1. Physiological specialization of Puccinia triticina on wheat in the United States in 2010. Jim Kolmer Dave Long Mark Hughes USDA-ARS Cereal Disease Laboratory St. Paul MN

2. Puccinia triticina 2010 - USA 537 single uredinial isolates 40 virulence phenotypes total MLDS 30% (Lr9, Lr17, Lr39/41) TDBJG 23% (Lr2a, Lr24) TCRKG 13% (Lr2a, Lr26, Lr11, Lr18)

3. Virulence Phenotypes P. triticina 2010 RaceSoutheast (52) Northeast (58) Ohio Valley (57) TX-OK (135) KS-NE (58) MN-SD- ND (149) MCTSB0%16%14%0%2%0% MFPSB10%0%5%7%05% MLDSD39%7%2%48%21%36% TCRKG23%31%56%4%0%3% TDBGG0% 6%9%1% TDBJG14%0%14%27%38%32%

4. Virulence P. triticina 2010 Gene Southeast (52) Northeast (58) Ohio Valley (57) TX-OK (135) KS-NE (58) MN-SD- ND (149) Lr2a42%53%75%42%78%55% Lr939%19%2%50%35%38% Lr160% 3% Lr2433%16%21%42%53%54% Lr2639%74%79%15%9%24% Lr1133%78%74%7%19%4% Lr1752%35%26%57%36%44% Lr210% 8% Lr39/4139%22%2%50%35%38%

5. P. triticina regional populations - 2010

6. Lr21 Virulence - 2010 Collections from MN-ND CultivarMLDSDTFBGGTFBJQTFBGQ TcLr212 + 3;;2 - 3+3+ 3+3+ Glenn0;;2+n2+n22 + n Steele-ND0;;2 + 3n2 AC Cora;2;2 - 3+3+ 3+3+ Faller0;;3+3+ 3+3+ RBO7;12 - ;3+3+ 3+3+ McKenzie;1;12 + n;2 Amidon;1;3+3+ 3+3+ Thatcher3+3+ 3+3+ 3+3+ 3+3+

7. P. triticina SSR Groups 2010 NA3 45% isolates 10 phenotypes NA5 53% isolates 23 phenotypes MLDSD- NA3 MFPSB- NA3 TDBJG - NA5 TDBGG- NA5 TCRKG- NA5

8. Top 10 Kansas Wheat Cvs 2011 TAM 111- Lr17 Fuller- Lr17,Lr39/41 Art- Lr17+ Postrock- Lr39/41 Santa Fe- Lr3,Lr17 TAM 112- Lr39/41 Jagger - Lr17 Armour - Lr39/41 Overley - Lr39/41 Blends

9. Whither Rust Pathology?? FungicideEffectiveness – WLR-WStR HeadlineExcellent QuadrisExcellent BumperVery good CarambaExcellent FolicurExcellent ProlineVery good PropimaxVery good ProsaroExcellent TiltVery good QuiltExcellent StrategoVery good TwinlineExcellent Moderate – High Rust pressure $4-8 Wheat/bushel $10-20 Fungicide/acre Susceptible Wheat Average >10% yield increase >93% chance of economic return with fungicide application