Presentation on theme: "The Case of Lactase Persistence Evolution in Humans slide version 1.0"— Presentation transcript:
1The Case of Lactase Persistence Evolution in Humans slide version 1.0
2About this Case:These slides were created by the Evo-Ed Project:Funding for the Evo-Ed Project is provided by the National Science Foundation and by Lyman Briggs College, Michigan State University.These slides are provided as a teaching resource. You are encouraged to modify them to meet your specific teaching and learning needs.Please adhere to the copyright conditions specified on the following slide.There is a reference slide at the end of the presentation that lists the sources for the images we have used in this presentation.If you would be willing to be in involved in our research study examining how the use of these case studies impacts learning, please contact us at
3Copyright: Creative Commons Attribution-NonCommercial-ShareAlike 3 Copyright: Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)You are free:to Share — to copy, distribute and transmit the workto Remix — to adapt the workUnder the following conditions:Attribution — You must attribute the work to the Evo-Ed Project at Michigan State University using the following url:Noncommercial — You may not use this work for commercial purposes.Share Alike — If you alter, transform, or build upon this work, you may distribute the resulting work only under the same or similar license to this one.With the understanding that:Waiver — Any of the above conditions can be waived if you get permission from Jim Smith, Merle Heidemann or Peter White at Michigan State University,Public Domain — Where the work or any of its elements is in the public domain under applicable law, that status is in no way affected by the license.Other Rights — In no way are any of the following rights affected by the license:Your fair dealing or fair use rights, or other applicable copyright exceptions and limitations;The author's moral rights;Rights other persons may have either in the work itself or in how the work is used, such as publicity or privacy rights.Notice — For any reuse or distribution, you must make clear to others the license terms of this work. The best way to do this is with a link to the web page
4IntroductionThese slides are provided as a teaching resource for the Lactase Persistence case as described on A fuller description of the case can be found on the website. Teaching notes can be found in the notes section beneath each slide when viewing the slides in “Normal View” in PowerPoint. To select this option in PowerPoint, go to the main menu, choose “View” and then “Normal.”
6Biology of the Digestive Tract Enterocytes (the cells that line the inside of digestive tract) are responsible for breaking down and absorbing nutrients from the food in the stomach and small intestine.
7First Food: Mother’s Milk The enterocytes of all infant mammals exhibit high levels of lactase during infancy, when milk is the main source of nutrition.Photo credit: Jim French, Flickr
8Lactase Unlocks an Energy Source Lactose is a disaccharide sugar found in milk.Lactase breaks down lactose intotwo monosaccharides, glucose and galactose.These simple sugars can be absorbed by cells in the small intestine and used as a source of energy.Lactase(enzyme)LactoseGlucoseGalactose
9Location of LactaseThis is the “brush border” of an enterocyte, which is the side of the enterocyte that comes in contact with the contents of the small intestine. Lactase is stained brown.
10Lactase Breaks Down Lactose GlucoseGalactoseLactoseLactaseINTESTINE CONTENTS ENTEROCYTE BLOODSTREAMLactase, PURPLE, is a transmembrane protein on the interior border of the enterocyte, YELLOW. When lactose comes into contact with its active site (depicted as an oddly shaped extension into the intestine contents, GREEN), it is broken down into Glucose and Galactose
11Glucose and Galactose are Absorbed INTESTINE CONTENTS ENTEROCYTE BLOODSTREAMSGLT1GLUT2SGLT1 (Sodium-Glucose Linked Transporter 1), ORANGE, transports Glucose or Galactose via facilitated diffusion from the intestine, GREEN, into the enterocyte, YELLOW. Then GLUT2 (Glucose Transporter 2), BLUE, transports glucose from the enterocyte to the bloodstream, RED.
12Lactase RegulationAlmost all known mammals – including 65% of humans – experience a decrease in lactase biosynthesis in the years after weaning. The regulation of lactase biosynthesis after weaning is the main factor that separates Lactase Persistent from Lactase Non-Persistent individuals.Definition: Weaning: the process of gradually replacing a infant mammal’s diet of breast milk with what will be its adult diet
13Why does this happen? Why can’t adult mammals digest milk? Lactase RegulationWhy does this happen? Why can’t adult mammals digest milk?
14Lactase RegulationThe decrease in lactase production after weaning is likely a matter of energy conservation at a cellular level:It takes energy to produce any enzyme, including lactase, the enzyme needed to digest milk.Typically, mammals do not consume milk once they have stopped nursing.Without milk consumption, energy spent producing lactase would be energy wasted at the cellular level.Therefore, over time, the more energetically favorable option has been selected for: a decrease in lactase production after weaning.This is an important point about evolution at the molecular scale. Be sure to stress cellular energetics as the “building blocks” of making the most efficient “machine,” or animal.
