Presentation on theme: "Algae from the Great Salt Plains, OK, USA: Unexpected Range of Diversity and Halotolerance Andrea Kirkwood & William Henley Dept. of Botany, Oklahoma State."— Presentation transcript:
Algae from the Great Salt Plains, OK, USA: Unexpected Range of Diversity and Halotolerance Andrea Kirkwood & William Henley Dept. of Botany, Oklahoma State Univ. Mark & Julie Buchheim Dept. of Biology, Univ. of Tulsa Other PIs: Robert Miller (OSU) & Mark Schneegurt (Wichita State Univ.)
~750 km Marine via migratory waterfowl Other Great Plains saline habitats Local soils and freshwater creeks Endemic for millenia (Permian brine) Sources of SP halotolerant microbes?
Clone Library vs. Isolates 16S rDNA clone library sequences closely matching culture isolates (Komvophoron sp.) Numerous clone library sequences with few cultured relatives
Future Work Compare clone libraries from soil/water to our culture isolates quant-/qualitatively, and use libraries to drive isolations. Compare morphological isolates vs. 16S/18S rDNA sequences and physiology: cryptic variation? Does salinity tolerance of algal/cyanobacterial isolates match field distributions? Characterize associations between algae/cyanos and bacteria Assess spatial/temporal changes in cyano/algal community (morphological ID? DGGE? ): is it predictable? Is there a quantifiable measure of microbial stress on the flats? What really matters – salt, UV, temp., nutrients?