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Amino Acid Enzymes Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase
Amino Acid Structure
Amino Acid Enzymes Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid
Peptone Iron Agar Deeps Peptone – protein rich in cysteine Iron - Fe
Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid H2S Fe (Iron) FeS (Ferrous Sulfide) Black
Amino Acid Enzymes Lysine decarboxylase Lysine Cadaverine + Carbon dioxide
Lysine Decarboxlyase Broth Lysine Fermentable sugar ph indicator Basic = purple Acid = Yellow Durham Tube Mineral Oil on top after inoculation (maintain an Anaerobic environment)
Lysine decarboxylase broth
Amino Acid Enzymes Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia
Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia FeCl2 (Ferric Chloride) HCl Green
Phenylalanine Agar Slants
Identifying Bacteria based on Enzymes and multiple test media
Protein lysis chapter 27 Page 207 Bacteria: – Escherichia coli – Staphylococcus aureus – Salmonella Medium: – Skim milk medium – Lysine decarboxylase broth.
Single Media & Multiple Tests
Lab #8. Review of Lab #7 - pH Indicators pH Indicator Very acidic AcidicNeutralBasic Phenol red- pH <6.8 = yellow pH = red pH >8.0 = magenta/
SINGLE MEDIA / MULTIPLE TESTS Medical Microbiology Laboratory MEDI 3101 Mr.Shadi Alashi.
TSI and Carbohydrate Tests
BIOCHEMICAL TESTING. Distinguishing Enterobacteriaceae Many found in the intestines of human or other mammals Varrying pathogenicity: Commensals, opportunists.
Physiological characteristics: Oxidative and fermentation tests
Bacteria identification : Media. What you have to know about the media What are the sources of C,H,N,O,P,S? What type of media is it? What are the indicators?
Exercise 40: Hydrolytic and Degradative Reactions
Lab 12 Goals and Objectives: Exercise 40: Hydrolytic and Degradative Reactions Read results: some will require additional reagents Exercise 41: Multiple.
TRIPLE SUGAR IRON AGAR TRIPLE SUGAR IRON AGAR PREPARED. BY: GHASSAN ABU HAJJAJ. SUPERVISOR: DR. Abdelraouf A. Elmanama.
Biochemical Activities of Microorganisms Part (2).
Media & Biochemical Tests
Lab 13 Goals and Objectives: Exercise 41: Multiple Test Media: Read and record results Exercise 71: Gram Negative Intestinal Pathogens Read and record.
Exercise 41: Multiple Test Media: Read and record results
Summary of Biochemical Tests in Microbiology
Glucose – a monosaccharide Lactose – a disaccahride glucose + galactose Fermentation can produce acid and/or gas A heterofermenter produces large amounts.
General Microbiology Laboratory Biochemical Tests.
Biochemical Tests Used for the Identification of Gram Negative Rods by Cyndie J. Hobson, MLS(ASCP) cm SM cm Carolinas College of Health Sciences School.
Introduction to Lab Ex. 16 Diagnostic Media Urea broth Sulfide Indole Motility Citrate.
University of Tabuk Faculty of Applied Medical Science Department of Medical Laboratory Technology Mr.AYMAN.S.YOUSIF M.SC IN Microbiology &IMMUNOLOGY Academic.
H 2 S production hydrogen sulfide production. Amino acid cystine (substrate) Cystine desulfhydrase – enzyme Hydrogen sulfide (product) Medium has iron.
Biochemical Characterization of Bacteria LECTURE 8: Microbiology and Virology; 3 Credit hours Atta-ur-Rahman School of Applied Biosciences (ASAB) National.
Lab #9. Review - pH Indicators pH Indicator Very acidic AcidicNeutralBasic Phenol red- pH <6.8 = yellow pH = red pH >8.0 = magenta/ hot pink.
Biochemical Tests Enterobacteriaceae
Enzymes that attack “N” substrates 1. Urease 2. Gelatinase 3. Nitrate Reductase.
Carbohydrates 1. Monosaccharides glucose, fructose, galactose 2. Disaccharides sucrose, maltose, lactose 3. Polysaccharides starch, glycogen, Agar.
Week 8 W Exp 22: Carbohydrate Fermentation Lab Two pg 158 Exp 23: Triple Sugar Iron Agar Test Lab Two pg 162 Charts New: Exp 24: IMViC Part A: Indole Test.
Media Preparation and Sterilization
PHT 381 Lab# 6 Biochemical reactions.
3 starch plates 5 urea broths (replaces urea slant)
Lab 10 BACTERIOLOGY OF THE GASTROINTESTINAL TRACT Lab Manual (p.67-82)
Media!. Figure 1.16 Bacterial colonies on agar Bacterium 1 Bacterium 2 Bacterium 3 Bacterium 4 Bacterium 5 Bacterium 6 Bacterium 7 Bacterium 8 Bacterium.
Lab. No. 7. II. Enterobacteriaceae It divided into two main groups: It divided into two main groups: According to their effect on lactose Lactose.
Lab. No. 4. Aerobic, non-fermentative, motile, oxidase-positive gram- negative bacilli. Aerobic, non-fermentative, motile, oxidase-positive gram- negative.
Microbiology Quiz Basic Lab Tests
Lab. No. 5. Gram-negative, non-spore-forming bacilli. Gram-negative, non-spore-forming bacilli. Their natural habitat is the intestinal tract of humans.
Lab #7. Microbial growth and metabolism So far what we know: Colony morphology and cell morphology (rod vs cocci) Motility Oxygen requirement Gram stain,
Exercise 39: Oxidation and Fermentation Tests
Lab. No. 4 (A). StaphylococciStreptococciMicrococci NeisseriaCorynbacterium Clostridum Bacillus Enterobacteriaceae Pseudomonas. Bacteria Gram’s Stain.
COMPONENTS PREPARATION INOCULATION Culture Media.
Biochemistry Class Nine. Macromolecules Microorganisms and their Identification Determination of pathogens responsible for infectious diseases Selection.
- It is nutritive ingredient needed by a particular m.o to enhance it growth under laboratory condition. -Different type of media: 1- Growth media(general.
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