reduction Rate of fresh Ox from bulk of solution A peak in current Id: diffusion limited current -IL

71 But we will need high matematical calculations
We have another straightforward solution We have current at the point C in our hand How we can find this to calculate [analyte]


73 3. Cyclic Voltammetry Why: to understand the mechanism of Redox rxn
How works: again 3 electrodes like voltametry What the difference is Potential reversed so reverse everything (if rxnreversible

74 While Ox at the electrode surface depleted by the reduction replaced by the Red. (diffuses into the solution) Hence, if we reverse the potential sweep from the positive side of the peak, we shall observe the reverse effect. As the potential sweeps back towards the redox potential, the reduced species will start to be re-oxidized to Ox. The current will now increase in the negative (oxidizing) direction until an oxidation peak is reached. two peaks: 1st: the reduction of the original substrate 2nd: reoxidation of the product back to the original substrate. peak currents almost identical heights. The average of the two peak potentials is equal to the standard redox potential, regardless of the concentration of substrate or its diffusion coefficients or rates of electron transfer.

75 4. Chronoamperometry Instead of sweeping the potential, the latter is stepped in a square-wave fashion to a potential just past where the peak would appear in linear-sweep voltammetry. The current is then monitored as a function of time. Decay occurs because of the collapse (or spreading out) of the diffusion layer

76 decay is proportional to the reciprocal of the square root of time, as shown by the Cottrell equation: Chronoamperometry can be used to determine any of the variables in the above equation, providing that the others are known. A,C,D or n

77 Constant related to the diffusion layer thickness
5. Amperometry usual name for the analytical application of the chronoamperometric technique. With certain cell and electrode configurations, the decaying current reaches an approximately steady state after a certain time. This is shown by the shaded part of the curve in the figure left The current has become effectively independent of time, as indicated by the following equation: Constant related to the diffusion layer thickness


79 6. Conductivity inverse of resistance
A measure of the ease of passage of electric current through a solution. Ohm’s law E=I/R ; L=1/R and therefore E=I/L The conductivity varies according to the charge on the ion, the mobility of the ion the degree of dissociation of the ion.

80 impedance: conductance, capacitance and inductance  attantion
In principle, a change in conductance can be used to follow any reaction that produces a change in the number of ions, the charge on the ions, the dissociation of the ions or the mobility of the ions. Usually a differential type of cell is used, as shown in the following Figure.

81 7. Piezo-Electric Effect
Principles In 1880, The Curie brothers discovered “ 1. anisotropic crystals give out an electrical signal when mechanically stressed.” 2. if an electrical signal is applied to such crystals, they will deform mechanically (With the application of an oscillating electrical potential, the crystal will vibrate).

82 Every crystal has its own natural resonant frequency of oscillation, which can be modulated by its environment. The usual value of this frequency is in the 10 MHz region, i.e. radiofrequency. The actual frequency is dependent on the mass of the crystal, (any other material coated on it) The change in resonant frequency (Af) resulting from the adsorption of an analyte on its surface can be measured with high sensitivity ( Hz g-’), when applied in sensors can thus result in devices with pg detection limits. The relationship between the surface mass change, Δm, and the change in resonant frequency, Δf, is given by the Sauerbrey equation, as follows: Δm:the mass in grams of the adsorbed material on an area A (cm2) of the sensing region, Δ f : the overall resonant frequency. For a 15 kHz crystal, a resolution of 2500 Hz kg-l is likely, so that a detection limit of g (1 pg) is achievable. Materials showing the piezo-electric effect  ceramic materials such as barium and lead titanates Some organic polymers, such as poly (vinylidene fluoride) (PVDF) (-CF2-CH2-CF2-),, also form crystals with piezo-electric properties.

83 The frequency at which an oscillator works is usually determined by a quartz crystal. When a direct current is applied to such a crystal, it vibrates at a frequency that depends on its thickness, and on the manner in which it is cut from the original mineral rock. Some oscillators employ combinations of inductors, resistors, and/or capacitors to determine the frequency. However, the best stability (constancy of frequency) is obtained in oscillators that use quartz crystals

84 3. Photometric Sensors Abasıyanık M. F.

