2. Amino Acid Enzymes Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase

3. Amino Acid Structure

4. Amino Acid Enzymes Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid

5. Peptone Iron Agar Deeps Peptone – protein rich in cysteine Iron - Fe

6. Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid H2S Fe (Iron) FeS (Ferrous Sulfide) Black

7. Cysteine desulfhydrase

8. Amino Acid Enzymes Lysine decarboxylase Lysine Cadaverine + Carbon dioxide

9. Lysine Decarboxlyase Broth Lysine Fermentable sugar ph indicator Basic = purple Acid = Yellow Durham Tube Mineral Oil on top after inoculation (maintain an Anaerobic environment)

10. Lysine decarboxylase broth

11. Amino Acid Enzymes Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia

12. Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia FeCl2 (Ferric Chloride) HCl Green

13. Phenylalanine Agar Slants