1. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 1 Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA). Microbe-I JEM MICROBE-I 31407 Instructor:Takashi Yamasaki Satoshi Kawaguchi (yamasaki.takashi@jaxa.jp) (kgucci@jamss.co.jp)

2. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 2 Objectives After this lesson the trainee will be able to: - Explain the science objective of the Microbe-I experiment. - List onboard outcomes from performing Microbe-I experiment. - List two sampling methods of Microbe-I experiment. - Explain what crew has to do to Microbial Detection Sheet after sampling to prevent bio contamination. - Explain what crew has to do with BZK Wipe after Microbial Detection Sheet sampling to prevent bio contamination. - Explain what crew must NOT contaminate throughout sampling. - Identify the area on Microbial Detection Sheet Yeast and Mold where crew must NOT touch. - Identify the area on Blue/White Wipes where crew must NOT touch. - Perform Microbe-I operations according to ODFs without instructor’s assistance.  Microbe-I Overview  Microbe-I Operations Overview  Key points during Microbe-I operations  Microbe-I Operations

3. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 3 Lesson Outline Contents: - Microbe-I Overview - Microbe-I Operations Flow - Key Points during Microbe-I Operations - Appendix Page: 4 5 6 7

4. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 4  Microbial Detection Sheet Yeast and Mold  White Wipe in White Tube  Blue Wipe in Blue Tube  Photos of Microbial Detection Sheet Yeast and Mold and sampling locations The purpose of this experiment is to monitor microbes in Kibo which may affect the health of crew members. Microbe-I Overview Microbe-I Science Objective Onboard Outcomes from Microbe-I Experiment Microbial Detection Sheet Yeast and Mold White Wipe and White Tube (for freezing) Blue Wipe and Blue Tube (for refrigeration)

5. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 5 Microbial Detection Sheet Sampling Microbial Detection Sheet Preparation Microbial Detection Sheet Sampling Photographing Microbial Detection Sheet Microbial Detection Sheet Incubation (7 days) Blue/White Wipe Sampling Blue/White Wipe MELFI Insertion Microbe-I Operations Flow :Crew operations LEGEND: Microbe-I Operations Flow Microbial Detection Sheet MELFI Insertion Photographing Sampling Location

6. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 6 Key Points during Microbe-I Operations CategoryEquipmentKey Points Crew Safety Bio Contamination due to leakage of Microbial Detection Sheet culture media Microbial Detection Sheet - After Microbial Detection Sheet is used, cover film must be applied. - Microbial Detection Sheet must be contained in Ziplock bag. (2-level containment: cover film and ziplock bag) BZK Wipes- Use BZK Wipe to clean residual Microbial Detection Sheet culture media. - Discard BZK Wipe into two Ziplock bags. (2-level containment) Time Critical OperationN/A Dos and Don’tsGloves- Do NOT contaminate gloves by bare skin or mucosa membrane throughout sampling. Microbial Detection Sheet - Do NOT touch non-woven fabric or inner side of cover film where non- woven fabric will contact to prevent contamination. Blue/White Wipe - Do NOT touch Blue/White Wipe except cap to prevent contamination. Difficulty in OpsN/A Possible Mis-OpsN/A CAUTION: Crew safety may be changed in future. (Final Payload Safety Review Panel has not been held yet.)

7. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 7 Appendix - Diffuser - Acronyms

8. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 8 Diffuser

9. Training Purposes Only JEM MICROBE-I 31407 JMU-081192 (Ver. 1.2) 9 Acronyms BZK Wipes MELFI Benzalkonium Chloride Antiseptic Towelette Minus Eighty degree Laboratory Freezer for ISS - Benzalkonium chloride antiseptic towelettes are alcohol-free wet wipes used for general antiseptic cleansing.