85 Most bioassays photometric type
How workChanges in species with photometric properties. Example:

86 The problem: how to make a sensor by using an optical technique.
The basic optical response is based on the Beer-Lambert law (usually referred to as Beer’s law), as follows: IO: the intensity of the incident light; I: the intensity of the transmitted light; A: the absorbance (usually measured directly by an instrument), ε: extinction coefficient, C : the concentration of the analyte, L: the pathlength of light through the solution. (result in limitation of size of sensors, which does not occur in electrochemical devices)

87 What are the advantages of optical sensors?
No ‘reference electrode’ is needed, although a reference source is often useful. There is no electrical interference. An immobilized reagent does not have to be in contact with any optical fibres, and can easily be replaced. There are no electrical safety hazards. Some analytes, such as oxygen, can be detected in equilibrium. They are highly stable with respect to calibration, especially if the ratio of the intensities at two different wavelengths can be measured. They can respond simultaneously to more than one analyte by using multiple immobilized reagents with different wavelengths for response, e.g. 02 and CO. Multi-wavelength measurements can be made to monitor changes in the state of the reagent. They have potential for higher-information content than electrical transducers.

88 What are the disadvantages of optical sensors?
They will only work if appropriate reagent phases can be developed. They are subject to background ambient light interference. This may be excluded either directly or by using a modulation technique. They have a limited dynamic range when compared to electrical sensors – typically 102 compared with 106 – for ion-selective electrodes. They are extensive devices, and dependent on the amount of reagent, and hence difficult to miniaturize. There are problems with the long-term stability of the reagents under incident light. Response times may be slow because of the time of mass transfer of analytes to the reagent phase.

89 Ultraviolet-visible absorption
Optical Techniques Main types of photometric behaviour in biosensor applications: Ultraviolet-visible absorption Fluorescence (and phosphorescence) emission Bioluminescence Chemiluminescence Internal reflection spectroscopy (IRS) Laser light scattering methods


91 1. Ultraviolet and Visible Absorption Spectroscopy
The absorbance is measured by passing incident radiation through the monochromator of a spectrometer and then measuring its intensity with a photomultiplier or photodiode, thus producing an electrical signal which is proportional to the absorbance at a particular wavelength. The measured absorbance is linearly proportional to the concentration. However, the apparatus relatively cumbersome Expensive,


93 2. Fluorescence Spectroscopy (animation)
Species absorb light, the excited species will decay in one of a variety of ways. decay to the lowest excited singlet state, re-emit radiation, usually at a lower wavelength than the original excitation. This phenomenon is known as fuorescence, Emitted light can be measured

94 Turn off light

95 Quenching refers to any process which decreases the fluorescence intensity of a given substance. 
The excited molecule transfer its energy to the new one (quenching)



98 Luminescence Emission of light Chemiluminescence : certain chemical RXN emission of light without heat – Bioluminescence: The similar emission of light from biological systems.

99 3. Chemiluminescence, As a result of the oxidation (with O2 or H2O2) of certain substances (example:luminol), visible light 'in the cold‘ and in the absence of any exciting illumination.


101 particularly useful with immunoassays Example:
Luminol is normally used as a label and employed in any assay involving; oxygen, hydrogen peroxide or peroxidase. particularly useful with immunoassays But sensitivity is limited because the quantum yield is only 1%. Example: A particularly interesting approach, combining both luminescence and fluorescence An antigen  labelled with the luminol, while the corresponding antibody labelled with a fluorescent compound. the emission from the luminol will excite the fluorescence. Ag-Ab: luminol emits light of 460 nm, which excites the fluorescor, İt emits fluorescence in turn at 525 nm, thus results in an increased quantum yield. At the same time, unlabelled antigen combines with labelled antibody, in which case there is no fluorescence but emission luminol at 460 nm  permits analysis of both bound and unbound.

102 Their usage in Biosensors Examples:
A photodiode is a type of photodetector capable of converting light into either current or voltage, depending upon the mode of operation Their usage in Biosensors Examples: luminol with hydrogen peroxide and peroxidase. A fibre-optic sensor for H202 : peroxidase immobilized on PAGE (polyacrylamide gel) + luminol at the end of the fibre. The luminescence is detected in situ no external light source is needed. The sensor can be connected directly to the photodiode. It will detect 1-10 mM H202, with a response time of 2 min. One obvious application is to 'connect' the sensor to a glucose-glucose oxidase reaction system to determine glucose, for which a linear concentration range of mM can be obtained.

103 A recent example: adamantyl dioxetine phosphate adamantyl dioxetine anion (by alkaline phosphatase ), unstable fluoresces. fluorescence lifetime is several minutes, unlike conventional luminescence Such behaviour could be used in many types of assay which involve phosphate ester hydrolysis using alkaline phosphatase. adamantyl dioxetane phosphate compete with other organic phosphates for the phosphatase.

