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LB145 Sec 001-004. Today’s Outline Tutorial Reminder Homework #1: Results Homework #2: Assigned Organelles – Nucleus, RER, Golgi Apparatus, Cytoskeleton,

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Presentation on theme: "LB145 Sec 001-004. Today’s Outline Tutorial Reminder Homework #1: Results Homework #2: Assigned Organelles – Nucleus, RER, Golgi Apparatus, Cytoskeleton,"— Presentation transcript:

1 LB145 Sec

2 Today’s Outline Tutorial Reminder Homework #1: Results Homework #2: Assigned Organelles – Nucleus, RER, Golgi Apparatus, Cytoskeleton, SER, Ribosomes, Lysosomes, Peroxisomes, Vacuoles, Mitochondria, Chloroplasts Match’Em Exercise

3 Last chance to register clickers! Register your clicker by the end of this week. Exam Review -Tuesday Review Session (Trivia Game). -Special office hours: Wednesday morning.

4 Homework 1: Average = 6.24/7.00 (Excellent!) Grades are on ANGEL along with my comments. Next time – if you do not submit it to TurnItIn, it will not receive a score. Next time – academic integrity… – You were shown in class a good example of what a violation of academic integrity looks like. Come see me if you have more questions or if you would like to see the de-identified example again.

5 This is an example of Academic Dishonesty 1. Academic dishonesty includes (but is not limited to): 1.3. plagiarism: representing the words or ideas of another as one's own in any academic exercise (e.g., papers, computer programming assignments, class presentation, or laboratory reports); 1.4. submitting the same paper, or substantially similar papers, to meet the requirements of more than one course without the consent of all instructors concerned; 2. Instructors in Lyman Briggs are expected to take appropriate action in all instances of academic dishonesty…. In cases of academic dishonesty, an instructor may take action consistent with his/her syllabus, including giving a failing grade on the assignment, giving a lower or failing grade for the course, and/or recommending additional disciplinary action. [See website addendum under Academic Honesty]

6 Homework #2: Northern European Folklore: Wehe dem Kind, das beim Kuß auf die Stirn salzig schmekt, er ist verhext und muss bald sterbe! "Woe to the child which when kissed on the forehead tastes salty. He is bewitched and soon will die!”

7 -Nucleus -Ribosomes -Rough Endoplasmic Reticulum (RER) -Smooth Endoplasmic Reticulum (SER) -Golgi Apparatus -Peroxisomes -Lysosomes -Vacuoles -Mitochondria -Chloroplasts -Cytoskeleton Eukaryotes: Organelles to Live By

8 The Nucleus

9 Ribosomes

10 Ribosome and rRNA rRNA is made in the nucleus. Small and Large Subunit proteins are transported INTO the nucleus. Where the ribosome is assembled. (below: protein in purple, rRNA in orange and yellow) Eukaryotes have FOUR strands of rRNA. 5S 5.8S 28S 18S Prokaryotes have THREE strands of rRNA. 5S 23S 16S What does this have to do with the lab?

11 Keeping RNAs Straight mRNA – MESSANGER RNA – Transcribed from DNA in nucleus. – Transported out into cytosol. – Interacts with ribosome where translation takes place and a protein is formed. rRNA – RIBOSOMAL RNA – Transcribed from DNA in nucleus – Binds to large and small ribosomal subunits in nucleus – acts as structural support. – Exits nucleus (bound to ribosomal subunits) via nuclear pore. tRNA – TRANSFER RNA – Transcribed from DNA in nucleus – Exits into cytosol via nuclear pore – Picks up a specific amino acid (covalent bond) in cytosol and transfers it to growing polypeptide chain.

12 Some proteins are made in the cytoplasm (cytosol) on “free” ribosomes 9/abiopb/flow.jpg

13 The Rough ER

14 Some proteins are made on “bound” ribosomes Campbell 8e, Fig They enter the endomembrane system

15 The Golgi Apparatus

16 The Endomembrane System: Manufacturing and Shipping Proteins

17 Insulin is Secreted via the Endomembrane System Campbell 8e, Fig. 6.16

18 Cytoskeleton n Microtubules (green) n Microfilaments (red) n Nucleus (blue)

19 Molecular Components of the Cytoskeleton

20 A Motor Protein Generates Motile Forces (Class of 1984)

21 The Smooth ER

22 Smooth ER Synthesize fatty acids and phospholipids. Breaks down toxins/poisonous lipids. Reservoir Ca+ ions.

23 Peroxisomes

24 Center of oxidation reactions. – E.g. glyoxisomes oxidizing fat to store it as usable energy. Break down very long fatty acid chains to shorter carbon chains. – These shorter chains are eventually shipped to mitochondrion and used to produce ATP.

25 Lysosomes

26 Digestion packets full of enzymes. Found in animal cells. Fuse with vesicles or old organelles. Often a very acidic environment. – Low pH. – Lots and lots of hydrogen ions.

27

28 Vacuole

29 Vacuoles In plants and fungi. Sometimes used to aid in digestion. Store water and help cell maintain volume.

30 Mitochondria

31 Mitochondria – Generates ATP from Glucose Campbell 8e, Fig. 6.17

32 Chloroplasts

33 Chloroplasts – Generate G3P (glucose precursor) from sunlight and CO 2 Campbell 8e, Fig. 6.18

34 Activity – Cells and Organelles

35 Coming Up Next Week: Homework 2 is due on Tuesday – The Case of Cystic Fibrosis – Upload to TurnItIn (no identifying marks) – Bring a copy to class (last four #s of PID) Tuesday: – Evaluate HW – Review for Exam 1. Exam #1 is on the 24 th.


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