15Lactase not Produced in Adults What happens to humans then, if they continue to drink milk into adulthood?
16Lactase not Produced in Adults If undigested lactose passes into the large intestine, it will trigger the symptoms of Lactose Intolerance.1. The increased sugar concentration in the large intestine creates an osmotic gradient that draws water into the gut. This causes cramping and diarrhea.2. Bacteria in the large intestine digest the lactose as food, creating gaseous by-products like methane, carbon dioxide, and hydrogen. This leads to gas build-up and flatulence.
17Lactase PersistenceHOWEVER… 35% of humans do produce lactase after weaning, and are therefore able to continue to consume milk and other dairy products into adulthood.
18Discussion 1. How do you think this is possible? 2. What changed in order for these adult humans to be able to digest milk?
19Discussion 1. How do you think this is possible? 2. What changed in order for these adult humans to be able to digest milk?This is possible through Lactase Persistence, or the continued production of lactase at high levels throughout adulthood.The down-regulation of lactase biosynthesis that normally occurs must have been prevented or counteracted.
20Review What are enterocytes? Where is the lactase enzyme located? What does lactase do?Why can’t most adult mammals drink milk?What happens if they do?Why is lactase regulated?See slide 6See slides 5 and 9See slide 8, 10, 11See slide 13See slide 16See slide 14
23Lactase BiosynthesisTranscription (controlled by Transcription Factors)TranslationPost-Translational ModificationsExpression on the Cell Membrane
241. TranscriptionWithin the nucleus, the Lactase gene is transcribed into Lactase-mRNA by RNA polymerase. Note that other factors, also shown in blue, are important for the function of RNA polymerase.The rate of transcription is controlled by Transcription Factors shown here in red and green.Image Source:
25Transcription Factors A transcription factor (TF) is a protein that binds to a specific segment of DNA and influences the transcription of a gene.
26Transcription Factors Once bound to DNA, a Transcription Factor can attract the molecular machinery necessary for transcription. TFs can even attract other transcription factors (and they often do), forming a large transcription complex. We will focus on two different types of TFs, promotors and activators.
27PromotersPromoters, shown as a complex of proteins, bind to Promoter Sites on the DNA, and are responsible for initiating transcription by binding to RNA Polymerase and associated factors, as well as to other Promoters.The promoter is shown in green
28ActivatorsActivators bind to Enhancer Sites which are, particular stretches of DNA, and influence the probability and frequency of transcription by attracting more Promoters and other Transcription Factors to the transcription complex.
29ActivatorsEnhancer Sites may be far away from the start of a gene, but this allows Activators to cause large loops in the DNA, which can bring many distant but important transcription factors all together around one large transcription complex. This increases the rate of transcription of the gene.
30Why is this important?Because there is a mutation associated with Lactase Persistence that affects the binding ability of Transcription Factors.
31What Kind of Mutation is This? A Single Nucleotide Polymorphism, or SNP, is a type of mutation that involves changing one letter in the genetic code.One letter in billions of letters may seem insignificant, but a SNP can affect how strongly (and therefore how often) a Promoter or Activator will bind to that piece of DNA.This can have very profound effects on the transcription, translation, and expression of genes.
32The SNP in Lactase Persistence In lactase persistence an enhancer site located base pairs upstream from the Lactase gene has a “C” replaced by a “T.”This SNP increases how strongly and how often the transcription factor Oct1 binds to this site.As an activator, Oct1 causes more promoters to bind to the Lactase gene... Leading to more transcription of Lactase-mRNA!Information from: Lewinsky, Rikke H. et al. T DNA variant associated with lactase persistence interacts with Oct-1 and stimulates lactase promoter activity in vitro. Human Molecular Genetics, 2005, Vol. 14, No. 24, 3945–3953
33Normal Adult Mammals1After weaning, there is a decrease in promoter activity at the Lactase Gene2This leads to decreased transcription of the Lactase gene3The result is lower levels of Lactase in the enterocytes, and the inability to digest lactose in milkIt may be useful to present these slides side-by-side.Information from: Lewinsky, Rikke H. et al. T DNA variant associated with lactase persistence interacts with Oct-1 and stimulates lactase promoter activity in vitro. Human Molecular Genetics, 2005, Vol. 14, No. 24, 3945–3953
34Mutant (Lactase Persistent) *A mutation at an Enhancer site located base pairs upstream of the Lactase gene increases binding of an Activator called Oct11Oct1 attracts more Promoters to the Lactase gene throughout the individual’s adult life.2This prevents the decreased transcription of the Lactase gene that would normally happen.3The result is steady levels of Lactase in the enterocytes, and a retained ability to digest lactose in milk throughout adulthood.It may be useful to present these slides side-by-side.Information from: Lewinsky, Rikke H. et al. T DNA variant associated with lactase persistence interacts with Oct-1 and stimulates lactase promoter activity in vitro. Human Molecular Genetics, 2005, Vol. 14, No. 24, 3945–3953
35Notes for Previous Slide The ER and Golgi Apparatus process the polypeptide Pre-Pro-Lactase into its mature form, enzymatic Lactase (a protein).Within the Rough E.R. (Endoplasmic Reticulum):The signal sequence, whose purpose it was to attach the ribosome to the ER during translation, is cleaved off after translation.The membrane-bound polypeptide, now called Pro-lactase, is then dimerized (attached to another copy of itself.)Then a transport vesicle containing Pro-lactase blebs off the ER and travels to fuse with the Golgi Body.Within the Golgi Body:The “pro” subunit of Pro-Lactase prevents degradation and ensures proper folding of the polypeptide.This subunit is then cleaved off, leaving two Lactase polypeptides, which together make a mature Lactase enzyme.A vesicle containing the membrane-bound Lactase blebs off the Golgi…and travels to fuse with the cell membrane, which is the brush border membrane of enterocytes.