104 4. Bioluminescence Certain biological species (firefly) emit luminescence. This originates in a group of substances of varied structures known as luciferins. The enzyme-catalysed oxidation of luciferin results in luminescence.

105 Rxnvery sensitive down to femtomole [10-15 ] example:
determination of creatine kinase related to myocardial infarction and muscle disorders Bacterial luciferases do not use luciferins but .. Most analytical reactions  NADH for FMN to FMNH2 Example:

106 alcohol dehydrogenase
NADH oxidationfor ethanol determination alcohol dehydrogenase


108 In most photometric methods:
Advantages of luminescence methods when compared with other photometric methods In most photometric methods: comparison of light absorbed in the presence of the analyte with the corresponding light in the absence of the analyte,(which will not usually be zero). Luminescence (chemiluminescence- bioluminescence) is measured against a background of complete absence of light. Much more sensitive detectors much lower levels of light detecteddetection limits very much lower

109 Optice transducers Fiber optic glasses (optic fibers) key components. An optical fiber is a glass or plastic fiber that carries light along its length. Light is kept in the core of the optical fiber by total internal reflection.  Total internal reflection is an optical phenomenon that occurs when a ray of light strikes a medium boundary at an angle larger than a particular critical angle with respect to the normal to the surface.

110 When light crosses a boundary between materials with different refractive indices, the light beam will be partially refracted at the boundary surface, and partially reflected. However, if the angle of incidence is greater (i.e. the ray is closer to being parallel to the boundary) than the critical angle – the angle of incidence at which light is refracted such that it travels along the boundary – then the light will stop crossing the boundary altogether and instead be totally reflected back internally. This can only occur where light travels from a medium with a higher refractive index to one with a lower refractive index. For example, it will occur when passing from glass to air, but not when passing from air to glass air glass

111 Optical fibers regarded as ‘light conductors’ or ‘light wires’.
metal electrical wires conduct electricity (often over very long distances) Optical fibres does the same for light. optical fibres are even for telephone transmission. Optical fibres behave as waveguides for light. Original fibres are made of glass, but now polymeric materials used much cheaper than glass and the metal wires The light waves are propagated along the fibre by total internal rejection (TIR). TIR depends on the angle of incidence and the refractive indices of the media:



114 2.7.7 Device Construction system works better with a ‘reference blank’, with the light source then being split between the sample and the reference. Detection may be effected at different analytical and reference wavelengths and one then obtains the ratio of the signals at the two wavelengths. This eliminates scatter and source fluctuations. Waveguides for different forms of light may need to be made of different materials, as follows: λ > 450 nm, plastic (such as polyacrylamide) λ > 350 nm, glass λ < 350 nm, fused silica λ > 1000 nm, germanium crystal In a photometric sensor, the reagent has to be immobilized so that it can interact with the analyte, probably to form a complex with distinctive optical properties which can then be monitored by the sensor.

115 Solid-Phase Absorption Label Sensors
The criteria for the application of these are as follows: Choice of immobilization support Immobilization of indicators, thus retaining activity in the desired rays Immobilization of biorecognition molecules with retention of activity Cell geometry Choice of source and detector components portable device low-power components (desirable) such as LED (light emitting diode)sources and photodiode detectors. LEDs have only a limited range of wavelengths


117 Performance Factors Chapter III IV. Week



120 Ion-selective Electrodes:
Selectivity Selectivity: the ability to discriminate between different substances. It is a function of the selective component (biodetection element), although sometimes the operation of the transducer contributes to the selectivity. Ion-selective Electrodes: Respond to particular ions BUT, nearly all can respond to some other ions (interference) Interference can be measured and for commercial sensors, it should be given.

121 ki,j:Electrode potential and a selectivity coefficient,
The interference is expressed in the Nicolskii-Eisenman equation ki,j:Electrode potential and a selectivity coefficient, ai : activity of the primary analyte of charge n aj: activity of the interfering analyte of charge z E: potantial of the system ; K and S: konstant

122 ki,j determined by a “two-point, mixed solution method” Example:
Selectivity ki,j determined by a “two-point, mixed solution method” Example: The cell potantial primary analyte (E1) (0.001M) 0.001M (primary analyte)+0.1 or 0.01M potentially interfering ion (E2)

123 Selectivity Question: Transducer: A calcium ISE S: mV/decade in a M solution. E1: mV (in a M calcium chloride solution) E2: mV (mixed sol. M calcium chloride and 0.1 M sodium chloride). Calculate the selectivity coefficient for calcium ions in the presence of sodium.

