1. MICROBIOLOGY LAB SAFETY BY JEREMY MARION JMARION@NU.EDU

2. RULE #1 -Know the safety rules and procedures that apply to the work being done. -Review Potential Hazards and take appropriate safety precautions before beginning any new operations. -Practical Jokes or other behavior that might confuse, startle, or distract another lab student is not allowed.

3. RULE #2 Shower Head MSDS Data First Aid Kit Eye Wash Station Shower Lever Fire Blanket Spill Kit -Know where emergency equipment is located and how to properly use it. -Familiarize yourself with emergency routes. -In case of evacuation, SHUT OFF BUNSEN BURNERS BEFORE LEAVING.

4. RULE #3 -Become Familiar with the types of protective equipment available and understand their use and limitations -Safety glasses are required whenever hazardous chemicals or equipment is being used, prepared, or demonstrated -Lab coats and Gloves, including disposables, are highly recommended.

5. RULE #4 -No food or beverages are permitted in the Lab -Do not apply cosmetics in areas where chemicals are being used or stored

6. RULE #5 Confine long hair, jewelry, and loose clothing when working in the laboratory. Appropriate shoes are required in the lab at all times. Skin should be covered at all times.

7. RULE #6 -Follow accepted waste disposal procedures -Work defensively with Flammables

8. LAB STATION SUPPLIES 2 Lab Coats 2 Wire Loops 2 Wire Needles 1 Slide Container 1 Box of Slides 2 Clothes Pins 1 Ruler 1 Wax Pencil

9. CHEMICAL SUPPLIES 2 - 250 mL beakers 1 Bottle 95% Ethanol 1 Dropper Bottle Methylene Blue 1 Dropper Bottle Safranin 1 Dropper Bottle Crystal Violet 1 Dropper Bottle Iodine

10. PROPER STORAGE OF MICROSCOPE 1.Eyepiece facing handle 2.Objective Lens set to 10x 3.Stage lowered and set back as far as possible 4.Cord loosely folded. Do not wrap the Cord around the base or neck of the Microscope. 5.Place Microscope in Cabinet with handle facing outwards

11. WASTE DISPOSAL Glassware that has not touched Hazardous Waste Culture Tubes and Plates Hazardous Waste Slides Glassware that has touched Hazardous Waste

12. WASTE DISPOSAL CONT… Broken Glass Only Biohazard Specimen Autoclaved Materials