362. TranslationLactase-mRNA is translated by a membrane-bound ribosome into anamino acid called Pre-Pro-lactase.During translation, the polypeptide is fed into the ER(endoplasmic reticulum), but remains anchored in the lipidbilayer of the ER membrane after the ribosome hasdeparted.Image Source:
373. Post-Translational Modification 1. Rough E.R.2. Vesicle3. Golgi Body4. Vesicle5. Cell MembraneThe ER and Golgi Apparatus process the polypeptide Pre-Pro-Lactase into its mature form, enzymatic Lactase (a protein).Within the Rough E.R. (Endoplasmic Reticulum):The signal sequence, whose purpose it was to attach the ribosome to the ER during translation, is cleaved off after translation.The membrane-bound polypeptide, now called Pro-lactase, is then dimerized (attached to another copy of itself.)Then a transport vesicle containing Pro-lactase blebs off the ER and travels to fuse with the Golgi Body.Within the Golgi Body:The “pro” subunit of Pro-Lactase prevents degradation and ensures proper folding of the polypeptide.This subunit is then cleaved off, leaving two Lactase polypeptides, which together make a mature Lactase enzyme.A vesicle containing the membrane-bound Lactase blebs off the Golgi…and travels to fuse with the cell membrane, which is the brush border membrane of enterocytesNote: image does not perfectly depict cleavage, glycosylation, or dimerization. It does, however, outline the protein’s journey from Rough ER to Cell MembraneThis information is not integral to understanding the main point of the Molecular Genetics section, but is included for more advanced understanding of enzyme biosynthesis.
38Review What is Transcription? What are the different kinds of Transcription Factors, and what do they do?What is a SNP?How does the SNP associated with Lactase Persistence lead to increased transcription of Lactase in adulthood?See slide 22See slides 27-30See slide 34See slide 35, or 23-33
39Anthropology and Biogeography of Lactase Persistence
40The Neolithic Revolution Image source:The Neolithic revolution describes a period of time, between 12,000 and 6,000 years ago, during which humans around the world began transitioning from a hunter-gatherer lifestyle to a farming-herding lifestyle.
41Neolithic RevolutionThis time period saw a burst of innovation as humans developed new ways to interact with their environment, such as tools for planting and reaping crops, mills for grinding grains, and pottery for storage of food.Image source:
42Neolithic RevolutionThere were also conceptual innovations driven by this transition, such as the calendar, and the concepts of property and monetary systems. The ancient Egyptian calendar was divided into three seasons based on agricultural activities.Image source:
43PastoralismPastoralism, the cultural practice of milking livestock (such as goats, sheep, cows, and camels), was another innovation of the Neolithic Revolution. It was adopted in various cultures between 12,000 and 7,000 years ago.
44Milking Livestock and Drinking that Milk The Biocultural Coevolution theory proposes that pastoralism and lactase persistence coevolved. This means that they arose around the same time*, and both changes were reinforced by each other.*In evolutionary terms, “around the same time” can mean a couple thousands of years. Remember that the timescale for evolution is extraordinarily slow.Image source:
45How Did Lactase Persistence and Pastoralism Spread?
46Current Distribution of Lactase Persistence Fraction of adults with LP traitImage Source:
47Two Different Histories Lactase persistence and pastoralism arose and spread through Europe and Africa independently, an example of convergent evolution. Convergent Evolution is the independent evolution of similar features in separate lineages. This leads to two different “stories” of how Pastoralism and Lactase Persistence arose.