124 2. Sensitivity Sensitivity

125 How to calculate DETECTION LIMIT: There are many definitions of DL.
2. Sensitivity Range, Linear Range and Detection Limits Sensitivity How to calculate DETECTION LIMIT: There are many definitions of DL. it is important to know .. what concentration range is covered and over what section of this range the response is linear. At the lower level is the detection limit. DL is the concentration of analyte at which the extrapolated linear portion of the calibration graph intersects the baseline – a horizontal line corresponding to zero change in response for several decades of concentration change.


127 Linear Range: should include effective constentration of the target
Sensitivity Linear Range: should include effective constentration of the target For example: for determination of glucose, biosensor’s linear range  cover (0.2-20mM, possible concentration of glucose in blood, normal-diabetic respectively) Potentiometric sensor’s linear range larger pH (H+)-selective electrode’s linear range from ph ( ) Amperometric sensors and biosensors do not ranges have ranges of much more than two or three powers of ten

128 Statistical anaylsis important for bioanalytical chemistry
Sensitivity Statistical anaylsis important for bioanalytical chemistry Mean, standart deviation, CV (Coefficient of Variation), CV = (Standard Deviation / Mean) * 100 Example

129 CV ( )

130 the time that elapses between a stimulus and the response to it.
Response Time 3. Time Factors 3.1. Responce Times Some definations the time that elapses between a stimulus and the response to it. The amount of time it takes for a device to react to an input signal. time to allow the system to come to equilibrium, the response time.

131 Chemical sensors: their response time short
For example: nitrate electrodes  the most reproducible results were obtained after stirring the solution in contact with the electrode for ~30 s. Biosensors: Response times from a few sec.- to a few min. Up to 5 min acceptable >10 min too long. if the time becomes too long it can affect the usefulness of the method for repetitive routine analyses.

132 In many publications, these times (recovery and response) are combined
Recovery Time 3.2. Recovery Times: the time that elapses before a sensor is ready to be used for another sample measurement. It may be immediate after one measurement the sensor system has to rest to resume its base equilibrium before it can be used with the next sample. In many publications, these times (recovery and response) are combined

133 Biosensors and chemical sensor’s lifetimes can be different
The most robust pH electrodes tend to deteriorate after months of use. What does lifetime mean? Types? The response during continous use the sensor is in constant contact with an analyte solution and successive readings are made over a period of, e.g. hours. This lifetime in use can be defined as the time after which the response has declined by a given percentage (say 5%).

134 3. Storage stability: the period when the sensor is stored
lifetimes 2. Shelf life stability: the time over which the assembled sensor is stored, perhaps in a buffer solution or an ISAB (ionic-strength adjustment buffer) 3. Storage stability: the period when the sensor is stored dry in its packing, for an ion-selective electrode, when a biological material is stored separately, perhaps refrigerated. All organic material deteriorates with time, especially when taken out of its natural environment. Biosensor studies to show how the response of the biosensor to a standard sample changes with time over hours, days and even months. Generally pure enzymes have the lowest stability, whereas tissue preparations have the highest.

135 lifetimes Examples to Techniques to improve lifetime of biomaterials of biosensor: Gibson et all in stabilizing a range of enzymes by using a mixture of the followings as additives (stabilizers) a polyelectrolyte (DEAE-dextrin) A sugar alcohol (lacticol) Enhanced the retention of enzyme activity during.. Desiccation Thermal stress They optimized this method on .. Alcohol dehydrogenase Horseradish peroxidase Other scientist perform on 12 other enzymes

136 L-glutamate biosensor (NMP-TCNQ-modified graphite electrodes)
lifetimes Examples: Alcohol oxidase (alcohol determination; with membrane immobilization and horseradish peroxidase (amperometric oxidation of hydrogen peroxide ) with a mediated coupled reaction and N- methylphenothiazine- tetracyanoquino- dimethane (NMP- TCNQ) on a graphite electrode. In both cases, stabilizers promoted a considerable increase in storage stability in the shelf life (dried form; 37°C) Similarly; L-glutamate biosensor (NMP-TCNQ-modified graphite electrodes) L-glutamate oxidase an increase in shelf life Other workers, (just the DEAE-dextran polyelectrolyte) glucose oxidase biosensors, were less successful. In fact, lyophilized glucose oxidase alone is extremely stable, (for 2 years at 0°C and for 8 years at - 15°C) In solution, it is most stable at pH 5, while below pH 2 and above pH 8 the catalytic activity is rapidly lost.

