1. Immune System Objectives – To select genes that are embryonic lethal Or accept bone marrow from other centres – Generate bone marrow chimeras Or generate conditional mutants for EUCOMM allels – Analyse phenotypes of these mutants and Establish the course of embryonic lethality

2. Members of the Network Principal Investigator Werner Muller, University of Manchester, Faculty of Life Science, AV Hill Building, M13 9PT Manchester; Email: Werner.muller@manchester.ac.uk Co-Investigators Immunology: Richard Grencis, Kathryn Else, Mark Travis, Sheena Cruikshank, Douglas Millar, Dr. Mat Hardman, Paul Lyons, Jim Middleton Immune Toxicology: Ian Kimber Neurobiology: Emmanuel Pinteaux, Stuart Allan Cardiology: Ludwig Neyses, Elizabeth Cartwright, Delvac Oceandy Developmental Biology: Mike Dixon, Kathryn Hentges Human Genetics: Yanick Crow Bioinformatics: Andy Brass, Robert Stevens Manchester Centre for Nuclear Hormone Research in Disease: David Ray

3. Example 1 Analysis of a B cell phenotype for an embryonic lethal mutation (polymerase beta)

8. Example2 Analysis of a complex phenotype for an embryonic lethal mutation using conditional gene targeting (gp130)

13. neurological, cardiac, hematopoietic, immunological, hepatic and pulmonary

16. Immune System Objectives – To select genes that are embryonic lethal Or accept bone marrow from other centres – Generate bone marrow chimeras Or generate conditional mutants for EUCOMM allels – Analyse phenotypes of these mutants and Establish the course of embryonic lethality