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Amino Acid Enzymes Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase
Amino Acid Structure
Amino Acid Enzymes Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid
Peptone Iron Agar Deeps Peptone – protein rich in cysteine Iron - Fe
Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid H2S Fe (Iron) FeS (Ferrous Sulfide) Black
Amino Acid Enzymes Lysine decarboxylase Lysine Cadaverine + Carbon dioxide
Lysine Decarboxlyase Broth Lysine Fermentable sugar ph indicator Basic = purple Acid = Yellow Durham Tube Mineral Oil on top after inoculation (maintain an Anaerobic environment)
Lysine decarboxylase broth
Amino Acid Enzymes Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia
Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia FeCl2 (Ferric Chloride) HCl Green
Phenylalanine Agar Slants
Identifying Bacteria based on Enzymes and multiple test media Lab # 9 Medgar Evers College Prof. Victor Santos.
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Glucose – a monosaccharide Lactose – a disaccahride glucose + galactose Fermentation can produce acid and/or gas A heterofermenter produces large amounts.
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Lab #9. Review - pH Indicators pH Indicator Very acidic AcidicNeutralBasic Phenol red- pH <6.8 = yellow pH = red pH >8.0 = magenta/ hot pink.
Biochemical Tests. Fermentation Glucose acts as an electron donor to one or more of its organic products Glucose acts as an electron donor to one or more.
Biochemical Tests Enterobacteriaceae Dr.T.V.Rao MD 1.
Enzymes that attack “N” substrates 1. Urease 2. Gelatinase 3. Nitrate Reductase.
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Week 8 W Exp 22: Carbohydrate Fermentation Lab Two pg 158 Exp 23: Triple Sugar Iron Agar Test Lab Two pg 162 Charts New: Exp 24: IMViC Part A: Indole Test.
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Lab 11 Goals and Objectives: Exercise 39: Oxidation and Fermentation Tests Read results: some tubes will require additional reagents Do controls first.
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