137 Precision and Accuracy
accuracy of a measurement: the degree of closeness of measurements of a quantity  to its actual (true) value precision (reproductibilty) of a measurement system: the degree to which repeated measurements under unchanged conditions show the same results High accuracy Low precision Low accuracy High precision





142 Precision and Accuracy
Standard addition method The method of standard addition is used in instrumental analysis to determine concentration of a substance (analyte) in an unknown sample by comparison to a set of samples of known concentration, similar to using a calibration curve. Standard addition can be applied to most analytical techniques and is used instead of a calibration curve to solve the matrix effect problem.

143 Different Transducer Performance Types of analyte Urea biosensor
Amino acid biosensor Glucose biosensor Uric acid affected by the amount of enzyme Type of immobilization Some examples

144 İn table forward, urea biosensor compared
Cationic (NH4+, pH and gas (NH3 and CO2 ) pH sensor more enzyme needed Below 5mM linear Longest response time Ammonia Gas electrode Best one 4 months stabek Use little enzyme Short response time Cationic Best rsponse time Excellent response range 3 weeks stable


146 Determination of urea with an ammonia gas-sensitive semiconductor device in combination with urease
F. Winquista, A. Spetza, I. Lundströma and B. Danielssonb aLaboratory of Applied Physics, Linköping Institute of Technology, S Linköping Sweden bDepartment of Pure and Applied Biochemistry, Chemical Centre, University of Lund, S Lund Sweden Received 3 April 1984.   Available online 18 January 2002.  Abstract An ammonia gas-sensitive Ir/Pd MOS capacitor is used for urea determinations with the aid of urease in two different systems. One combination utilizes a reaction column with immobilized urease in a flow-injection system. The lower limit of urea detection for 150-μl samples was 0.2 μM. Urea in whole blood and blood serum was determined after a 500-fold dilution, and 15 samples per hour could be assayed. The relative standard deviation was 4.6% (n=10). Recovery tests were satisfactory. Values obtained for urea in serum correlated well with those from a spectrophotometric method. The other combination is based on a small flow cell with free urease enclosed between a dialysis membrane and a gas-permeable membrane. Urea was determined in the concentration range 0.01–50 mM. The enzyme probe could be used for up to four days without changes of behaviour. Urea Biosensor A urea biosensor on an ENFET (urease in sensing gate) pH mM urea 2 weeks storage at 4 degree C Response time 2 min. No interfering with glucosei creatinine and albumine Ir-Pd MOS (metal oxide semiconductor) device Read the abstract

147 2. Glucose Biosensor




151 2 7 2)

152 Health Care Health Care Mesurements of blood, gases ions and metabolites regularly analyzed. Convential methods  slow Chemisensors and biosensorsfast Latest ExacTech® biosensor: in 12 second Problem: sensors for different analytes needs specialist scientist (Nurse) to operate it. BUT, new smart biosensors because of FET technology (field effect transistors) that can combine several measurements in a sensor unit. Sodium, potassium, calcium and pH by one sensor Glucose, lactate and urea

153 Health Care

154 Dream for scientist to produce....
Health Care Dream for scientist to produce.... An implanted biosensor for continuous monitoring of a metabolite HOW Via a microprocessor to a controlled drug-delivery system throug the skin. Diabetes Automatit delivery system gives insulin (artifical pancrease)

155 Control of Industrial Process
Environmental Monitoring:

156 Health Care Health Care Mesurements of blood, gases ions and metabolites regularly analyzed. Convential methods  slow Chemisensors and biosensorsfast Latest ExacTech® biosensor: in 12 second Problem: sensors for different analytes needs specialist scientist (Nurse) to operate it. BUT, new smart biosensors because of FET technology (field effect transistors) that can combine several measurements in a sensor unit. Sodium, potassium, calcium and pH by one sensor Glucose, lactate and urea

157 Dream for scientist to produce....
Health Care Dream for scientist to produce.... An implanted biosensor for continuous monitoring of a metabolite HOW Via a microprocessor to a controlled drug-delivery system throug the skin. Diabetes Automatit delivery system gives insulin (artifical pancrease)

158 Control of Industrial Process
Environmental Monitoring:

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