48The Story of LP in Europe The earliest evidence of pastoralism was discovered in the Middle East: bones of young cattle, slaughtered before their first birthdays, indicate that humans in the area had begun domesticating and milking cattle. The cattle bones found in the Middle East were about 10,500 years old.Image Source:
49Migration10,500years agoThe earliest domesticated cattle in Greece and the Balkan States (8,000 years old) were more closely related to the domesticated cattle from the Middle East than the wild cattle found in Europe at the time. This indicates that migrants from the Middle East brought their cattle with them.8,000Image source:
50Migration Because Middle Eastern cattle herders had more advanced 10,500years ago8,0007,5006,500Because Middle Eastern cattle herders had more advancedfood technology, they easily out-competed the localhunter-gatherers they encountered in Central and Northern Europe.
51An Apparent Paradox? What was going on? Anthropological evidence places the advent of pastoralism at 10,500 to 6,500 years ago.However, genetic research says the lactase persistence trait did not become widespread in Europe until 7,000 to 5,000 years ago.This suggests that for several thousand years some humans were milking sheep, goats, cows, or camels despite being unable to digest milk.What was going on?
52CheeseIt is likely that Neolithic humans fermented milk into cheese, which greatly reduced the lactose content of the dairy product, making it more accessible.Ancient pottery remnants like this, found in Northern Europe, were likely sieves used to strain and ferment milk into cheese
53A Brief History of Cheese Many Paleolithic and Neolithic cultures practiced the storage and transport of food and water in animal skins and intestines. When milk was introduced to the Neolithic diet in the Middle East it was likely stored in an inflated cow stomach, resulting in the separation of the curds and whey. Cheese is thought to have accompanied pastoralism since its cultural genesis, and is still very popular in European cultures.Image source:
54The Story of LP in Africa In Africa, lactase persistence evolved independently from the European lineage. The mutations responsible for lactase persistence are different.Prevalence of Lactase PersistencePrevalence of T SNPImage adapted from
55The Story of LP in Africa Neolithic humans in Africa experienced similar selective pressures to adopt pastoralism and evolve lactase persistence. Through slightly different mutations, G and G-13907, different peoples in Kenya and Sudan evolved lactase persistence via the same Oct1 transcription factor enhancer site.Image source:
56The Story of LP in Africa Notice how close the mutation (SNP) sites for Kenya and Sudan are to the European SNP, T All of these mutations affect the enhancer site of Oct1, the activator that increases promoter activity at the Lactase gene.Link back to Molecular Genetics section. Slide 34-35
57How Did Lactase Persistence Spread? Lactase persistence is thought to have arisen and spread through two types of natural selection: positive selection (selection for advantageous traits) and negative selection (selection against disadvantageous traits).Positive Selection and Negative Selection are not the same thing.Additional material may be required to better explain this if students are having trouble.
58How Did Lactase Persistence Spread? Negative SelectionPositive Selection(disadvantages of Lactose Intolerance)(advantages of Lactase Persistence)Individually: Non-LP individuals missed out on a potentially important source of nutrition and hydrationIndividually: Milk supplies protein, fat, sugar, and vitamins, and is dependable despite cold weather and/or bad cropsExperiencing painful, dehydrating symptoms upon consuming milk (see slide 15), which could be deadly.Neolithic women who could digest milk were estimated to produce 32% more offspring.Culturally: Milk is a more efficient protein source: it does not require killing livestock, yet the milk from one cow nearly equals the caloric value of the meat from a whole cow.Culturally: Without pastoralism, herders must slaughter their livestock to gain dietary protein from their meat.Information from The Milk Revolution, Andrew Curry
59How Did Lactase Persistence Spread? Lactase Persistence is a dominant trait. This is also an important aspect of how LP spread throughout the population over time.“Dominant trait” means that if one parent is lactase persistent, all the children will be lactase persistent.Image source:
60Review When did pastoralism originate in the Middle East? How did pastoralism spread in Europe?Why is cheese an important part of the history of Lactase Persistence?How did Lactase Persistence spread in Africa?What selective pressures drove the evolution of LP?See slide 48See slides 49-50See slides 51-53See slides 54-56See slide 58, 59
61Advanced Study Questions What is the Vitamin D/Cold Weather hypothesis concerning the latitude-associated distribution of Lactase Persistence?There is a culture in Africa with the ability to drink milk without ill effect despite not being lactase persistent. Who are they and how is this possible?These questions are provided for follow-up (extra) research on the Anthropology and Biogeography of lactase persistence.